53 research outputs found
Microscopía electrónica con electrones secundarios y retrodispersos en el esmalte del diente fluorótico
Introducción: El objetivo del presente trabajo consistió en definir los patrones morfológicos del esmalte en
dientes fluoróticos mediante la utilización de la Microscopía Electrónica de Barrido con electrones
secundarios y retrodispersos.
Material y Métodos: Se estudiaron 20 piezas dentarias permanentes de pacientes con fluorosis dental y 5
dientes controles. Todas las muestras fueron procesadas para su estudio morfológico.
Resultados: Se distinguen tres zonas en dientes fluoróticos: zona externa, zona subsuperficial o lesión
fluorótica y zona interna. La zona subsuperficial presenta un triple patrón morfológico característico.
Discusión: Este estudio permite sistematizar patrones estructurales que en un futuro pueden constituir una
base para el desarrollo de futuras estrategias reparativas en dientes fluoróticos.Introduction: The aim of the present study was to establish histological patterns of fluorotic teeth by
scanning electron microscopy (SEM) using secondary and retrodispersive electrons.
Material and Methods: We studied 20 permanent teeth belonging to pacients whith dental fluorosis and 5
control teeth. All samples were processed for morphological study and scanning electron microscopy
(SEM).
Results: We distinguished three areas in fluorotic teeth: external area, subsuperficial area (or fluorotic
lesion) and internal area. Subsuperficial area showed three different types of morphological patterns, which
were characteristic of fluorotic lesions.
Discussion: This study provides systematic structural patterns which in the future can provide a basis for
developing future strategies reparative in fluorotic teeth
Mineralization of human premolar occlusal fissures: a quantitative histochemical microanalysis
The mechanisms of cariogenesis in occlusal
fissures remain elusive because of limited information
about fissure structure and wall mineralization. The
purpose of the present study was to determine the
correlation between morphological patterns in occlusal
fissures in human premolars and quantitative
histochemical patterns of mineralization in the walls of
these formations. We used scanning electron microscopy
and quantitative X-ray microanalysis with the peak-tolocal
background ratio method and microcrystalline
calcium salts as standards. We distinguished three
morphological patterns of fissures in scanning electron
microscopic images. The wall of the fissures was less
mineralized than the control enamel in all three types of
fissures. Because the fissure walls are hypomineralized,
we suggest that practicing dentists should take into
account the degree of mineralization when they are
preparing the fissures for the application of sealant.This work was partially supported by the Ministerio de Educación y Cultura (PB97-0840) and the Agencia Española de Cooperación Internacional (AECI)
Biofabrication of a Tubular Model of Human Urothelial Mucosa Using Human Wharton Jelly Mesenchymal Stromal Cells
Several models of bioartificial human urothelial mucosa (UM) have been described recently.
In this study, we generated novel tubularized UM substitutes using alternative sources of cells.
Nanostructured fibrin–agarose biomaterials containing fibroblasts isolated from the human ureter
were used as stroma substitutes. Then, human Wharton jelly mesenchymal stromal cells (HWJSC)
were used to generate an epithelial-like layer on top. Three differentiation media were used for 7
and 14 days. Results showed that the biofabrication methods used here succeeded in generating
a tubular structure consisting of a stromal substitute with a stratified epithelial-like layer on top,
especially using a medium containing epithelial growth and differentiation factors (EM), although
differentiation was not complete. At the functional level, UM substitutes were able to synthesize
collagen fibers, proteoglycans and glycosaminoglycans, although the levels of control UM were not
reached ex vivo. Epithelial differentiation was partially achieved, especially with EM after 14 days
of development, with expression of keratins 7, 8, and 13 and pancytokeratin, desmoplakin, tightjunction protein-1, and uroplakin 2, although at lower levels than controls. These results confirm
the partial urothelial differentiative potential of HWJSC and suggest that the biofabrication methods
explored here were able to generate a potential substitute of the human UM for future clinical use.CTS-115 Tissue Engineering Group and by the Spanish
Plan Nacional de Investigación Científica, Desarrollo e Innovación Tecnológica, Ministry of Science
and Innovation, Instituto de Salud Carlos III, grant FIS PI21/0981 (cofinanced by FEDER funds,
European Union)
Development of a diagnostic algorithm in periodontal disease and identification of genetic expression patterns: A preliminary report
