A system to enrich for primitive streak-derivatives, definitive endoderm and mesoderm, from pluripotent cells in culture

Two lineages of endoderm develop during mammalian embryogenesis, the primitive endoderm in the pre-implantation blastocyst and the definitive endoderm at gastrulation. This complexity of endoderm cell populations is mirrored during pluripotent cell differentiation in vitro and has hindered the identification and purification of the definitive endoderm for use as a substrate for further differentiation. The aggregation and differentiation of early primitive ectoderm-like (EPL) cells, resulting in the formation of EPL-cell derived embryoid bodies (EPLEBs), is a model of gastrulation that progresses through the sequential formation of primitive streak-like intermediates to nascent mesoderm and more differentiated mesoderm populations. EPL cell-derived EBs have been further analysed for the formation of definitive endoderm by detailed morphological studies, gene expression and a protein uptake assay. In comparison to embryoid bodies derived from ES cells, which form primitive and definitive endoderm, the endoderm compartment of embryoid bodies formed from EPL cells was comprised almost exclusively of definitive endoderm. Definitive endoderm was defined as a population of squamous cells that expressed Sox17, CXCR4 and Trh, which formed without the prior formation of primitive endoderm and was unable to endocytose horseradish peroxidase from the medium. Definitive endoderm formed in EPLEBs provides a substrate for further differentiation into specific endoderm lineages; these lineages can be used as research tools for understanding the mechanisms controlling lineage establishment and the nature of the transient intermediates formed. The similarity between mouse EPL cells and human ES cells suggests EPLEBs can be used as a model system for the development of technologies to enrich for the formation of human ES cell-derived definitive endoderm in the future.Sveltana Vassilieva, Hweee Ngee Goh, Kevin X. Lau, James N. Hughes, Mary Familari, Peter D. Rathjen and Joy Rathje

Retrieving biomedical images through content-based learning from examples using fine granularity

Session: Data Mining IITraditional content-based image retrieval methods based on learning from examples analyze and attempt to understand high-level semantics of an image as a whole. They typically apply certain case-based reasoning technique to interpret and retrieve images through measuring the semantic similarity or relatedness between example images and search candidate images. The drawback of such a traditional content-based image retrieval paradigm is that the summation of imagery contents in an image tends to lead to tremendous variation from image to image. Hence, semantically related images may only exhibit a small pocket of common elements, if at all. Such variability in image visual composition poses great challenges to content-based image retrieval methods that operate at the granularity of entire images. In this study, we explore a new content-based image retrieval algorithm that mines visual patterns of finer granularities inside a whole image to identify visual instances which can more reliably and generically represent a given search concept. We performed preliminary experiments to validate our new idea for content-based image retrieval and obtained very encouraging results.published_or_final_versio

Evolution and CNO yields of Z=10^-5 stars and possible effects on CEMP production

Our main goals are to get a deeper insight into the evolution and final fates of intermediate-mass, extremely metal-poor (EMP) stars. We also aim to investigate their C, N, and O yields. Using the Monash University Stellar Evolution code we computed and analysed the evolution of stars of metallicity Z = 10^-5 and masses between 4 and 9 M_sun, from their main sequence until the late thermally pulsing (super) asymptotic giant branch, TP-(S)AGB phase. Our model stars experience a strong C, N, and O envelope enrichment either due to the second dredge-up, the dredge-out phenomenon, or the third dredge-up early during the TP-(S)AGB phase. Their late evolution is therefore similar to that of higher metallicity objects. When using a standard prescription for the mass loss rates during the TP-(S)AGB phase, the computed stars lose most of their envelopes before their cores reach the Chandrasekhar mass, so our standard models do not predict the occurrence of SNI1/2 for Z = 10^-5 stars. However, we find that the reduction of only one order of magnitude in the mass-loss rates, which are particularly uncertain at this metallicity, would prevent the complete ejection of the envelope, allowing the stars to either explode as an SNI1/2 or become an electron-capture SN. Our calculations stop due to an instability near the base of the convective envelope that hampers further convergence and leaves remnant envelope masses between 0.25 M_sun for our 4 M_sun model and 1.5 M_sun for our 9 M_sun model. We present two sets of C, N, and O yields derived from our full calculations and computed under two different assumptions, namely, that the instability causes a practically instant loss of the remnant envelope or that the stars recover and proceed with further thermal pulses. Our results have implications for the early chemical evolution of the Universe.Comment: 12 pages, 13 figures, accepted for publication in A&

InGaAsP p-i-n photodiodes for optical communication at the 1.3-µm wavelength

The preparation and properties of Cd-diffused p-n homojunction InGaAsP photodiodes designed specifically for operation at the 1.3-µm wavelength are described. At a reverse bias of 10 V, the dark current of these diodes was as low as 15 pA. The peak responsivity at 1.3-µm wavelength was 0.7 A/W. An impulse response (full width at half maximum) of 60 ps and a 3-dB bandwidth of 5.5 GHz were achieved.

Analytic structure of radiation boundary kernels for blackhole perturbations

Exact outer boundary conditions for gravitational perturbations of the Schwarzschild metric feature integral convolution between a time-domain boundary kernel and each radiative mode of the perturbation. For both axial (Regge-Wheeler) and polar (Zerilli) perturbations, we study the Laplace transform of such kernels as an analytic function of (dimensionless) Laplace frequency. We present numerical evidence indicating that each such frequency-domain boundary kernel admits a "sum-of-poles" representation. Our work has been inspired by Alpert, Greengard, and Hagstrom's analysis of nonreflecting boundary conditions for the ordinary scalar wave equation.Comment: revtex4, 14 pages, 12 figures, 3 table

Medium: An exploratory study of PR professionals in Taiwan

This paper seeks to explore the attitudes and practices of public relations (PR) professionals in using the Internet as a PR medium in Taiwan. Specifically, the paper addresses three research questions: (1) What do PR professionals think about the function of the Internet and the roles of online communication in comparison with those of offline communication in Taiwan? (2) Will IT-related corporations be more likely to have a wider scope of Internet applications than non-IT related corporations? (3) Do IT corporations perceive the role of PR differently from non-IT related corporations? Using a semi-structured questionnaire, we conducted in-depth interviews with eight corporations’ PR and marketing department professionals in Taiwan from May 18- June 18, 2001. The findings show that (1) Although the Internet is widely used by PR professionals, it does not alter media strategies significantly; (2) PR professionals in IT corporations tend to practise more Internet communication functions than those in non-IT related corporations; (3) IT corporations are more likely to support the management role of PR professionals, as compared to those in non-IT related businesses. The study suggests that the applications of the Internet vary according to industry type, business strategies and roles of the PR professionals within an organisation