Characterizing bean pod rot in Arkansas and Missouri

Green beans are an important crop grown for processing in both Arkansas and Missouri. Green beans are harvested mechanically using non-selective picking fingers. Harvested beans are then transported in bulk to processing plants that are located at various locations throughout the midSouth. Thus, the crop is managed for high quality, avoiding pod blemishes caused by insects and diseases. One of the consistent quality problems that affect Arkansas and Missouri green bean crops is pod rot. Two of the causal agents of pod rot that have been reported by researchers and vegetable companies alike are Pythium aphanidermatum and an unidentified Phytophthora sp. In this study, 15 growers’ fields were selected and soil samples (at planting), pod samples (at harvest), and environmental data were taken from each field. Disease incidence for field sites ranged from 0 to 7.3%. Pathogens associated with pod rot were Sclerotinia sclerotiorum, Rhizoctonia solani, a Phytophthora sp., and Pythium spp. The two suspected causal agents for pod rot, Pythium and Phytophthora spp., were found in all but one of the 12 field sites assessed for pod rot. Pythium inoculum potential, as determined by a baiting technique, was not a good indicator of pod rot incidence. In addition, soil temperature and water were not associated with pod rot. Pods collected at harvest having symptoms of pod rot were either in direct contact with the soil, senescing leaf tissue, or other diseased pods

Polarizability of molecular hydrogen

Static dipole electric polarizabilities of molecular hydroge

Orbital Variability in the Eclipsing Pulsar Binary PSR B1957+20

We have conducted timing observations of the eclipsing millisecond binary pulsar PSR~B1957+20, extending the span of data on this pulsar to more than five years. During this time the orbital period of the system has varied by roughly $\Delta P_b/P_b = 1.6 \times 10^{-7}$, changing quadratically with time and displaying an orbital period second derivative $\ddot P_b = (1.43 \pm 0.08) \times 10^{-18}\,$s$^{-1}$. The previous measurement of a large negative orbital period derivative reflected only the short-term behavior of the system during the early observations; the orbital period derivative is now positive and increasing rapidly. If, as we suspect, the PSR~B1957+20 system is undergoing quasi-cyclic orbital period variations similar to those found in other close binaries such as Algol and RS CVn, then the $0.025\,M{_\odot}$ companion to PSR~B1957+20 is most likely non-degenerate, convective, and magnetically active.Comment: 9 pages, 3 figures, LaTeX, submitted ApJL 13 Dec. 1993, arz-00

Selection of neutralizing antibody escape mutants with type A influenza virus HA-specific polyclonal antisera: possible significance for antigenic drift

Ten antisera were produced in rabbits by two or three intravenous injections of inactivated whole influenza type A virions. All contained haemagglutination-inhibition (HI) antibody directed predominantly to an epitope in antigenic site B and, in addition, various amounts of antibodies to an epitope in site A and in site D. The ability of untreated antisera to select neutralization escape mutants was investigated by incubating virus possessing the homologous haemagglutinin with antiserum adjusted to contain anti-B epitope HI titres of 100, 1000 and 10000 HIU/ml. Virus-antiserum mixtures were inoculated into embryonated hen's eggs, and progeny virus examined without further selection. Forty percent of the antisera at a titre of 1000 HIU/ml selected neutralizing antibody escape mutants as defined by their lack of reactivity to Mab HC10 (site B), and unchanged reactivity to other Mabs to site A and site D epitopes. All escape mutant-selecting antisera had a ratio of anti-site B (HC10)-epitope antibody[ratio]other antibodies of [gt-or-equal, slanted]2·0[ratio]1. The antiserum with the highest ratio (7·4[ratio]1) selected escape mutants in all eggs tested in four different experiments. No antiserum used at a titre of 10000 HIU/ml allowed multiplication of any virus. All antisera used at a titre of 100 HIU/ml permitted virus growth, but this was wild-type (wt) virus. We conclude that a predominant epitope-specific antibody response, a titre of [gt-or-equal, slanted]1000 HIU/ml, and a low absolute titre of other antibodies ([less-than-or-eq, slant]500 HIU/ml) are three requirements for the selection of escape mutants. None of the antisera in this study could have selected escape mutants without an appropriate dilution factor, so the occurrence of an escape mutant-selecting antiserum in nature is likely to be a rare event

Improving "bag-of-keypoints" image categorisation: Generative Models and PDF-Kernels

In this paper we propose two distinct enhancements to the basic ''bag-of-keypoints" image categorisation scheme proposed in [4]. In this approach images are represented as a variable sized set of local image features (keypoints). Thus, we require machine learning tools which can operate on sets of vectors. In [4] this is achieved by representing the set as a histogram over bins found by k-means. We show how this approach can be improved and generalised using Gaussian Mixture Models (GMMs). Alternatively, the set of keypoints can be represented directly as a probability density function, over which a kernel can be de ned. This approach is shown to give state of the art categorisation performance