AbstractBackground/purposeTo identify genetic expression patterns that can be used to define an appropriate diagnostic algorithm of clinical use in periodontal disease.Materials and methodsTotal RNA was extracted from 13 samples corresponding to normal human gingiva (NHG) and human gingiva affected by periodontal disease (PDHG). A comprehensive gene expression analysis was carried out by microarray analysis using Affymetrix Human Genome U133 plus 2.0 oligonucleotide arrays.ResultsSixty-six probe sets (genes and expressed sequence tags – EST) overexpressed in all samples of one of the comparison groups, were used for the diagnostic algorithm. All samples, including an independent test sample, were correctly classified as normal or periodontally affected using the diagnostic algorithm. In addition, 2596 genes/EST were upregulated and 1542 genes/EST were downregulated in PDHG, with numerous gene functions impaired in PDHG, especially those related to the immune response, cell-cell junctions, and extracellular matrix remodeling.ConclusionOur study reveals differential gene expression profiles in NHG and PDHG. The proposed diagnostic algorithm could have clinical usefulness for differential diagnosis in periodontal disease
Generation of genipin cross-linked fibrin-agarose hydrogel tissue-like models for tissue engineering applications
Generation of biomimetic and biocompatible artificial tissues is the basic research objective for tissue engineering (TE). In this sense, the biofabrication of scaffolds that resemble the tissues’ extracellular matrix (ECM) is an essential aim in this field. Uncompressed and nanostructured fibrin-agarose hydrogels (FAH and NFAH respectively) emerged as promising scaffold in TE, but its structure and biomechanical properties must be improved in order to broad their TE applications. Here we generated and characterized novel membranelike models with increased structural and biomechanical properties based on the chemical cross-linking of FAH and NFAH with genipin (GP at 0.1, 0.25, 0.5 and 0.75%). Furthermore, scaffolds were subjected to rheological (G, G’, G” modulus), ultrastructural and ex vivo biocompatibility analyses. Results showed that all GP concentrations increased the stiffness (G) and especially the elasticity (G’) of FAH and NFAH. Ultrastructural analyses demonstrated that GP and nanostructuration of FAH allowed controlling the porosity of FAH. In addition, biological studies revealed that higher concentration of GP significantly decreased the cell viability. Finally, this study demonstrated the possibility to generate natural FAH and NFAH with improved structural and biomechanical properties by using GP. However, further in vivo studies are needed in order to demonstrate the biocompatibility, biodegradability and regeneration capability of these cross-linked scaffolds
Histological and histochemical evaluation of human oral mucosa constructs developed by tissue engineering
Reconstruction of large oral mucosa defects
is often challenging, since the shortage of healthy oral mucosa to replace the excised tissues is very common. In this context, tissue engineering techniques may provide a
source of autologous tissues available for transplant in these patients. In this work, we developed a new model of artificial oral mucosa generated by tissue engineering
using a fibrin-agarose scaffold. For that purpose, we generated primary cultures of human oral mucosa fibroblasts and keratinocytes from small biopsies of
normal oral mucosa using enzymatic treatments. Then we determined the viability of the cultured cells by electron probe quantitative X-ray microanalysis, and we
demonstrated that most of the cells in the primary cultures were alive and had high K/Na ratios. Once cell viability was determined, we used the cultured fibroblasts and keratinocytes to develop an artificial oral mucosa construct by using a fibrin-agarose extracellular matrix and a sequential culture technique using porous
culture inserts. Histological analysis of the artificial tissues showed high similarities with normal oral
mucosa controls. The epithelium of the oral substitutes had several layers, with desmosomes and apical microvilli and microplicae. Both the controls and the
oral mucosa substitutes showed high suprabasal expression of cytokeratin 13 and low expression of cytokeratin 10. All these results suggest that our model of oral mucosa using fibrin-agarose scaffolds show
several similarities with native human oral mucosa.This work was supported by the grants FIS
03/0141 and FIS 04/1306 from the Spanish National Ministry of Health (Instituto de Salud Carlos III) and by CM 2005/011 from Junta de Andalucía
In Vitro Generation of Novel Functionalized Biomaterials for Use in Oral and Dental Regenerative Medicine Applications. Running Title: Fibrin–Agarose Functionalized Scaffolds
Supplementary Materials
