Bioinformatics Facilitate Haplotype Analysis in Psoriasis

Psoriasis is an inflammatory skin disease. Vitamin D is successfully used for psoriasis therapy. Polymorphisms and haplotypes in the VDR gene may explain the differences in response to vitamin D therapy. We study vitamin d receptor gene polymorphisms in 100 psoriasis patients and 100 control subjects. We have detected SNPs by PCR-RFLP and analyze by CHAPLIN. We found statistically significant allele genotype and haplotype in patients

Vitamin D receptor gene polymorphisms and haplotypes (Apa I, Bsm I, Fok I, Taq I) in Turkish psoriasis patients.

BACKGROUND: Psoriasis is an inflammatory disease characterized by increased squamous cell proliferation and impaired differentiation. Vitamin D, Calcitriol, and its analogues are successfully used for psoriasis therapy. However, it is unknown why some psoriasis patients are resistant to Vitamin D therapy. Vitamin D mediates its activity by a nuclear receptor. It is suggested that polymorphisms and haplotypes in the VDR gene may explain the differences in response to vitamin D therapy. MATERIAL/METHODS: In this study, 102 psoriasis patients and 102 healthy controls were studied for VDR gene polymorphisms. The Fok I, Bsm I, Apa I and Taq I polymorphisms were examined by PCR-RFLP, and 50 subjects received vitamin D therapy to evaluate the association between VDR gene polymorphisms and response to vitamin D therapy. Existence of cutting site is shown by capital letters, and lack was shown by lower case. The haplotypes were analysed by CHAPLIN. RESULTS: There was significant difference in allele frequency of T and genotype frequency of Tt between cases and controls (p values 0.038 and 0.04, respectively). The Aa and bb genotypes were significantly higher in early onset than late onset psoriasis (p values 0.008 and 0.04, respectively). The genotypes Ff, ff and TT are significantly different between vitamin D3 therapy responders and non-responders (p values 0.04, 0.0001, 0.009, respectively). To the best of our knowledge, this is the first report showing importance of VDR gene haplotypes in psoriasis, the significance of the Wald and LR (Likelihood Ratio) statistics (p=0,0042) suggest that FfBbAatt is a disease-susceptibility haplotype. CONCLUSIONS: Haplotype analysis is a recent and commonly used method in genetic association studies. Our results reveal a previously unidentified susceptibility haplotype and indicate that certain haplotypes are important in the resistance to vitamin D3 therapy and the onset of psoriasis. The haplotypes can give valuable data where genotypes unable to do

Optical and Vapor Sensing Properties of Calix[4]arene Langmuir-Blodgett Thin Films with Host–Guest Principles

25,27-(Dipropylmorpholinoacetamido)-26,28-dihydroxycalix[4]arene was used as a chemical sensor material in this work. The calix[4]arene LB thin films were prepared onto a gold-coated glass and quartz glass substrates to fabricate a thin film chemical sensor element. Atomic Force Microscopy (AFM) and Surface Plasmon Resonance (SPR) techniques were used to characterize all the calix[4]arene LB thin films. The film thickness and the refractive index of thin films can be evaluated with the fitted experimental SPR datas. The refractive index and the thickness per monolayer of LB films were determined as a 1.58 ± 0.04 and 1.27 ± 0.09 nm, respectively. The calix[4]arene LB thin film chemical sensor element was exposed to dichloromethane, chloroform, benzene and toluene vapors. The SPR kinetic measurements displayed that, the photodetector response change, ?Irf for saturated dichloromethane vapor is much larger than the other vapors with the ?Irf value of 48 au and the diffusion coefficient value of 5.1 × 10?16 cm2s?1. Swelling process was analyzed by well known Fick's Equations. In this approach diffusion coefficients (D) for swelling were conformed to the square root of time and were correlated with the volatile organic compounds. Our results showed that calix[4]arene thin film has a highly selective with a large response to dichloromethane vapor. © 2018, © 2018 Taylor & Francis Group, LLC.UBAP 2017/HD-MF001This work was supported by the Research Foundation of Usak University (UBAP 2017/HD-MF001). We also thank Usak University Scientific Analysis Technological Application and Research Center (UBATAM) for their support

Organic vapor sensing properties and characterization of ?-naphthylmethacrylate LB thin films

Determination of organic vapor sensing properties of ?-Naphthylmethacrylate (?-NMA) monomer based Langmuir-Blodgett (LB) thin films was aimed in this study. LB thin film fabrication was performed on quartz glass and quartz crystal substrates in order to investigate the characterization and organic vapor properties of ?-NMA materials by using UV-Visible, Atomic Force Microscopy (AFM) and Quartz Crystal Microbalance (QCM) techniques. ?-A isotherm graph was taken and a suitable surface pressure value were primarily determined as 13 mN m?1 for successful ?-NMA LB thin film fabrication. Transfer ratio value was found to be ? 0.93 for quartz glass and quartz crystal substrates. The typical frequency shift per layer was obtained as 16.93 Hz/layer and the deposited mass onto a quartz crystal was calculated as 271.30 ng/layer (1.02 ng mm?2). The sensing responses of ?-NMA LB films against dichloromethane, chloroform, toluene and m-xylene were measured by QCM system. Dichloromethane created the maximum shift in the resonance frequency than other organic vapors used in this study. Results exhibited that ?-NMA LB thin films were potential candidates for organic vapor sensing applications, especially high sensitive detection of dichloromethane at room temperature. © 2019, © 2019 Taylor & Francis Group, LLC.216Z080The authors acknowledge The Scientific and Technical Research Council of Turkey (TUBITAK) for its financial support (Project No. 216Z080)

