Industry consortia and network externalities : a cross-sector comparison

Although the prevalence of multi-firm alliances or consortia in many industry sectors has been noted by many researchers, there is limited research on such alliance forms. This study sought to address the following questions: How prevalent are consortia and in which industry sectors are they most prevalent? What are the characteristics of the industries and what are the relationships between firms in such strategic blocks? Our results show that all sectors show a sharp drop in frequency after the threshold of 4 partners in an alliance is reached, suggesting a significant difference between dyadic or triadic alliances and larger-sized alliance forms. However, there is considerable variation across industry sectors in the frequency of alliances with more than two partners, with a range from zero to over 20%. Case studies of selected consortia suggest that network externalities are a significant factor in formation of many of the large consortia. Unexpectedly corporate social responsibility was also identified as a possible factor in other cases

Trends in investor relations : treating investors as customers?

Many companies now treat shareholders as another customer group and apply marketing principles to investor relations; this is broadening the boundaries of traditional marketing thinking. There has been a shift from a transaction approach to a relationship approach in marketing (Gronross, 1994, Vargo & Lusch, 2004) as well as towards a more integrated approach to marketing communication (Schultz & Kitchen, 2000), however investor relations are in many cases still seen as separate from the “mainstream” marketing. This paper looks at the factors in the business environment that are changing the role of investor relations in companies, the various investor relations instruments and the increasing role that the Internet plays in treating investors as customers

Minicircle DNA-mediated endothelial nitric oxide synthase gene transfer enhances angiogenic responses of bone marrow-derived mesenchymal stem cells

BACKGROUND: Non-viral-based gene modification of adult stem cells with endothelial nitric oxide synthase (eNOS) may enhance production of nitric oxide and promote angiogenesis. Nitric oxide (NO) derived from endothelial cells is a pleiotropic diffusible gas with positive effects on maintaining vascular tone and promoting wound healing and angiogenesis. Adult stem cells may enhance angiogenesis through expression of bioactive molecules, and their genetic modification to express eNOS may promote NO production and subsequent cellular responses. METHODS: Rat bone marrow-derived mesenchymal stem cells (rBMSCs) were transfected with a minicircle DNA vector expressing either green fluorescent protein (GFP) or eNOS. Transfected cells were analysed for eNOS expression and NO production and for their ability to form in vitro capillary tubules and cell migration. Transcriptional activity of angiogenesis-associated genes, CD31, VEGF-A, PDGFRα, FGF2, and FGFR2, were analysed by quantitative polymerase chain reaction. RESULTS: Minicircle vectors expressing GFP (MC-GFP) were used to transfect HEK293T cells and rBMSCs, and were compared to a larger parental vector (P-GFP). MC-GFP showed significantly higher transfection in HEK293T cells (55.51 ± 3.3 %) and in rBMSC (18.65 ± 1.05 %) compared to P-GFP in HEK293T cells (43.4 ± 4.9 %) and rBMSC (15.21 ± 0.22 %). MC-eNOS vectors showed higher transfection efficiency (21 ± 3 %) compared to P-eNOS (9 ± 1 %) and also generated higher NO levels. In vitro capillary tubule formation assays showed both MC-eNOS and P-eNOS gene-modified rBMSCs formed longer (14.66 ± 0.55 mm and 13.58 ± 0.68 mm, respectively) and a greater number of tubules (56.33 ± 3.51 and 51 ± 4, respectively) compared to controls, which was reduced with the NOS inhibitor L-NAME. In an in vitro wound healing assay, MC-eNOS transfected cells showed greater migration which was also reversed by L-NAME treatment. Finally, gene expression analysis in MC-eNOS transfected cells showed significant upregulation of the endothelial-specific marker CD31 and enhanced expression of VEGFA and FGF-2 and their corresponding receptors PDGFRα and FGFR2, respectively. CONCLUSIONS: A novel eNOS-expressing minicircle vector can efficiently transfect rBMSCs and produce sufficient NO to enhance in vitro models of capillary formation and cell migration with an accompanying upregulation of CD31, angiogenic growth factor, and receptor gene expression

MLaaS : a cloud system for mobile micro learning in MOOC

Mobile learning in massive open online course (MOOC) differs evidently from its traditional ways as it relies more on collaboration and becomes fragmented. We introduce a cloud-based system which can organize learners into a better teamwork context and customize micro learning resources in order to meet personal demands in real time. Particularly, a smart micro learning environment can be built by a newly designed SaaS, in which educational data mining techniques are mainly employed to understand learners' behaviors and recognize learning resource features

Minicircle DNA-mediated endothelial nitric oxide synthase gene transfer enhances angiogenic responses of bone marrow-derived mesenchymal stem cells

