39 research outputs found
Enchytraeus albidus Microarray: Enrichment, Design, Annotation and Database (EnchyBASE)
Enchytraeus albidus (Oligochaeta) is an ecologically relevant species used as standard test organisms for risk assessment. Effects of stressors in this species are commonly determined at the population level using reproduction and survival as endpoints. The assessment of transcriptomic responses can be very useful e.g. to understand underlying mechanisms of toxicity with gene expression fingerprinting. In the present paper the following is being addressed: 1) development of suppressive subtractive hybridization (SSH) libraries enriched for differentially expressed genes after metal and pesticide exposures; 2) sequencing and characterization of all generated cDNA inserts; 3) development of a publicly available genomic database on E. albidus. A total of 2100 Expressed Sequence Tags (ESTs) were isolated, sequenced and assembled into 1124 clusters (947 singletons and 177 contigs). From these sequences, 41% matched known proteins in GenBank (BLASTX, e-value≤10-5) and 37% had at least one Gene Ontology (GO) term assigned. In total, 5.5% of the sequences were assigned to a metabolic pathway, based on KEGG. With this new sequencing information, an Agilent custom oligonucleotide microarray was designed, representing a potential tool for transcriptomic studies. EnchyBASE (http://bioinformatics.ua.pt/enchybase/) was developed as a web freely available database containing genomic information on E. albidus and will be further extended in the near future for other enchytraeid species. The database so far includes all ESTs generated for E. albidus from three cDNA libraries. This information can be downloaded and applied in functional genomics and transcription studies
Toxic interactions between energetic compounds and cyclodextrins
NRC publication: Ye
Ecotoxicological evaluation of 2,4,6-trinitrotoluene (TNT) and cyclodextrin mixtures using laboratory tests
NRC publication: Ye
Toxic interactions between energetic compounds and cyclodextrins
NRC publication: Ye
Ecotoxicological effects of recalcitrant environmental pollutants on two species of soil invertebrates (Enchytraeus albidus and Eisenia andrei)
NRC publication: Ye
Characterization of dinitrotoluenes and some of their reduced metabolites using aquatic-based toxicity tests
NRC publication: Ye
Ecotoxicity characterization of dinitrotuluenes and some of their reduced metabolites
NRC publication: Ye
Ecotoxicological evaluation of 2,4,6-TNT and cyclodextrins mixtures using laboratory test
NRC publication: Ye