Cytotoxic Interactive Effects of Dentin Bonding Components on Mouse Fibroblasts

Previous studies have shown a wide range of pulpal reactions to dentin bonding systems and a poor correlation between in vitro and in vivo toxicity of dentin bonding agents. Because dentin bonding agents are composed of multiple components which may diffuse through dentin, we hypothesized that these components may cause cytotoxicity through interactive (synergistic) effects. We investigated the cytotoxicities of four dentin bonding components-HEMA, Bis-GMA, TEGDMA, and UDMA-and interactive effects for three binary combinations of the dentin bonding components-HEMA and Bis-GMA, Bis-GMA and TEGDMA, and TEGDMA and UDMA. Cytotoxicities to Balb/c 3T3 mouse fibroblasts were measured by the MTT assay. Concentrations which caused 50% toxicity compared with controls (TC50 values) were compared, and the interactive effects were determined by evaluation of the differences between observed and expected MTT activities of the cells. The ranks of toxicity of the dentin bonding components in terms of TC50 values were as follows: Bis-GMA > UDMA > TEGDMA >>> HEMA (least toxic) after 24- and 72-hour exposures. As binary combinations, the three combinations of dentin bonding components interacted in three ways—synergism, additivism, and antagonism-which were influenced by the concentrations of both components. The longer period of exposure resulted in a significant increase in the cytotoxicity of the dentin bonding components and combinations. The findings indicate that both exposure time and the interactions between the dentin bonding components may be important parameters in determining the cytotoxicity of dentin bonding agents in vivo.Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/66489/2/10.1177_00220345950740091601.pd

Polymerized bonding agents and the differentiation in vitro of human pulp cells into odontoblast-like cells.

International audienceObjectives: Odontoblasts are highly differentiated post-mitotic cells, which under pathological conditions such as carious lesions and dental injuries may degenerate and be replaced by other pulp cells. We have recently shown that this physiological event can be reproduced in an in vitro assay system, but is highly modified by the presence of unpolymerized resinous monomers. Our hypothesis was that the photopolymerization of the monomers in the bonding agents might abolish these negative effects. The purpose of this study was to evaluate the effects of polymerized dentin bonding agents, through dentin slices, on odontoblast differentiation in vitro. Methods: Pulp cells were obtained from human third molars. They were used to study the effects of four dentin bonding agents through 0.7 mm dentin slices which served as a barrier between the bonding agents and the culture medium. The media containing the bonding agents' extracts were added at non-toxic concentrations onto the cultured cells. Immunohistochemistry was performed to study the differentiation of pulp fibroblasts into odontoblasts under these conditions by evaluating the expression of several odontoblast specific genes. Results: Pulp fibroblasts cultivated under these conditions synthesized type I collagen, osteonectin, dentin sialoprotein and nestin at the same level as in control cultures. Moreover, pulp cells synthesized a mineralized nodular extracellular matrix. Expression of these proteins was higher in the cells contributing to the nodule formation. In addition, except nestin, all these proteins were expressed in the mineral nodules. Significance: This work shows the lack of effects of photopolymerized bonding agents, through dentin slices, on cytodifferentiation of secondary odontoblasts

Risco climático do feijão da seca no Estado de Goiás, sob preparo de solo convencional e plantio direto Climatic risk of rainfed beans in the State of Goiás, Brazil, under conventional and no-till systems

Na região dos Cerrados, os agricultores têm adotado o sistema plantio direto (SPD) como forma de proteger o ambiente e dar sustentabilidade à exploração agrícola. Neste sistema, a cobertura do solo pela palhada pode minimizar o risco climático das culturas de sequeiro, pela redução do déficit hídrico. O objetivo deste trabalho foi comparar o risco climático do feijão da "seca" no Estado de Goiás, sob preparo de solo convencional e plantio direto. Foram utilizados o modelo BIPZON para o cálculo do balanço hídrico e um sistema de informações geográficas para a espacialização dos resultados, além de identificadas as áreas de baixo, médio e alto risco climático para esta cultura, para o SPD, com 50 e 100% de cobertura do solo pela palhada e para o sistema convencional de preparo de solo. Verificou-se que a utilização do SPD causou aumento das áreas de menor risco e prolongamento do período favorável de semeadura a esta cultura, em relação ao sistema de preparo convencional. O efeito foi mais pronunciado com 100% de cobertura do solo.<br>In the Cerrado Region, farmers have been adopting the no-till system, to protect the soil and give sustainability to agriculture. In that system, soil covered by crop residues can minimize climatic risks by reducing water deficit of crops sowed in late season. The objective of this study was to compare the climatic risk of dry season common beans grown in the State of Goiás, under conventional and no-till systems. The BIPZON model was used to calculate the water balance. Geographic Information System was used for the characterization of the results in time and space. Areas of low, medium and high climatic risks were identified for the no-till system, with 50 and 100% of soil coverage by crop residues, as well as for the conventional till system. It was verified that the use of no-till system increased the areas with lower risks and extended the period of sowing for the bean crop in relation to conventional till system. The effect was higher with 100% soil covered with crop residues