Implante hematopoyético de una serie de pacientes movilizados con Plerixafor. Estudio retrospectivo multicéntrico

Poster [PC-256] Introducción: Plerixafor (PLX) es un inhibidor del receptor CXCR4 de probada eficacia en la recolección de PHSP para autotrasplante hematopoyético (TASPE) en pacientes malos movilizadores. Los estudios llevados a cabo hasta la fecha se han centrado mayoritariamente en aspectos referentes a la cinética de movilización (Mx) y rendimiento. El objetivo de nuestro estudio es recoger una amplia experiencia multicéntrica sobre el injerto hematopoyético a corto y medio plazo de pacientes que habían sido movilizados con PLX. Paciente: s y Métodos: Estudiamos retrospectivamente todos los pacientes que recibieron PLX como parte del esquema de movilización de PHSP para TASPE durante los años 2008-18 en siete hospitales de la zona norte; las enfermedades de base fueron: 94 linfomas no Hogdkin, 14 con enfermedad de Hogdkin, 78 mieloma múltiple y 4 con otros diagnósticos. 108 pacientes eran varones (56%) y 82 mujeres (44%). Su edad mediana era 59 años (4-73). Su peso y su altura oscilaron entre 15-134 kg y 106-188 cm, respectivamente. La mediana de líneas de tratamiento de nuestra serie fue de 2 (1-5); veintiún pacientes habían recibido radioterapia extensa y 15 pacientes uno o varios TASPEs previos. El número de intentos de Mx previos osciló entre 0 y 4 (mediana: 1). La pauta de G-CSF empleada fue de 5-10 mcg/kg/12 h durante 1-14 días (mediana: 6 días) y la dosis de PLX fue la recomendada en ficha técnica (0.24mcg/Kg/24horas). En 36 casos (18, 95%) la movilización se realizó en la fase de recuperación tras un ciclo de la quimioterapia de tratamiento. Tabla 1. Características de la serie y empleo de PLX. Resultados: se realizaron un total de 159 TASPEs. El estatus de la hemopatía en el momento del trasplante era remisión completa en el 50% de los casos. En cuanto a los resultados de movilización y colecta, la mediana de sesiones de aféresis requeridas fue de 2, rango (0-5) y la cifra de células CD34+ recolectada fue de 2, 79 (x 106/kg) con un rango entre 0 y 30, 3. Tabla 2. Datos de implante a corto y medio plazo Conclusiones: 1) el empleo de Plerixafor permitió realizar el TASPE en un alto porcentaje de pacientes malos movilizadores; 2) la calidad del injerto a corto y medio plazo de los pacientes autotrasplantados movilizados con PLX fue óptimo en la gran mayoría de los casos; 3) en nuestro conocimiento, esta serie multicéntrica es una de las mayores comunicadas enfocada en la calidad del implante a corto y medio plazo de pacientes malos movilizadores sometidos a TASPE

Novel Peptide Biomarker Discovery for Detection and Identification of Bacterial Pathogens by LC-ESI-MS/MS

9 páginas, 1 tabla, 7 figuras.-- This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are creditedThe increase of foodborne diseases in the past years, especially emergent pathogens is a great concern for authorities, industry and consumers. It is important the early detection and identification of the microbiota in order to evaluate the safety of the product and avoid health issues and economic losses for either the producer or the distributor. Consequently, there is a critical need to develop rapid and sensitive methods for detection and accurate identification of food-borne pathogens. Our approach is the description of peptide/protein markers unique for a bacterial species, strain or genera. The identification of bacterial peptide/protein markers was performed after protein extraction, in-solution tryptic digestion and analysizing its tryptic peptides by liquid chromatographyelectrospray ionization-tandem mass spectrometry (LC-ESI-MS/MS). Subsequent analysis with database search software and comparison with amino acid sequences available in public databases (Uniprot and NCBI) allowed the identification of several peptide biomarkers. In this study we have focused on two proteins of the species Staphylococcus aureus, and compared them with the public databases and with the data obtained from LC-ESI-MS/MS of the trysinized proteins from Gram-positive and Gram-negative bacteria such as Streptococcus spp., Enterococcus spp., Bacillus spp., Listeria spp, Salmonella spp., Enterobacteriaceae. As a result, cysteine synthase and cysteine proteases stophapain A and stophapain B, and 1-pyrroline-5-carboxylate dehydrogenase proteins may be used for S. aureus differentiation. Furthermore, cysteine protease peptides AQKPVDNITQIIGGTPVVK, TESIPTGNNVTQLK, MTTYNEVDNLTK, YTINVSSFLSK and KTGSPDYLLHFLEQKV resulted specific for S. aureus, being potentially useful for a fast detection of bacterial pathogens directly in food products and not requiring previous culture of the bacteriaThis work was funded by the Spanish Ministry of Economy and Competitivity Project AGL2013-48244-R and by the European Regional Development Fund (ERDF) (2007-2013). The work of Sonia Caamaño Antelo is supported by the Otilia Millares FoundationPeer reviewe

Biomarker discovery for foodborne pathogen detection by LC-MS/MS

5 pages, 3 figures, 2 tablesFor an effective food control and monitoring of foodborne pathogens, methods are required that are able to detect rapidly, precisely and quantitatively microbial contaminants in complex mixtures and directly in the food matrices. In the present study, we focused on this aim and searched for specific peptide biomarkers for the main histamine-producing bacterial species, potentially present in fish and seafood products. Such biomarkers could be targets for the identification of the corresponding species at low concentrations in a complex food matrix. The determination of possible candidate peptide biomarkers was carried out by ESI-LC-MS/MS analysis of the tryptic digests of the bacterial protein extracts. Previously, specific peaks detected by MALDI-TOF MS analysis were identified by database searches. The focus of the study was then given to those proteins and corresponding peptides that have been determined as specific by bioinformatics tools, such as BLAST and alignment of the protein sequences. Finally, the determined specific peptides have been studied in relation to their abundance, spectral quality and reproducibility, with the aim to evaluate their potential as biomarkers for bacterial identification in complex mixturesThis work was funded by the Project AGL2013- 48244-R from the Spanish Ministry of Economy and Competitiveness and by the European Regional Development Fund (ERDF) (2007-2013). The work of S. Caamaño Antelo is supported by the Otilia Millares FoundationN

Bacterial identification by LC-ESI-IT-MS/MS

5 pages, 3 figuresThe increase of foodborne diseases in the past years, especially emergent pathogens is a great concern for authorities, industry and consumers. It is important the early detection and identification of the microbiota in order to evaluate the safety of the product and avoid health issues and economic losses for either the producer or the distributor. Consequently, there is a critical need to develop rapid and sensitive methods for detection and accurate identification of food-borne pathogens. Our approach is the description of peptide/protein markers unique for a bacterial species, strain or genera. The identification of bacterial peptide/protein markers was performed after digesting the protein in solution and analyzing its tryptic peptides by liquid chromatography-electrospray ionization-tandem mass spectrometry (LC-ESI-MS/MS), and subsequent analysis with database search software and comparison with amino acid sequences available in public databases (NCBI). Two peptides were found to be potentially useful for the detection of Staphylococcus aureusThis work was funded by the Spanish Ministry of Economy and Competitivity Project AGL2013-48244-R and by the European Regional Development Fund (ERDF) (2007-2013). The work of Sonia Caamaño Antelo is supported by the Otilia Millares FoundationN