Phytochemical Characterization and Antiplatelet Activity of Mexican Red Wines and Their By-products

Red wines and their grape pomaces are important sources of phenolic compounds. Inhibition of plateletaggregation is one of the mechanisms proposed for cardioprotective effect of phenolic compounds fromwine and grape pomace; however, phenolic content is affected by region, variety and winemaking process.In the present study, antiplatelet effect of red wines and grape pomaces was related to its phenolic content(determined by spectrophotometric techniques) and profile (determined using HPLC-MS/MS). in vitroAnti-platelet aggregation was determined using human platelets. Results showed that Zinfandel wine andCabernet Sauvignon grape pomace presented the highest phenolic content. Phenolic profiles presenteddifferences in the presence of flavonoids and oligomeric tannins. Results from platelet aggregationshowed that Merlot and Petit Verdot wines and Petit Verdot grape pomace sample presented the highestantiaggregant effect. These results indicate that antiplatelet effect could be related to phenolic profile thanphenolic content in wines and grape pomaces. Cardioprotective effect of red wines and grape pomacecould be related to specific compounds such as monomeric and polymeric flavan-3-ols

COMPUESTOS BIOACTIVOS Y PROPIEDADES SALUDABLES DEL TAMARINDO (Tamarindus indica L)/ BIOACTIVE COMPOUNDS AND HEALTH PROPERTIES OF TAMARIND (Tamarindus indica L)

El tamarindo (Tamarindus Indica L.) es un fruto procedente de África, cuya producción a nivel mundial se encuentra entre 400 y 500 mil ton, mientras que México produce alrededor de 39 mil ton anuales; este fruto es reconocido en la tradición popular por sus propiedades antioxidantes, posiblemente relacionadas con la presencia de compuestos bioactivos, principalmente compuestos polifenólicos. Al fruto de tamarindo se le han brindado distintas aplicaciones, desde medicinales hasta de ingrediente en la industria alimentaria. La presente revisión tiene la finalidad de recopilar y mostrar algunos de los estudios recientes de los efectos benéficos que se han reportado para la pulpa de este fruto. En los últimos tiempos se han acumulado evidencias de que algunos compuestos polifenólicos ingeridos con la dieta habitual pueden encontrarse asociados con la fibra dietética, por lo que en este trabajo se incluye la cuantificación de los compuestos polifenólicos y la actividad antioxidante en la pulpa del fruto, además de la cuantificación de los compuestos fenólicos que se encuentran potencialmente asociados a la fibra dietética del fruto. Esta evaluación fue realizada por medio de métodos in vitro. Esta sencilla pero novedosa revisión podrá brindar información adicional a la literatura que se encuentra disponible respecto a este fruto, el cual contiene fitoquímicos con bioactividad/ ABSTRACT Tamarind (Tamarindus indica L.) is a fruit from Africa with a worldwide production between 400 and 500 thousand ton, with 39 000 ton produced in Mexico; traditionally it is recognized for its antioxidant properties, possibly related to the presence of bioactive compounds, mainly polyphenols. Tamarind fruit has been given different applications from medicinal as well as an ingredient in the food industry. The present review aims to collect and display some of the recent studies of the beneficial effects that have been reported for the pulp of this fruit. However, in recent times there has been enough evidence which show that some polyphenols ingested with the daily diet may be associated with dietary fiber, so in this review the quantification of polyphenols and antioxidant activity in the fruit pulp was included in addition to the quantification of the phenolic compounds that are potentially associated with dietary fiber of the fruit. This evaluation was performed by in vitro methods. This simple but novel information may provide additional information to the literature that is available around the fruit, which contains phytochemicals with bioactivity

Optimization of total anthocyanin content and antioxidant activity of a Hibiscus sabdariffa infusion using response surface methodology

