A survey of ectoparasites on the gills, skin and fins of oreochromis Niloticus at Bagauda fish farm, Kano, Nigeria

Six hundred and two specimens of Oreochromis niloticus from Bagauda fish farm, Kano were examined for ectoparasites. Nine hundred and fifty six ectoparasites were found on two hundred and fourteen (35.6%) specimens. Piscine parasites recovered were protozoa and trematodes namely, Ichthyophthirius spp, Myxobolus spp, Clinostomum spp and Euclinostomum spp. Gill infestation was maintained to be higher throughout the year, than that on the skin and fins, and it was found to be statistically different by DMRT at 5% level of probability. Generally trematodes had higher frequency than protozoa in both the seasons (25.58%, 36.33% and 4.30%, 11.00% respectively). Frequency of protozoa infestation is higher in wet Season 11.00% than in dry season 4.30% so is the case with the trematodes, 36.33% in wet season and 25.5% in dry season

Distribution and abundance of freshwater snails in Warwade Dam, Dutse, Northern Nigeria

Preliminary investigation in August, 2017 reported the presence of Lymnaea natalensis, Bellamya unicolor, Melanoides tuberculata and Bulinus globosus in order of increasing abundance and distribution in Warwade dam, Dutse, Jigawa State, Nigeria. A follow up study was carried out from April to October, 2019 to reveal further details on the abundance and distribution of freshwater snails in relation to some physiochemical factors of the dam. Four sampling sites; human activity, vegetation cover, lentic and lotic were selected for the study along the bank of the dam. Freshwater snails were collected using long handled scoop net with mesh 0.2mm complemented by hand picking methods in the four sampling sites. Water samples from the sampling sites were analyzed in the laboratory using standard procedures. A total of 2,027 of freshwater snails belonging to ten species were identified. Bulinus globosus 12(0.6%) and Lymnaea natalensis 12(0.6%) had the lowest abundance and distribution while Melanoides tuberculata 1553(76.6%) had the highest. Snail abundance was highest in site characterized by human activities (670) followed by vegetation (482), lotic (442) and lentic (433) waters. Most of the physico-chemical factors measured appeared to favour the growth and survival of fresh water snails. pH (p = 0.01), water current (p = <0.01) and magnesium ion concentration (p = < 0.01) varied significantly across the four sites. Only calcium ion concentration was significantly associated with snail abundance (p = 0.04). Snail abundance showed weak positive relationship with water temperature, color, turbidity and concentration of magnesium ion. The dam habours about ten species of freshwater snails in different abundance and distribution with M. tuberculata being the most abundant throughout the period of investigation. The dominance of M. tuberculata over other species particularly those of medical and veterinary importance could have positive implication for their control in the dam

Prevalence of Schistosomiasis in Warwade Community, Jigawa State, Nigeria

There is currently no documented literature on the status of transmission of schistosomiasis in Warwade community situated near a dam. This study was designed to determine the prevalence of schistosomiasis in the community. A total of 300 urine and stool samples from randomly selected participants were analysed for S. haematobium and S. mansoni using sedimentation and kato-katz techniques respectively. A pre-tested structured questionnaire was administered to all the participants and in the case of minors their parents/guardians. The data was analysed using Statistical Package for Social Sciences version 25 to determine if there was any significant difference between schistosomiasis and demographic characteristics of participants as well as the association between schistosomiasis and risk factors at P<0.05. The results revealed an overall prevalence of 20.3% with urinary and intestinal schistosomiasis having 12.3% and 8% respectively .Out of every 8 persons in the community one had urinary schistosomiasis while in every 12 persons, one had intestinal and in every 5 persons, one had at least either of the species. Urinary schistosomiasis was higher in females, but was the opposite in intestinal infection. Schistosomiasis infection was highest among children ≤ 18 years and ≤40 years and the opposite was the case for intestinal schistosomiasis, where 19 to 29 years had the highest prevalence. Age and occupation were the risk factors associated with schistosomiasis infection at P<0.05. All the positive samples for urinary schistosomiasis subjected to Polymerase Chain Reaction (PCR) amplification were positive. This study showed that Warwade community is endemic for schistosomiasis. Keywords: Sedimentation, Kato-katz, schistosomiasis, endemi

RESPONSE OF MAIZE (Zea mays L.) GENOTYPES GROWTH CHARACTERS UNDER DROUGHT AND HEAT STRESS CONDITIONS EVALUATED IN SUDAN SAVANNA, NIGERIA

Drought stress and heat stress are two major limiting factors affecting maize productivity in the tropical regions. High temperatures and changes in rainfall pattern can cause significant decline in maize yields under rain fed conditions with Africa being one of the worst affected areas. Heat tolerance can be accomplished through genetic management approach. The aim of the research is to assess the extent of variation in tolerance to drought stress and heat tolerant stress and their performance on growth and yield characters. The trials were conducted on genetic analysis of Maize (Zea mays L.) inbred lines under combined (drought and heat stress) conditions. The parental materials comprises eight inbred lines that were crossed in a partial diallel pattern; thereafter, the checks, parents and resultant F1 generations were evaluated at two locations, Kano University of Science and Technology and farmers field at Dambatta local government area Kano state during 2021 dry seasons. The experiment was laid out in a randomized complete block design and replicated three times. The results indicated the crossing of P4 X P9 and P3 X P4 to produce significantly to produce significantly better growth attributes like plant height ear height, plant aspect and grain yield. These parental lines might be used in maize breeding programs in Nigeria as sources of drought and or heat tolerance