The following are available online at https://www.mdpi.com/1996-1944/13/7/1692/s1, Table S1: Statistical comparison of staining intensity for histochemical and immunohistochemical analyses in the specific samples considered in the present study.Recent advances in tissue engineering offer innovative clinical alternatives in dentistry and regenerative medicine. Tissue engineering combines human cells with compatible biomaterials to induce tissue regeneration. Shortening the fabrication time of biomaterials used in tissue engineering will contribute to treatment improvement, and biomaterial functionalization can be exploited to enhance scaffold properties. In this work, we have tested an alternative biofabrication method by directly including human oral mucosa tissue explants within the biomaterial for the generation of human bioengineered mouth and dental tissues for use in tissue engineering. To achieve this, acellular fibrin–agarose scaffolds (AFAS), non-functionalized fibrin-agarose oral mucosa stroma substitutes (n-FAOM), and novel functionalized fibrin-agarose oral mucosa stroma substitutes (F-FAOM) were developed and analyzed after 1, 2, and 3 weeks of in vitro development to determine extracellular matrix components as compared to native oral mucosa controls by using histochemistry and immunohistochemistry. Results demonstrate that functionalization speeds up the biofabrication method and contributes to improve the biomimetic characteristics of the scaffold in terms of extracellular matrix components and reduce the time required for in vitro tissue development.Spanish Plan Nacional de Investigacion Cientifica, Desarrollo e Innovacion Tecnologica (I +D +I) of the Spanish Ministry of Economy and Competitiveness (Instituto de Salud Carlos III) (ERDF-FEDER, European Union)
FIS PI18/331
PI18/332Plan propio, Universidad de Granada
PSETC_19_001Consejeria de Economia, Conocimiento, Empresas y Universidad, Junta de Andalucia (European Regional Development Fund -FEDER
Applications of Tissue Engineering in reparation of abdominal wall defects
En este trabajo se ha realizado una revisión bibliográfica de más de treinta artículos relevantes que han sido
publicados en revistas científicas de impacto durante los últimos años con el objetivo de conocer la efectividad
de las nuevas opciones terapéuticas en la reparación de defectos de la pared abdominal. La Ingeniería Tisular
permite el desarrollo de nuevos sustitutos biológicos consistentes en matrices dérmicas acelulares sobre las
que se cultivan células de diverso origen. Estas nuevas mallas biológicas no presentan las complicaciones
habituales de las mallas artificiales que a día de hoy se utilizan en la clínica quirúrgica de forma rutinaria.
Además de reducir la incidencia de complicaciones como son la infección con necesidad de retirada de la
misma, falta de estímulo de la cicatrización o formación de seromas, estas nuevas opciones terapéuticas
aportan beneficios adicionales como favorecer la angiogénesis, biocompatibilidad, y por tanto disminución del
riesgo de infección. Existen varios tipos de matrices acelulares (humana, porcina, bovina,…) sin evidenciarse
diferencias significativas entre ellas, siendo la más utilizada la humana. El cultivo de células sobre estas
matrices ha demostrado mejores resultados que cuando se utiliza una matriz acelular. La colocación quirúrgica
de la malla parece influir en el porcentaje de recidivas, habiéndose mostrado la colocación pre-peritoneal y la
técnica de separación anatómica por componentes como las mejores. El principal problema que existe es que
los estudios tienen un período de seguimiento corto, por lo que aún no se conocen los resultados a largo plazo
de las mismas. A esto se añade el elevado coste de los biomateriales empleados, aún inviable en la práctica
quirúrgica diaria. Por todo ello es fundamental una mayor investigación de los nuevos biomateriales para poder
trasladar este tipo de opciones terapéuticas a la clínica, así como una adecuada selección del caso y el paciente
cuando se decida utilizarlas.In this work we have reviewed more than thirty relevant issues recently published in scientific journal with
impact factor in order to estimate the effectiveness of new therapeutic approaches in reparation of abdominal
wall defects. Tissue Engineering can be used for the development of new biological substitutes consisting
on acellular dermal matrix seeded with different types of cells. These new biological meshes do not present
some complications that are relatively common when artificial polymeric meshes are used in actual surgical
practice. Besides the decrease of incidences such as infection and need to removal, lack of healing signals, or
seromas, these new therapeutic approaches lead to some additional benefits such as to induce angiogenesis,
biocompatibility and, thus, decrease of infection risk. There are several types of acellular dermal matrix
(human, porcine, bovine …) with no significant evidence among them, although the most used is humanderived
matrix. Cell culture over these biomaterials has reported better results than using acellular matrix.
Matrix localization can also modify the relapse rate. In this sense, pre-peritoneal localization and anatomical
separation of components technique have been reported as the best techniques. The main concern about
the included studies is a short-term following period after treatment, and thus, scarce data about long-term
effectiveness of these therapeutic approaches. Furthermore, due to the high price of the used biomaterials,
these therapies are unfeasible nowadays in daily surgical practice. In summary, it is necessary advanced
research about these new biomaterials in order to translate them to daily practice, as well as an adequate
diagnosis and selection of the patient to be treated
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