A Novel Fibrinolytic Enzyme from Bacillus Sphaericus MTCC 3672: Optimization and Purification Studies

A novel extracellular fibrinolytic enzyme was produced from Bacillus sphaericus MTCC 3672 for dissolving blood clots. Optimized fermentation parameters achieved by one factor at a time approach had demonstrated 2.85 fold increase in fibrinolytic activity i.e. from 3.5*104 U/l (basal media) to 9.98*104 U/l after 24 h of incubation in submerged fermentation. Statistical screening of six independent nutritional variables such as, glucose, yeast extract, NaCl, MgCl2, MnCl2, CaCl2 was studied using Plackett-Burman design. Amongst six variables, yeast extract was found to be significant factor affecting yield of a fibrinolytic enzyme. Furthermore, growth kinetics of biomass formation, enzyme production, and substrate utilization was evaluated by unstructured kinetic models and various biokinetic parameters such as µmax (0.37 1/h), Pr (0.12 1/h), YP/S (7.74 U/g) and YX/S (3.32 g/g) were determined. In purification step, ultrafiltered broth was purified with DEAE Cellulose anion exchange chromatography and Sephadex G100 gel filtration chromatography with 10 fold purity. In vitro fibrin clot degradation study had revealed significant breakdown of fibrin clot. The fibrinolytic activity of purified enzyme (7.5 mm) was found to be matching with marketed Nattolife® (8.0 mm) (nattokinase) and superior to Thromboflux® (0 mm) (streptokinase) formulations. Hence, efficient formulation containing purified fibrinolytic agent can be used for medical emergencies viz. myocardial infarction and deep vein thrombosis

Slicing: A New Access to Isolation Preserving Data Publishing

There are several anonymizing techniques like Abstraction, Containerization for isolation preserving small data publishing. The Abstraction loses amount of information for high spatial data. Containerization does not avoid enrollment acknowledgment and does not give clear separation between aspects. We are presenting a technique called slicing which partitions the data both horizontally and vertically. We also show that slicing conserves better data service than abstraction and can be used for enrollment acknowledgment conservation. One more important advantage of slicing is that it can handle high-spatial data. Slicing can be used for aspect acknowledgment conservation and establishing an efficient algorithm for computing the sliced data that obey the l-diversity concern. Our experiments confirm that slicing conserves better service than abstraction and is more active than containerization in loads affecting the conscious aspect and also demonstrate that slicing can be used to avoid enrollment acknowledgment. DOI: 10.17762/ijritcc2321-8169.16045

“In Vitro study of Antibacterial properties of GokshurKwatha in Urinary Tract Infection by Culture and Sensitivity Method.”

The study was planned to evaluate the anti bacterial property of GokshurKwatha onUrinary Tract Infection by culture & sensitivity method for the study,30 patients were selected & urine sample used for culture by stricking method & colony characters are observed & gram staining done. Then specially made Gokshurkwath discs were used for sensitivity in MH agar. Zone of inhibition is the criteria of assessment of sensitivity. Whole study was in vitro. The bacterias during study are Escherichia Colii, KlebsiellaPnuemonii,Pseudomonas. It observed that Gokshurkwath shows significant activity against above organism

Enhanced Searchable Public Key Cipher Text With Hidden Structures For Fast Keyword Search

Existing semantically secure public-key searchable coding schemes take search time linear with the overall variety of the cipher texts. This makes retrieval from large-scale databases preventative. To alleviate this drawback, this paper proposes Searchable Public-Key Cipher texts with Hidden Structures (SPCHS) for keyword search as quick as potential while not sacrificing linguistics security of the encrypted keywords. In SPCHS, all keyword-searchable Cipher texts area unit structured by hidden relations, and with the search trapdoor such as a keyword, the minimum info of the relations is disclosed to an enquiry rule because the steering to search out all matching Cipher texts expeditiously. We have a tendency to construct a SPCHS theme from scratch during which the Cipher texts have a hidden star-like structure. We have a tendency to prove our theme to be semantically secure within the Random Oracle (RO) model. The search quality of our theme relies on the particular variety of the Cipher texts containing the queried keyword, instead of the amount of all Cipher texts. Finally, we have a tendency to gift a generic SPCHS construction from anonymous identity-based coding and collision-free full-identity malleable Identity-Based Key Encapsulation Mechanism (IBKEM) with namelessness. We have a tendency to illustrate 2 collision-free full-identity malleable IBKEM instances, that area unit semantically secure and anonymous, severally, within the artificial language and customary models

Study on Morbidity and Mortality Rates in Buffaloes in Pune Division of Maharashtra State in India

The present study was carried out to analyse morbidity and mortality rate in buffaloes and its associated factors in Pune division of Maharashtra state in India. Stratified two stages random sampling design was adopted & the data of total 564 buffaloes were collected through pre-tested modified schedule from 157 buffalo owners. The data were analysed statistically by SAS 9.3 software for evaluation of Chi-square and Logistic regression analysis. Overall morbidity (28.01%) and mortality (7.98%) rates were recorded in study area. Digestive diseases and respiratory diseases are major cause of the higher morbidity and mortality in buffaloes, respectively. Statistically, there wasn't significant association of overall disease incidence with age or sex. However, the Chi-square analysis of overall mortality rate showed significance (p<0.01) difference among age and sex. Logistic regression analysis also suggested the same results. Mortality rates were recorded higher in calves and male buffaloes as compared to their respective counterparts. It is suggested that digestive and respiratory problems may be reduced by improving feeding and management practices. This study provides the important tool for determining the health status of buffaloes and has special importance in planning of prevention and control strategies designed to reduce the incidences of diseases in livestock and therefore economic status of farmers