RESPONSE OF MAIZE (Zea mays L.) GENOTYPES GROWTH CHARACTERS UNDER DROUGHT AND HEAT STRESS CONDITIONS EVALUATED IN SUDAN SAVANNA, NIGERIA

Drought stress and heat stress are two major limiting factors affecting maize productivity in the tropical regions. High temperatures and changes in rainfall pattern can cause significant decline in maize yields under rain fed conditions with Africa being one of the worst affected areas. Heat tolerance can be accomplished through genetic management approach. The aim of the research is to assess the extent of variation in tolerance to drought stress and heat tolerant stress and their performance on growth and yield characters. The trials were conducted on genetic analysis of Maize (Zea mays L.) inbred lines under combined (drought and heat stress) conditions. The parental materials comprises eight inbred lines that were crossed in a partial diallel pattern; thereafter, the checks, parents and resultant F1 generations were evaluated at two locations, Kano University of Science and Technology and farmers field at Dambatta local government area Kano state during 2021 dry seasons. The experiment was laid out in a randomized complete block design and replicated three times. The results indicated the crossing of P4 X P9 and P3 X P4 to produce significantly to produce significantly better growth attributes like plant height ear height, plant aspect and grain yield. These parental lines might be used in maize breeding programs in Nigeria as sources of drought and or heat tolerance

CORRELATION AND PATH COEFFICIENT ANALYSIS BETWEEN GRAIN YIELD AND SOME GROWTH AND YIELD COMPONENTS OF MAIZE (ZEA MAYS L.) GENOTYPE AS INFLUENCED BY DROUGHT AND HEAT STRESS CONDITIONS IN SUDAN SAVANNAH OF NIGERIA

Experiments were conducted at the Teaching and Research farm of the Department of Crop Science Kano University of Science and Technology, Wudil and on a farmer’s field at Dambatta Local Government Area, with eight maize genotypes to assess the character associations among the genotypes for yield attributes. The experiment was conducted during February to June 2021 and also 2022, in a lattice design with three replications in both years and locations and are made to determine the performance of the genotypes in interacting environments. The genotypes differed significantly for most of the studied traits. Grain yield was significantly associated with plant height, anthesis silking interval, days to maturity, ear height, ears per plant, grain weight per plant in a positive direction and associated negatively with tassel blast, barren plant and leaf senescence. Path co-efficient analysis revealed that the maximum positive direct contribution towards yield was through grain weight per plant, plant height, and ears per plant whereas ear height showed negative direct contribution to grain yield due to negative indirect effects of several other parameters such as ear height via grain weight per plant, plant height via grain weight per plant

Different patterns of pfcrt and pfmdr1 polymorphism in Plasmodium falciparum isolates from Tehama region, Yemen

Introduction. Despite the efforts of the malaria control programme, malaria morbidity is still a common health problem in Yemen, with 60% of the population at risk. Plasmodium falciparum is responsible for 99% of malaria cases. The emergence in Yemen of parasite resistance to chloroquine (CQ) prompted the adoption of artemisinin combination therapy (ACT) in 2009, which involves the use of artesunate plus sulphadoxine-pyrimethamine (AS + SP). However, CQ was retained as the drug of choice for vivax malaria. To assess the impact of the change in the malaria treatment policy five years after its introduction, the present study investigated the mutations in the CQ resistance transporter (pfcrt) and multidrug resistance 1 (pfmdr1) genes. Method. A molecular investigation of 10 codons of pfcrt (72–76, 220, 271, 326, 356, and 371) and five codons of pfmdr1 (86, 184, 1034, 1042, and 1246) was conducted on P. falciparum isolates from districts with the highest malaria endemicity in the Hodeidah and Al-Mahwit governorates in Tehama region, Yemen. A total of 86 positive cases of falciparum monoinfection were investigated for the presence of mutations related to CQ and other antimalarials using a PCR-RFLP assay. Results. There was a wide prevalence of pfcrt gene mutations with the pfcrt 76T CQ resistance marker being predominant (97.7%). The prevalence of other pfcrt mutations varied from high (75E: 88%) to moderate (74I: 79.1%, 220S: 69.8%, 271E and 371I: 53.5%) or low (326S: 36%, 72S: 10.5%). Mutated pfcrt 72–76 amino acids haplotypes were highly prevalent (98.8%). Among these, the CVIET classic, old-world African/Southeast Asian haplotype was the most predominant, and was mostly found in the isolates from the Khamis Bani Saad district of Al-Mahwit (93.1%) and the AdDahi district of Hodeidah (88.9%). However, it was only found in 26.3% of the isolates from the Bajil district of Hodeidah. Surprisingly, the SVMNT new-world South American haplotype was exclusively detected in 9.3% of the isolates from the Bajil district of Hodeidah. Mutations at Y184F of pfmdr1 were found in all isolates (100%) from all districts. The mutation for codons 1034C and 86Y were found only in the isolates from the AdDahi and Khamis Bani Saad districts. Overall, the AdDahi and Khamis Bani Saad districts were similar in terms of carrying most of the mutations in the pfcrt and pfmdr1 genes, while there was a lower prevalence of mutation in the isolates from the Bajil district. Conclusion. The high prevalence of mutations in pfcrt 5 years after the official cessation of CQ use against P. falciparum suggests that there is sustained CQ pressure on P. falciparum isolates in the study area. Moreover, the low prevalence of mutations in the pfmdr1 gene could be a good indicator of the high susceptibility of P. falciparum isolates to antimalarials other than CQ. A new strategy to ensure the complete nationwide withdrawal of CQ from the private drug market is recommended