BACKGROUND: Non-viral-based gene modification of adult stem cells with endothelial nitric oxide synthase (eNOS) may enhance production of nitric oxide and promote angiogenesis. Nitric oxide (NO) derived from endothelial cells is a pleiotropic diffusible gas with positive effects on maintaining vascular tone and promoting wound healing and angiogenesis. Adult stem cells may enhance angiogenesis through expression of bioactive molecules, and their genetic modification to express eNOS may promote NO production and subsequent cellular responses. METHODS: Rat bone marrow-derived mesenchymal stem cells (rBMSCs) were transfected with a minicircle DNA vector expressing either green fluorescent protein (GFP) or eNOS. Transfected cells were analysed for eNOS expression and NO production and for their ability to form in vitro capillary tubules and cell migration. Transcriptional activity of angiogenesis-associated genes, CD31, VEGF-A, PDGFRα, FGF2, and FGFR2, were analysed by quantitative polymerase chain reaction. RESULTS: Minicircle vectors expressing GFP (MC-GFP) were used to transfect HEK293T cells and rBMSCs, and were compared to a larger parental vector (P-GFP). MC-GFP showed significantly higher transfection in HEK293T cells (55.51 ± 3.3 %) and in rBMSC (18.65 ± 1.05 %) compared to P-GFP in HEK293T cells (43.4 ± 4.9 %) and rBMSC (15.21 ± 0.22 %). MC-eNOS vectors showed higher transfection efficiency (21 ± 3 %) compared to P-eNOS (9 ± 1 %) and also generated higher NO levels. In vitro capillary tubule formation assays showed both MC-eNOS and P-eNOS gene-modified rBMSCs formed longer (14.66 ± 0.55 mm and 13.58 ± 0.68 mm, respectively) and a greater number of tubules (56.33 ± 3.51 and 51 ± 4, respectively) compared to controls, which was reduced with the NOS inhibitor L-NAME. In an in vitro wound healing assay, MC-eNOS transfected cells showed greater migration which was also reversed by L-NAME treatment. Finally, gene expression analysis in MC-eNOS transfected cells showed significant upregulation of the endothelial-specific marker CD31 and enhanced expression of VEGFA and FGF-2 and their corresponding receptors PDGFRα and FGFR2, respectively. CONCLUSIONS: A novel eNOS-expressing minicircle vector can efficiently transfect rBMSCs and produce sufficient NO to enhance in vitro models of capillary formation and cell migration with an accompanying upregulation of CD31, angiogenic growth factor, and receptor gene expression

Fabrication of transparent YAG ceramics from co-precipitation synthesized nanopowders

YAG nanopowders were synthesized by a co-precipitation method using ammonium hydrocarbonate and ammonia water as the precipitants respectively. The influences of precipitants on chemical compositions, phase transformation and sinterability of the prepared powders, and transmittance of the vacuum-sintered YAG ceramics were studied. The sinterability of powders synthesized using ammonium hydrocarbonate as precipitant is better than that with ammonia water. Pure YAG phase can be obtained by calcining the hydrate precursor at 1200°C, while some impurity phases exist when calcining the carbonate precursor at the same temperature. Transparent YAG ceramics were fabricated by vacuum sintering at 1700°C for 5 h using the YAG nanopowders, and their in-line transmittance is about 60% in the visible light range

Mesenchymal stem cells expressing eNOS and a Cav1 mutant inhibit vascular smooth muscle cell proliferation in a rat model of pulmonary hypertension

Background: This study aimed to investigate the effect of bone marrow derived mesenchymal stem cells (rBMSCs) transduced with lentiviral vectors expressing endothelial nitric oxide synthase (eNOS) and/or a mutant caveolin-1(F92A-Cav1), on the pulmonary haemodynamics and structure in a rat model of pulmonary arterial hypertension (PAH). Methods: Pulmonary arterial hypertension was induced with monocrotaline (MCT) in 60 adult male Wistar rats prior to delivery of lentiviral vector transduced rBMSCs expressing Cav1, eNOS and/or F92A-Cav1. Changes in pulmonary haemodynamics, right ventricular hypertrophy index (RVHI), and serum nitric oxide (NO) were evaluated. Ultrastructure changes in lung tissues were observed by transmission electron microscopy. Expression of Kruppel-like factor 4 (KLF4), p53, P21, eNOS, and alpha-smooth muscle actin were evaluated by real time PCR, western blotting or immunohistochemistry. Results: Treatment of PAH rats with gene modified rBMSCs (eNOS +/- Cav1 F92A) decreased right ventricular systolic pressure and improved pulmonary haemodynamics. The protein of alpha-smooth muscle actin expression was decreased whilst KLF4, p53, P21, eNOS expression, and serum NO concentration was elevated. The survival rate of rats in the treatment groups was also improved, after 35 days of observation. Conclusion: Intravenous delivery of rBMSCs expressing eNOS/F92A-Cav1 to PAH rats inhibits pulmonary vascular smooth muscle cell proliferation, and improves pulmonary haemodynamics, vascular remodelling and short-term survival. Activation of KLF4-p53 signalling pathway may be involved in these beneficial effects

Land Surface Temperature Retrieval from Sentinel-3A Sea and Land Surface Temperature Radiometer, Using a Split-Window Algorithm

Land surface temperature (LST) is a crucial parameter in the interaction between the ground and the atmosphere. The Sentinel-3A Sea and Land Surface Temperature Radiometer (SLSTR) provides global daily coverage of day and night observation in the wavelength range of 0.55 to 12.0 μm. LST retrieved from SLSTR is expected to be widely used in different fields of earth surface monitoring. This study aimed to develop a split-window (SW) algorithm to estimate LST from two-channel thermal infrared (TIR) and one-channel middle infrared (MIR) images of SLSTR observation. On the basis of the conventional SW algorithm, using two TIR channels for the daytime observation, the MIR data, with a higher atmospheric transmittance and a lower sensitivity to land surface emissivity, were further used to develop a modified SW algorithm for the nighttime observation. To improve the retrieval accuracy, the algorithm coefficients were obtained in different subranges, according to the view zenith angle, column water vapor, and brightness temperature. The proposed algorithm can theoretically estimate LST with an error lower than 1 K on average. The algorithm was applied to northern China and southern UK, and the retrieved LST captured the surface features for both daytime and nighttime. Finally, ground validation was conducted over seven sites (four in the USA and three in China). Results showed that LST could be estimated with an error mostly within 1.5 to 2.5 K from the algorithm, and the error of the nighttime algorithm involved with MIR data was about 0.5 K lower than the daytime algorithm