Cholera in children in Karachi from 1990 through 1995: A study of cases admitted to a tertiary care hospital

Although cholera is an endemic disease in Bangladesh, India and other countries, it was never a significant cause of gastroenteritis in Pakistan before 1988. Since then, cases of cholera are identified each year, both in adults and children in Pakistan. In order to see the contribution of Vibrio cholerae as a cause of gastroenteritis in children, we reviewed the cases of cholera admitted in the pediatric ward of the Aga Khan University Hospital, Karachi, Pakistan. Of 4346 children hospitalized with gastroenteritis during 1990 through 1995, 348 children (8%) were confirmed to have cholera. The youngest child with cholera was seven days old. The mean age was 31 +/- 34 months. The cases of cholera were received from all over the city. Most cases were due to Vibrio cholerae Ogawa biotype ELTOR but the new strain, i.e., Vibrio cholerae 0139 was isolated in 14% cases in 1994. The sensitivity of Vibrio cholerae has also changed. In 1994, the organisms were resistant to commonly recommended antibiotics, i.e., tetracycline, ampicillin and erythrocin but sensitive to ceftrioxone, cefixime, ofloxacin and nalidixic acid. Adequate measures to improve hygiene and sanitation and supply of safe potable water is needed to prevent any future epidemic of cholera in the city

Fast Food Trend Analysis by Evaluating Factors Leading to Customer Satisfaction

A fast food restaurant is a growing business and with the time evolving the pattern of eating habits is changing. Therefore, many people have started opting for fast food which is an appealing phenomenon for them. This research is conducted to evaluate the pattern of fast food and the customer satisfaction encompassing various factors. The analysis of the customer satisfaction encompasses independent variables which leads to consumer satisfaction and showcases the fast food trend. It is Karachi centric; target sample is from South Urban Region by the customers.The variables of this study are Quality of Service, Pricing, Ambiance and Quality of Food which are considered as independent. Age and Gender are part of Demographics. According to the IBIS World report on “Industry Market Research Report 2018”, the Global Food Industry has grown over the period of five years despite the change in factors such as consumer tastes and a recovering global economy. With the increase in disposable income the spending increased by the consumers on luxuries such as dinning out. In result of this the fast food operators started to gain benefits by providing full-service restaurants. The trend of Five years revealed growth by 3.5% in the Global Fast Food industry by generating revenue of $668bn in 2018 (IBISWORLD 2018). Since the trend of five years revealed the growth in fast food restaurant chains similarly this research has revealed the trend of fast food restaurant in Karachi Urban South region. McDonalds and KFC are the most preferred fast food chains because their customers are satisfied with the ambiance, pricing and quality of food which drives them to opt for the particular restaurant. For the results generation regression analysis have been applied which revealed significance of three independent variables and the interferential and descriptive analysis have also been implied. Keywords: customer satisfaction, price, quality of food, and ambiance DOI: 10.7176/JMCR/55-06 Publication date: April 30th 201

Aluminosilicates and yeast-based mycotoxin binders: Their ameliorated effects on growth, immunity and serum chemistry in broilers fed aflatoxin and ochratoxin

The aim of this study was to evaluate the effects of commercial toxin binders on growth performance, organ morphology, immunity and serum biochemistry in broilers. Dietary treatments consisted of the negative control (NC): experimental diet with aflatoxin B1 <20 parts per billion (ppb), ochratoxin A (OTA) <5 ppb; control (C) experimental diet without toxin binder; Z1: toxin binder 1 g/kg of zeta plus; Z2: toxin binder 2 g/kg of zeta plus; TX1: toxin binder 1 g/kg of Toxfin Dry; and TX2: toxin binder 2 g/kg of Toxfin Dry). Except for NC, all diets contained 57 ppb aflatoxin B1 and 23 ppb ochratoxin A. Feed intake was higher in the TX1, TX2, NC, Z2 and Z1 treatments than in the control. Weight gain was higher in Z2, TX2, Z1, TX1 and NC than in C. Feed conversion ratio (FCR) was poor in C. The control had the highest liver weight, though the weights of the spleen, kidneys and hearts of the birds were similar in all treatments. Gizzard weight, thymus weight, and bursa of Fabricius were lowest in C. The weight of the pancreas was similar among treatments. The antibody titres against new castle disease were higher in treatments Z2, Z1, TX2, TX1 and NC than in C. Urea and creatinine concentrations, and aspartate aminotransferase activity in serum were similar among treatments, whereas the serum alanine transaminase activity was higher in C than in Z1, TX1, TX2, Z2 and NC. It was concluded that growth rate, FCR and immunity indices were improved in broilers fed toxin binder. At lower levels of mycotoxin in feed, 1 g/kg of toxin binder (clay based or yeast based) was sufficient to ameliorate the adverse effects of aflatoxin B1 and OTA, whereas at higher levels of mycotoxins, supplementation of toxin binder should be increased.Keywords: alanine aminotransferase, aspartate aminotransferase, carcass characteristics, growth performance, toxin binders, urea, creatinin

