Artifactual immunofluorescent labelling in yeast, demonstrated by affinity purification of antibody

In the course of making antibodies against various yeast ( S. cerevisiae ) proteins, we have noted that it is common to observe reactivity of rabbit sera with a number of extraneous bands on Western transfers of yeast proteins. The pattern of reactive bands can change within a period of weeks, even when the rabbit has not been injected with antigen. A simple method of affinity purification, using antigen bound to nitrocellulose, is employed to remove the reactivity with these extraneous bands from immune sera. The importance of affinity purification is demonstrated by our attempts to immunolocalize a 55 kd yeast protein (p55). Immune serum stains yeast cells to give a striking pattern of spots and blotches not seen with preimmune serum. However, affinity purification of anti-p55 antibody shows that this pattern is not due to staining by anti-p55 antibody; rather the pattern is due to staining left in the serum depleted of anti-p55 antibody.Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/38511/1/320030202_ftp.pd

Low-scale warped extra dimension and its predilection for multiple top quarks

Within warped extra dimension models that explain flavor through geometry, flavor changing neutral current constraints generally force the Kaluza-Klein scale to be above many TeV. This creates tension with a natural electroweak scale. On the other hand, a much lower scale compatible with precision electroweak and flavor changing neutral current constraints is allowed if we decouple the Kaluza-Klein states of Standard Model gauge bosons from light fermions $c_{\rm light}\simeq c_b\simeq 0.5$ bulk mass parameters). The main signature for this approach is four top quark production via the Kaluza-Klein excitations' strong coupling to top quarks. We study single lepton, like-sign dilepton, and trilepton observables of four-top events at the Large Hadron Collider. The like-sign dilepton signature typically has the largest discovery potential for a strongly coupled right-handed top case (M_{KK} \sim 2-2.5 \TeV), while single lepton is the better when the left-handed top couples most strongly (M_{KK} \sim 2 \TeV). We also describe challenging lepton-jet collimation issues in the like-sign dilepton and trilepton channels. An alternative single lepton observable is considered which takes advantage of the many bottom quarks in the final state. Although searches of other particles may compete, we find that four top production via Kaluza-Klein gluons is most promising in a large region of this parameter space.Comment: 35 pages, 8 figures. discussions improved, references adde

Top Quark Physics at the LHC: A Review of the First Two Years

This review summarizes the highlights in the area of top quark physics obtained with the two general purpose detectors ATLAS and CMS during the first two years of operation of the Large Hadron Collider LHC. It covers the 2010 and 2011 data taking periods, where the LHC provided pp collisions at a center-of-mass energy of sqrt(s)=7 TeV. Measurements are presented of the total and differential top quark pair production cross section in many different channels, the top quark mass and various other properties of the top quark and its interactions, for instance the charge asymmetry. Measurements of single top quark production and various searches for new physics involving top quarks are also discussed. The already very precise experimental data are in good agreement with the standard model.Comment: 107 pages, invited review for Int. J. Mod. Phys. A, v2 is identical to v1 except for the addition of the table of content

Monte Carlo Exploration of Warped Higgsless Models

We have performed a detailed Monte Carlo exploration of the parameter space for a warped Higgsless model of electroweak symmetry breaking in 5 dimensions. This model is based on the $SU(2)_L\times SU(2)_R\times U(1)_{B-L}$ gauge group in an AdS$_5$ bulk with arbitrary gauge kinetic terms on both the Planck and TeV branes. Constraints arising from precision electroweak measurements and collider data are found to be relatively easy to satisfy. We show, however, that the additional requirement of perturbative unitarity up to the cut-off, $\simeq 10$ TeV, in $W_L^+W_L^-$ elastic scattering in the absence of dangerous tachyons eliminates all models. If successful models of this class exist, they must be highly fine-tuned.Comment: 26 pages, 7 figures; new fig and additional text adde