Evaluation of sampling gear for demersal resource surveys

The three demersal trawls evaluated were 38 m HSDT-II, indigenously developed by CIFT for deep sea fishing in Indian EEZ; and two imported designs, viz., 45.6 m Expo model demersal trawl and 50 m fish trawl operated from vessels of FSI and IFP, respectively. Vertical opening at trawl mouth was heighest for 50 m fish trawl (3.2 m), followed by Expo model demersal trawl (2.5 m) and 38 m HSDT-II (2.2 m), due to differences in overall dimensions and design features. Estimate of horizontal opening between otter boards was highest for 38 m HSDT-II probably due to low drag of the gear, followed by 45.6 m and 50 m trawls. Lowest catch per unit effort obtained by 38 m HSDT-II is presumably due to smaller dimensions of the gear, larger codend mesh size and difference in ground rig, in addition to chance factors. However, 38 m HSDT-II scores on several features desirable in demersal sampling gear such as simplicity in design and construction, ease of operation; lower twine surface area and drag; and ground rig suitable for wider range of bottom conditions. Modifications to make it more effective while sampling for crustaceans and small sized finfish components are described

Optimization of production, biochemical characterization and In Vitro evaluation of the therapeutic potential of fibrinolytic enzymes from a new Bacillus Amyloliquefaciens

The capacity of fibrinolytic enzymes to degrade blood clots makes them of high relevance in medicine and in the pharmaceutical industry. In this work, forty-three microorganisms of the genus Bacillus were evaluated for their potential to produce fibrinolytic proteases. Thirty bacteria were confirmed as producers of fibrinolytic enzymes, the best results obtained for the strain Bacillus amyloliquefaciens UFPEDA 485. The optimization of the enzyme production conditions was done by a central composite design (CCD) star 23 that allowed to define the optimal conditions for soybean flour and glucose concentrations and agitation rate. The highest fibrinolytic activity (FA) of 813 U mL-1 and a degradation of blood clot in vitro of 62% were obtained in a medium with 2% (w/v) of soybean flour and 1% (w/v) glucose at 200 rpm after 48 h of cultivation, at pH 7.2 and 37 °C. The obtained fibrinolytic enzyme was characterized biochemically. Fibrinolytic activity was inhibited by PMSF (fluoride methylphenylsulfonyl - C7H7FO2S) 91.52% and EDTA (ethylenediaminetetraacetic acid - C10H16N2O8) 89.4%, confirming to be a serine- metallo protease. The optimum pH and temperature were 7.0 and 37 oC, respectively, and the enzyme was stable for 12 h. The fibrinolytic activity at physiological conditions of this enzyme produced by Bacillus amyloliquefaciens UFPEDA 485, as well as its long term stability, demonstrate that it has suitable characteristics for human and veterinary applications, and promises to be a powerful drug for the treatment of vascular diseases.We express our thanks to Coordination for the Improvement of Higher Level Education Personnel (CAPES) - Doctoral Sandwich Program (PDSE) Nº 0259/ 12-8 and National Council for Scientific and Technological Development (CNPq) - Nº 202026/2011-6 for the financial support

Ultrasonic intensification as a tool for enhanced microbial biofuel yields

peer-reviewedUltrasonication has recently received attention as a novel bioprocessing tool for process intensification in many areas of downstream processing. Ultrasonic intensification (periodic ultrasonic treatment during the fermentation process) can result in a more effective homogenization of biomass and faster energy and mass transfer to biomass over short time periods which can result in enhanced microbial growth. Ultrasonic intensification can allow the rapid selective extraction of specific biomass components and can enhance product yields which can be of economic benefit. This review focuses on the role of ultrasonication in the extraction and yield enhancement of compounds from various microbial sources, specifically algal and cyanobacterial biomass with a focus on the production of biofuels. The operating principles associated with the process of ultrasonication and the influence of various operating conditions including ultrasonic frequency, power intensity, ultrasonic duration, reactor designs and kinetics applied for ultrasonic intensification are also described

RP-HPLC analytical method development and validation of obeticholic acid in bulk and marketed formulation

The purpose of this study is to develop a new analytical RP-HPLC method for quantifying Obeticholic Acid in bulk and commercial formulations. Liquid chromatography is performed on a C18 column and the mobile phase is prepared using 0.1% orthophosphoric acid and acetonitrile in a ratio of 65:35. The flow rate was 1.0 ml / min and the injection volume was 10 ml. The maximum absorption of the drug was 210 nm. The retention time was found to be 2.80 minutes. This method was found to be accurate and linear with a correlation coefficient of 0.999 in the range 2.4-14 mg / mL. The percentage RSD for accuracy was less than 1.8%, averaging 100.05%. All validation parameters were statistically validated according to ICH guidelines and were within acceptable criteria. This method turned out to be simpler, more accurate, more specific, more accurate and more robust. This HPLC method is used for the analysis of obeticholic acid