A new member of the LIR family from perennial ryegrass is cold-responsive, and promotes vegetative growth in ¤Arabidopsis¤

A cold-regulated gene Lolium perenne LIR1 (LpLIR1) was isolated from perennial ryegrass using a subtractive approach. The gene has strong homology to the Light Induced Rice1 (LIR1) gene and is regulated at the transcriptional level by cold, and by a diurnal rhythm. Expression of LpLIR1 in perennial ryegrass was upregulated by vernalization but did not follow a standard vernalization-responsive expression pattern. LpLIR1 expression was restricted to vegetative tissues and absent in apices during floral induction and in flowers. LpLIR1 mRNA levels displayed diurnal fluctuations, which peaked before dusk and declined during the night. Heterologous expression of LpLIR1 in Arabidopsis led to a significant increase in leaf formation under short days (SD) conditions but only when plants had received a preceding vernalization treatment. Furthermore, dissection of plant development under SD revealed a minor but significant delay of flowering in the transgenic lines compared to wildtype plant

Association of the intraoperative peripheral perfusion index with postoperative morbidity and mortality in acute surgical patients: a retrospective observational multicentre cohort study

BACKGROUND: We hypothesised that in acute high-risk surgical patients, a lower intraoperative peripheral perfusion index (PPI) would indicate a higher risk of postoperative complications and mortality. METHODS: This retrospective observational study included 1338 acute high-risk surgical patients from November 2017 until October 2018 at two University Hospitals in Denmark. Intraoperative PPI was the primary exposure variable and the primary outcome was severe postoperative complications defined as a Clavien–Dindo Class ≥III or death, within 30 days. RESULTS: intraoperative PPI was associated with severe postoperative complications or death: odds ratio (OR) 1.12 (95% confidence interval [CI] 1.05–1.19; P0.5, P=0.003. If PPI was ≤1.5, 30-day mortality was 16% vs 8% in patients with a PPI >1.5 (P<0.001). In contrast, intraoperative mean MAP ≤65 mm Hg was not significantly associated with severe postoperative complications or death (OR 1.21 [95% CI 0.92–1.58; P=0.2]). CONCLUSIONS: Low intraoperative PPI was associated with severe postoperative complications or death in acute high-risk surgical patients. To guide intraoperative haemodynamic management, the PPI should be further investigated