Heavy-light decay topologies as a new strategy to discover a heavy gluon

We study the collider phenomenology of the lightest Kaluza-Klein excitation of the gluon, G*, in theories with a warped extra dimension. We do so by means of a two-site effective lagrangian which includes only the lowest-lying spin-1 and spin-1/2 resonances. We point out the importance of the decays of G* to one SM plus one heavy fermion, that were overlooked in the previous literature. It turns out that, when kinematically allowed, such heavy-light decays are powerful channels for discovering the G*. In particular, we present a parton-level Montecarlo analysis of the final state Wtb that follows from the decay of G* to one SM top or bottom quark plus its heavy partner. We find that at \sqrt{s} = 7 TeV and with 10 fb^{-1} of integrated luminosity, the LHC can discover a KK gluon with mass in the range M_{G*} = (1.8 - 2.2) TeV if its coupling to a pair of light quarks is g_{G*qqbar} = (0.2-0.5) g_3. The same process is also competitive for the discovery of the top and bottom partners as well. We find, for example, that the LHC at \sqrt{s} = 7 TeV can discover a 1 TeV KK bottom quark with an integrated luminosity of (5.3 - 0.61) fb^{-1} for g_{G*qqbar} = (0.2-0.5) g_3.Comment: 36 pages, 13 figures. v2: a few typos corrected, comments added, version published in JHE

Theories for the Fermi Scale

I give a short review of our present understanding of new theories of the electroweak scale, with emphasis on recent progress. Plenary talk at the EPS 2007 Conference at Manchester.Comment: 14 pages, EPS 2007 Conferenc

Potent CD8+ T-cell immunogenicity in humans of a novel heterosubtypic influenza A vaccine, MVA-NP+M1.

BACKGROUND: Influenza A viruses cause occasional pandemics and frequent epidemics. Licensed influenza vaccines that induce high antibody titers to the highly polymorphic viral surface antigen hemagglutinin must be re-formulated and readministered annually. A vaccine providing protective immunity to the highly conserved internal antigens could provide longer-lasting protection against multiple influenza subtypes. METHODS: We prepared a Modified Vaccinia virus Ankara (MVA) vector encoding nucleoprotein and matrix protein 1 (MVA-NP+M1) and conducted a phase I clinical trial in healthy adults. RESULTS: The vaccine was generally safe and well tolerated, with significantly fewer local side effects after intramuscular rather than intradermal administration. Systemic side effects increased at the higher dose in both frequency and severity, with 5 out of 8 volunteers experiencing severe nausea/vomiting, malaise, or rigors. Ex vivo T-cell responses to NP and M1 measured by IFN-γ ELISPOT assay were significantly increased after vaccination (prevaccination median of 123 spot-forming units/million peripheral blood mononuclear cells, postvaccination peak response median 339, 443, and 1443 in low-dose intradermal, low-dose intramuscular, and high-dose intramuscular groups, respectively), and the majority of the antigen-specific T cells were CD8(+). CONCLUSIONS: We conclude that the vaccine was both safe and remarkably immunogenic, leading to frequencies of responding T cells that appear to be much higher than those induced by any other influenza vaccination approach. Further studies will be required to find the optimum dose and to assess whether the increased T-cell response to conserved influenza proteins results in protection from influenza disease

Exploring the Higgs Portal with 10/fb at the LHC

We consider the impact of new exotic colored and/or charged matter interacting through the Higgs portal on Standard Model Higgs boson searches at the LHC. Such Higgs portal couplings can induce shifts in the effective Higgs-gluon-gluon and Higgs-photon-photon couplings, thus modifying the Higgs production and decay patterns. We consider two possible interpretations of the current LHC Higgs searches based on ~ 5/fb of data at each detector: 1) a Higgs boson in the mass range (124-126) GeV and 2) a `hidden' heavy Higgs boson which is underproduced due to the suppression of its gluon fusion production cross section. We first perform a model independent analysis of the allowed sizes of such shifts in light of the current LHC data. As a class of possible candidates for new physics which gives rise to such shifts, we investigate the effects of new scalar multiplets charged under the Standard Model gauge symmetries. We determine the scalar parameter space that is allowed by current LHC Higgs searches, and compare with complementary LHC searches that are sensitive to the direct production of colored scalar states.Comment: 27 pages, 11 figures; v2: references added, correction to scalar form factor, numerical results updated with Moriond 2012 data, conclusions unchange