Promoting female participation in professional development programmes

A recent publication of the Ministry of Education, Islamabad, shows that the province of Sindh has a population of over 30 million. The overall literacy rate in this province is 46.7 %, while the female literacy rate is 35.4%. However, in the rural areas of Sindh the female literacy rate is 13.11% against the male literacy rate of 52.1%. There are many social, cultural and economic factors that inhibit females from availing opportunities on an equal basis with the other segments of society. The literature also reveals that due to the above-mentioned factors females are confined to play a passive role in general and in the education sector in particular. According to a recent UNDP publication, there is considerable disparity between males and females in terms of professional development. Considering the importance of professional development of females in the education sector in Sindh, the Aga Khan University Institute for Educational Development (AKU-IED), under the Pakistan Non- Government Initiatives (PNI-II), took an initiative to launch a project titled ‘Strengthening the Capacity of NGOs/CBOs’. The project targeted to provide opportunities of professional development to 258 individuals, but the actual number of those who graduated was 388, considerably exceeding the planned target. Out of 388, 68 % (262) were females, which show the enthusiasm and commitment of the participants, collaborating NGOs/CBOs and AKU-IED towards the professional development of females. Through this initiative, females were trained as ‘agents of change’ to motivate their communities towards education of their children, in particular the girl child. This presentation unfolds the learning experiences of the USAID Project-III team in promoting female participation in professional development programmes at AKU-IED

DEVELOPMENT AND VALIDATION OF HPLC METHOD FOR QUANTITATIVE ESTIMATION OF NIMBOLIDE IN BULK AND PHARMACEUTICAL DOSAGE FORM

Objective: The present study was aimed to a simple, new, rapid and highly sensitive Reverse Phase - High Performance Liquid Chromatographic (RP-HPLC) method has been developed and an assay was validated for the quantitative estimation of nimbolide in solid dosage form.Methods: The chromatographic separation was achieved on an Agilent 1200 series HPLC system C18 (250 mm x 4.6 mm x 2.5 μ) column packing by using isocratic mobile phase consisting of acetonitrile: water (90:10 v/v), flow rate was adjusted to 1.0 ml/min at a fixed wave length of 207 nm.Results: The nimbolide was eluted at 2.880 ± 0.05 min and established a dynamic range of linearity over the concentration range of 3.125-200 ppm/ml (r2 = 0.9997 ± 0.005). The lower limit of detection and quantification was 0.007 ppm/ml and 0.021 ppm/ml respectively. The method was validated as per ICH guidelines. The accuracy of the method was determined by the recovery studies and the mean recovery was obtained 98.27%. Moreover the method was shown good reproducibility and recovery with percent relative standard deviation less than 2%.Conclusion: Rapid, efficient and sensitive RP-HPLC method was developed for the estimation of nimbolide from the perspective of reducing the cost of analysis and time and thus by saving laboratory resources.Â

Genome analysis of amaranths: determination of inter- and intra-species variations

Amaranths are an important group of plants and include grain, vegetable and ornamental types. Despite the economic importance of the amaranths, there is very little information available about the extent and nature of genetic diversity present in the genus Amaranthus at molecular level. We now report the randomly amplified polymorphic DNA (RAPD) profiles of different species of Amaranthus as well as different accessions of the species. These RAPD analyses have been carried out using 65 arbitrary sequence decamer primers. From the RAPD data, an UPGMA dendrogram illustrating the inter-as well as intra-species relationships has been computed. The putative hybrid origin of A.dubious from A. hybridus and A. spinosus is also ruled out by the RAPD data. The trends of species relationships amongst the amaranths determined by RAPDs is consistent with their cytogenetic and evolutionary relationships that have already been determined

The Antioxidant Activity of the Leaves of Barleria grandiflora Dalz. (Acanthaceae)

Aqueous and hydro alcoholic extracts of the leaves of Barleria grandiflora Dalz. were evaluated for the antioxidant activity by the FTC and TBA methods. The results obtained in the present study indicate that the leaves of Barleria grandiflora are potential source of natural antioxidants. Initial phytochemical screenings of the extracts have shown the presence of flavanoids, tannins, saponins, carbohydrates and aminoacid

Elucidating the Performance Limitations of Alkaline Electrolyte Membrane Electrolysis: Dominance of Anion Concentration in Membrane Electrode Assembly

Anion exchange membrane water electrolyzers (AEMWEs) offer a cost-effective technology for producing green hydrogen. Here, an AEMWE with atmospheric plasma spray non-precious metal electrodes was tested in 0.1 to 1.0 M KOH solution, correlating performance with KOH concentration systematically. The highest cell performance was achieved at 1.0 M KOH (ca. 0.4 A cm−2 at 1.80 V), which was close to a traditional alkaline electrolysis cell with ≈6.0 M KOH. The cell exhibited 0.13 V improvement in the performance in 0.30 M KOH compared with 0.10 M KOH at 0.5 A cm−2. However, this improvement becomes more limited when further increasing the KOH concentration. Electrochemical impedance and numerical simulation results show that the ohmic resistance from the membrane was the most notable limiting factor to operate in low KOH concentration and the most sensitive to the changes in KOH concentration at 0.5 A cm−2. It is suggested that the effect of activation loss is more dominant at lower current densities; however, the ohmic loss is the most limiting factor at higher current densities, which is a current range of interest for industrial applications