Human Pappilomavirus Genotype in Cervical Tissue of Patients with Cervical Intraepithelial Neoplasia (CIN) 1, CIN 2, and CIN 3

Objectives: to determine the genotype of HPV in patients with precancerous lesions of cervical tissue.Materials and Methods: An observational study with cross sectional study of patients paraffin block CIN1, CIN2, CIN3 was conducted in Dr Soetomo Hospital. HPV DNA was extracted from paraffin blocks, then performed PCR and genotyping of HPV. The sample consisted of 28 patients with cervical tissue paraffin blocks CIN1, CIN2 and CIN3. Patients aged between 26-74 years (standard deviation 10,12).Results: HPV genotypes that infect patients with CIN1 were HPV16 and 18, CIN2 were HPV16 and 52 and CIN3 were HPV16, 67, and combined infection HPV16/67 and HPV52/67. HPV genotypes in a single infection were 26/28 (HPV16, HPV18, HPV52 and HPV67), and multiple infections were 2/28 (HPV16/67 and HPV52/67).Conclusion: The most dominant HPV genotypes infect patients with precancerous lesions of the cervix were HPV16, HPV67, HPV52, and HPV18

Genetic Transformation of Rhodesgrass (Chloris Gayana Kunth.) by Particle Bombardment

Rhodesgrass (Chloris gayana Kunth) has been cultivated as one of the most important warm-season grasses in the world. One of the major limitations for cattle production on forage grasses, especially warm-season grasses is poor digestibility if compared to temperate grasses (Gondo et al., 2003). It is believed that the low digestibility of warm-season grasses is due to high lignin contents (Akashi et al., 2003). Recently, modification of the lignin content of plants appears to be feasible using genetic engineering strategies. We have established a methodology for high-frequency somatic embryogenesis and multiple shoot formation from seed-derived shoot apical meristems in rhodesgrass. Also, we have studied several factors involved in particle bombardment transformation

TEKNIK PENANGANAN KOMPONEN SISA PADA OPERASI INDUSTRI PENANGKAPAN IKAN YANG BERBASIS DI PELABUHAN PERIKANAN PANTAI BLANAKAN

Penelitian bertujuan menganalisis keragaan komponen sisa dan menyusun teknik penanganannya pada operasi industri penangkapan ikan yang berbasis di PPP Blanakan.  Metode yang digunakan adalah metode deskripsi, model PPA, dan analisis porsi tertimbang. Komponen sisa dari kelebihan stok perbekalan pada operasi industri penangkapan ikan adalah sisa BBM (rata-rata 38,5 l/kapal), sisa air tawar (rata-rata 117,5 l/kapal), dan sisa es balok (rata-rata 5,3 balok/kapal). Adapun komponen sisa dari dampak aktivitas operasi kapal adalah tumpahan BBM dan oli, serta sampah melaut, terutama kemasan plastik. Teknik produksi bersih untuk penanganan komponen sisa pada operasi industri penangkapan ikan yang berbasis di PPP Blanakan adalah (a) memberi pelatihan perencanaan cermat perbekalan BBM (rethink), (b) mengurangi perbekalan es balok (reduce), (c) penggunaan kembali sisa air tawar untuk membersihkan peralatan (reuse), (d) memperbaiki instalasi yang bocor (refine) dan memberi pelatihan peningkatan kesadaran akan dampak tumpahan BBM dan oli (rethink), dan (e) mengurangi perbekalan berkemasan plastik (reduce). Kata kunci: air tawar, industri penangkapan ikan, komponen sisa, model PPA, pelabuhan perikanan, produksi bersi

Penguatan Cahaya Pada Bagan Menggunakan Reflektor Kerucut Sebagai Upaya Meningkatkan Hasil Tangkapan Cumi-cumi (Light Strengthening on Lift Net with Conical Reflectors to Squid Catch Improvement)