Hibiscus sabdariffa L. calyces are underutilized sources of health-promoting anthocyanins. Infusions are the most common way to consume them, but because anthocyanins are thermosensitive, prolonged extraction times at high temperatures may reduce their bioactivities, suggesting the need to identify optimal preparation conditions. Response surface methodology was used to establish calyces-to-water ratio (X1: 1–20 g/100 mL), temperature (X2: 70–100 °C), and time (X3: 1–30 min) that would produce an infusion with optimized total anthocyanin content (TAC) and antioxidant activity. Under optimum conditions (X1=10 g/100 mL, X2=88.7 °C, and X3=15.5 min) TAC was 132.7±7.8 mg cyanidin-3-glucoside equivalents (C3G)/100 mL, and antioxidant activity was 800.6±69.9 (DPPH assay), and 1792.0±153.5 (ABTS assay) μmol Trolox equivalents (TE)/100 mL. Predicted and experimental results were statistically similar. Identifying ideal processing conditions can promote consumption of an H. sabdariffa-based functional beverage with high anthocyanin content and antioxidant activity that exert health-promoting bioactivities on the consumer

Changes in Intestinal Microbiota and Predicted Metabolic Pathways During Colonic Fermentation of Mango (Mangifera indica L.)—Based Bar Indigestible Fraction

Mango (Mangifera indica L.) peel and pulp are a source of dietary fiber (DF) and phenolic compounds (PCs) that constituent part of the indigestible fraction (IF). This fraction reaches the colon and acts as a carbon and energy source for intestinal microbiota. The effect of mango IF on intestinal microbiota during colonic fermentation is unknown. In this study, the isolated IF of a novel ‘Ataulfo’ mango-based bar (snack) UV-C irradiated and non irradiated (UVMangoBand MangoB) were fermented. Colonic fermentation occurred in vitro under chemical-enzymatic, semi-anaerobic, batch culture and controlled pH colonic conditions. Changes in the structure of fecal microbiota were analyzed by 16s rRNA gene Illumina MiSeq sequencing. The community´s functional capabilities were determined in silico. The MangoB and UVMangoB increased the presence of Faecalibacterium, Roseburia, Eubacterium, Fusicatenibacter, Holdemanella, Catenibacterium, Phascolarctobacterium, Buttiauxella, Bifidobacterium, Collinsella, Prevotella and Bacteroides genera. The alpha indexes showed a decrease in microbial diversity after 6 h of colonic fermentation. The coordinates analysis indicated any differences between irradiated and non-irradiated bar. The metabolic prediction demonstrated that MangoB and UVMangoB increase the microbiota carbohydrate metabolism pathway. This study suggests that IF of mango-based bar induced beneficial changes on microbial ecology and metabolic pathway that could be promissory to prevention or treatment of metabolic dysbiosis. However, in vivo interventions are necessary to confirm the interactions between microbiota modulating and intestinal beneficial effects

Chemical Composition, Starch Digestibility and Antioxidant Capacity of Tortilla Made with a Blend of Quality Protein Maize and Black Bean

Tortilla and beans are the basic components in the diet of people in the urban and rural areas of Mexico. Quality protein maize is suggested for tortilla preparation because it presents an increase in lysine and tryptophan levels. Beans contain important amounts of dietary fiber. The objective of this study was to prepare tortilla with bean and assesses the chemical composition, starch digestibility and antioxidant capacity using a quality protein maize variety. Tortilla with bean had higher protein, ash, dietary fiber and resistant starch content, and lower digestible starch than control tortilla. The hydrolysis rate (60 to 50%) and the predicted glycemic index (88 to 80) of tortilla decreased with the addition of bean in the blend. Extractable polyphenols and proanthocyanidins were higher in the tortilla with bean than control tortilla. This pattern produced higher antioxidant capacity of tortilla with bean (17.6 μmol Trolox eq/g) than control tortilla (7.8 μmol Trolox eq/g). The addition of bean to tortilla modified the starch digestibility and antioxidant characteristics of tortilla, obtaining a product with nutraceutical characteristics