A novel triazine?bearing calix[4]arene: Design, synthesis and gas sensing affinity for volatile organic compounds

A novel triazine-calix[4]arene conjugate was designed and synthesized with the aim to study gas sensing against volatile organic compounds (VOCs) such as dichloromethane, chloroform and carbon tetrachloride. This novel compound was fully characterized by spectroscopic techniques such as FTIR, 1 H and 13 C NMR along with HRMS and BET analysis. The triazine based calix[4]arene organic materials were fabricated onto quartz glasses and quartz crystal substrates to form a thin film chemical sensor element by using Langmuir-Blodgett (LB) technique. Quartz Crystal Microbalance, UV–Visible Spectroscopy, Atomic Force Microscopy and Scanning Electron Microscopy techniques were employed to characterize all these LB thin film layers. Fick's Equations were used for analyzing the swelling process of LB thin film sensor and diffusion coefficient values of organic vapours for swelling were obtained. The initial experiments have revealed that new triazine appended calix[4]arene derivative exhibited an effective chemical gas sensor characteristic with a large response to dichloromethane vapour. © 2019 Elsevier Ltd2018/MF002The financial support from the Research Foundation of Usak University (Project No: 2018/MF002 ) is gratefully acknowledged. Additionally, we thank UBATAM (Usak University, Scientific Analysis Technological Application and Research Center) for analyses. Appendix

Chemical Sensor Properties and Mathematical Modeling of Graphene Oxide Langmuir-Blodgett Thin Films

Graphene oxide (GO) Langmuir-Blodgett (LB) thin films were prepared to use as chemical sensor element. The properties of GO LB thin film layers were characterized via Quartz crystal microbalance (QCM) method. Selectivity of the sensor to various organic vapors was investigated by surface plasmon resonance (SPR) and QCM techniques. Fick's equations were used for calculating the diffusion coefficients (D) values of organic vapors. It was observed that chemical gas sensing characteristics of the GO film sensor shows a large response to some hazardous organic vapors. Then, by using experimental measurements that obtained during this process, NARX-ANN-based mathematical model of frequency shift of the quartz resonator was designed. The results of NARX-ANN verify the efficiency of the designed model. © 2001-2012 IEEE.2018/MF002 British Association for Psychopharmacology, BAPManuscript received April 12, 2019; revised June 19, 2019; accepted June 29, 2019. Date of publication July 2, 2019; date of current version September 18, 2019. This work was supported in part by the BAP: Research Foundation of Usak University under Project 2018/MF002 and in part by the UBATAM: Usak University, Scientific Analysis Technological Application and Research Center. The associate editor coordinating the review of this paper and approving it for publication was Dr. Chang-Soo Kim. (Corresponding author: Yaser Acikbas.) K. Büyükkabasakal is with the Department of Electric-Electronic Engineering, Faculty of Engineering, Usak University, 64200 Us¸ak, Turkey (e-mail: [email protected])

Fabrication and characterization of calix[4]arene Langmuir–Blodgett thin film for gas sensing applications

In this work, the Langmuir–Blodgett (LB) technique was applied onto the different substrates such as quartz, quartz crystals and gold coated glass for fabricating LB thin films with 25,27-(Dipropylmorpholinoacetamido)-26,28-dihydroxycalix[4]arene. The characteristics of the calix[4]arene LB films were evaluated by UV–Vis, atomic force microscopy (AFM), quartz crystal microbalance (QCM) and surface plasmon resonance (SPR) measurements. The mass deposited on the quartz crystal per bilayer was predicted as 2028.80 ng (7.65 ng mm?2) for the calix[4]arene LB film. The sensing abilities of this LB film towards the development of room temperature organic vapor sensing devices were also examined. QCM and SPR systems were fixed to investigate vapor sensing performance of macrocyclic LB films during exposure to volatile organic compounds (VOCs). The macrocyclic LB thin films were more sensitive to dichloromethane than other vapors. The sensitivity and detection limit values of the calix[4]arene QCM sensor to dichloromethane vapor were calculated as 2.023 Hz ppm?1 and 1.482 ppm, respectively. The sensitivities of the calix[4]arene LB films against organic vapors can be explained with the host–guest interaction between the cavity of molecule and the organic vapor molecules. It is determined that the calix[4]arene LB film is more sensitive to dichloromethane than other vapors at room temperature and the calix[4]arene is a promising material serving as a vapor sensing device. © 2017, Springer Science+Business Media B.V.UBAP 2017/HD-MF001Acknowledgements This work was supported by the Research Foundation of Usak University (UBAP 2017/HD-MF001). We also thank Usak University Scientific Analysis Technological Application and Research Center (UBATAM) for their support