Study of light strengthening on lift net with conical reflectors was conducted in Kao Bay waters. Three units of lift net were operated 14 nights at the full moon condition. Every lift nets were completed with three different kind of lamp cover, called tudung, reflector αr23,3o and αr32,6o. Fishing operation time of lift net were divided into two intervals of time i.e 20.00-01.00 and 01.00-05.00 WIT (East Indonesian Timezone). Total yield of lift net with reflector αr23,3o was 5.774 kg (41,45%), while that of the lift net with reflector αr 32,6o was 4.977 kg (35,72%), and that of total yield of the lift net with tudung was 3.180 kg (22,83 %). Fishing operation time of lift net at 01.00-05.00 WIT (East Indonesian Timezone) produced 12.661 kg (91 %) weight of total catch, higher than fishing operation of lift net at 20.00-01.00 am that produced 1.270 kg (9 %) weight of total catch. However, statistical analysis concluded that design of the reflector did not significantly affect the catch per trip (Pvalue > 0,05) while fishing time significantly affected the catch per trip (Pvalue < 0,05)

Skrining Kanker Serviks Dengan Pemeriksaan Pap Smear Di Puskesmas Tanah Kali Kedinding Surabaya Dan Rumah Sakit Mawadah Mojokerto

Tujuan: Penelitian ini bertujuan untuk mengetahui prevalensi lesi prekanker serviks dengan cara melakukan skrining kanker serviks menggunakan pemeriksaan Pap smear.Bahan dan Metode: Penelitian ini merupakan penelitian observasional deskriptif dengan metode pendekatan cross sectional yang dilakukan pada 140 wanita, terdiri dari 90 orang dari Puskes-mas Tanah Kali Kedinding Surabaya, dan 50 orang dari Rumah Sakit Mawadah Mojokerto, dengan usia 20-70 tahun. Pemeriksaan spesimen sitologi serviks dengan pengecatan Papaniculaou dan klasifikasi hasil pemeriksaaan sitologi berdasar-kan klasifikasi Papaniculaou dan sistim Bethesda. Pemeriksaan inspeksi visual asam asetat (IVA) adalah metode lain untuk skrining kanker serviks dilakukan dengan mengoleskan asam asetat 5% pada area serviks dan melakukan pengamatan satu menit kemudian.Hasil: Hasil pemeriksaaan Pap smear menunjukkan Papaniculaou kelas I (sama dengan normal pada klasifikasi sistim Bethesda) yaitu 12.1%, kelas II (sama dengan NILM pada klasifikasi sistim Bethesda) yaitu 86, 4%, dan kelas III (sama dengan LSIL pada klasifikasi sistim Bethesda) yaitu 1,4%. Hasil pemeriksaan IVA menunjukkan 6,43% positif dan 93,57% negatif. Hasil pemeriksa-an IVA positif terdapat pada 9/140 orang yang merupakan proses keradangan dan infeksi, bukan merupakan lesi prekanker.Simpulan: Prevalensi lesi prekanker yaitu 1,4%. Pemeriksaan IVA menunjukkan hasil positif semu yang disebabkan oleh proses radang atau infeksi pada serviks

Novel ultrasonographic Halo Score for giant cell arteritis:assessment of diagnostic accuracy and association with ocular ischaemia

OBJECTIVES: Ultrasound of temporal and axillary arteries may reveal vessel wall inflammation in patients with giant cell arteritis (GCA). We developed a ultrasound scoring system to quantify the extent of vascular inflammation and investigated its diagnostic accuracy and association with clinical factors in GCA. METHODS: This is a prospective study including 89 patients suspected of having GCA, of whom 58 had a confirmed clinical diagnosis of GCA after 6 months follow-up. All patients underwent bilateral ultrasound examination of the three temporal artery (TA) segments and axillary arteries, prior to TA biopsy. The extent of vascular inflammation was quantified by (1) counting the number of TA segments and axillary arteries with a halo and (2) calculating a composite Halo Score that also incorporated the thickness of each halo. RESULTS: Halo counts and Halo Scores showed moderate diagnostic accuracy for a clinical diagnosis of GCA. They correlated positively with systemic inflammation. When compared with the halo count, the Halo Score correlated better with C-reactive protein (CRP) levels and allowed to firmly establish the diagnosis of GCA in more patients. Higher halo counts and Halo Scores were associated with a higher risk of ocular ischaemia. They allowed to identify subgroups of patients with low risk (≤5%) and high risk of ocular ischaemia (>30%). CONCLUSIONS: Ultrasound halo scoring allows to quantify the extent of vascular inflammation in GCA. Extensive vascular inflammation on ultrasound may provide strong diagnostic confirmation and associates with ocular ischaemia in GCA