EVALUATION OF IN VITRO INHIBITION OF PROTON􀇧PUMP BY NANOSILVER PARTICLES SYNTHESIZED USING SEEDS OF ANETHUM GRAVEOLENS

Objective: The green synthesis of nanoparticles is gaining popularity owing to its several advantages over the chemical methods. This study reports the biosynthesis of nanosilver mediated by the extract of the seeds of Anethum graveolens.Methods: Green synthesis of silver nanoparticles (AgNPs) was carried out using the aqueous extract of the seeds of A. graveolens (AESE). Microwave irradiation was done to hasten the process. The AgNPs synthesized were physically characterized using ultraviolet-visible spectroscopy, X-ray diffraction, Fourier transform infrared spectroscopy and scanning electron microscopy. The potential of the nanosilver particles in inhibiting H+ K+ adenosine triphosphatase (proton pump) was evaluated.Results: The nanosilver particles were initially subjected to characterization studies to ascertain their shape, size and surface composition. The particles were found to be crystalline, spherical of size 30-35 nm. The nanosilver particles inhibited the activity of proton pump by 67 % and thus were as potential as the standard drug, omeprazole.Conclusion: The seeds have several pharmacological benefits including its gastroprotective potential and hence the nanosilver particles synthesized using AESE was assessed for its gastroprotective potential. The results of this study prove that the nanosilver synthesized by green technology can inhibit proton-pump and thus act as promising gastroprotective agents

End to End Encrypted Smart Lock Using RSA based on Opinion from Social Media Review Comments

In the modern era, from big apartments to small houses, startups to corporate buildings, protecting assets or preventing unauthorized persons are crucial problems. Often traditional locks like padlocks are prone to security risks since they can be easily bypassed. Existing smart lock systems are prone to Man in middle Attacks where digital keys can easily be duplicated. The review comments about prevailing smart locks technologies have been collected from various sources such as blogs and microblogs.  The data set is analyzed to discover the opinion of the people about the smartlock product. In this proposed system, an innovative smartlock system prototype is designed using current technologies. A smart lock system has been proposed which is encrypted end-to-end using the RSA algorithm. This system uses a one-time password sent to registered users combined with the master code to unlock the door. This system is designed as such only the users who are connected to a wireless local area network are able to access the smart lock system, this adds an additional layer of security. It is connected to the cloud and logs all the activity from booting to shutting down. The breach detection system along with image capture is also included to detect forced intrusions. The client functionality can be easily ported to any platform which supports HTTP protocol which tends to be the major advantage of the proposed work

In vivo efficiency of Bacillus sp. isolated from biofloc system on growth, haematological, immunological and antioxidant status of genetically improved farmed tilapia (GIFT)

In aquaculture, Biofloc technology is emerging as one of the successful sustainable technologies to increase production. It is known to possess several immunostimulatory compounds exhibiting possible probiotic effect in culture. Here, we explored the in vivo efficiency of Bacillus infantis (T1), B. subtilis (T2), Exiguobacterium profundum (T3) and B. megaterium (T4) isolated from biofloc systems for improving the growth and immune performance of genetically improved farmed tilapia (GIFT). Animals (10+0.08 g) were stocked at a density of 100 per m-3 in 500 L FRP tanks for 42 days in triplicate. All the four probiotics (OD =1) were mixed with basal diet in treatments and feed without probiotic maintained as control. A significant difference (P <0.05) in weight gain, specific growth rate and FCR were observed between treatments and control with 100% survival. Serum albumin, globulin, protein, total blood count, glucose, myeloperoxidase activity and SOD were significantly different (P <0.05) between treatments and control. T4 and T2 showed better immunological and anti-oxidant ability when compared to other strains. Results from principal component analysis demonstrated that B. megaterium and B. subtilis can be the promising probiotic bacteria isolated from biofloc systems exhibiting multiple benefits with improved growth and health of the culture animals

In vivo efficiency of Bacillus sp. isolated from biofloc system on growth, haematological, immunological and antioxidant status of genetically improved farmed tilapia (GIFT)

714-721In aquaculture, Biofloc technology is emerging as one of the successful sustainable technologies to increase production. It is known to possess several immunostimulatory compounds exhibiting possible probiotic effect in culture. Here, we explored the in vivo efficiency of Bacillus infantis (T1), B. subtilis (T2), Exiguobacterium profundum (T3) and B. megaterium (T4) isolated from biofloc systems for improving the growth and immune performance of genetically improved farmed tilapia (GIFT). Animals (10+0.08 g) were stocked at a density of 100 per m-3 in 500 L FRP tanks for 42 days in triplicate. All the four probiotics (OD =1) were mixed with basal diet in treatments and feed without probiotic maintained as control. A significant difference (P 0.05) in weight gain, specific growth rate and FCR were observed between treatments and control with 100% survival. Serum albumin, globulin, protein, total blood count, glucose, myeloperoxidase activity and SOD were significantly different (P B. megaterium and B. subtilis can be the promising probiotic bacteria isolated from biofloc systems exhibiting multiple benefits with improved growth and health of the culture animals

Quantum spider prioritized routing and Cramer Shoup cryptosecured data transmission in wireless body area network

The wireless body area network (WBAN) offers healthcare applications for remote patient monitoring. In healthcare, prioritizing patient data has a high impact on the energy usage, delays, and congestion involved in routing. Energy usage, battery life, and absorption rates are current research obstacles in WBAN. The path loss ratio and residual energy were generally the focus of attention during data transfer. Despite this, compromised transaction security caused serious problems with the delivery of vital health data. The Quantum Spider Cramer Shoup Public Key Cryptosystem (QS-CSPKC), which is proposed in this study, is an optimized priority-aware (for many patients) and transaction security technique for vital sensed data in WBAN, selects the optimum energy efficient and reliable path to assure secure data transfer. The proposed QS-CSPKC method employs Dual Contention Access Periods (DCAPs) for managing regular data (i.e., normal sensed or low priority data) and life-threatening data (i.e., abnormal sensed data or high priority data). After prioritizing, the Quantum Spider Monkey Optimized Routing algorithm is used to allocate an optimal shortest route for normal low priority data (i.e., with 0 as the quantum value) and an energy-efficient emergency route for critical high priority data (i.e., with 1 as the quantum value). In addition, to prevent illegal access during transmission, security keys are generated using the Cramer Shoup public key cryptosystem. By using this model, only legitimate users have access to information for which they are authorized. The evaluation results were validated through simulations, showing that the proposed QS-CSPKC method performs efficient routing and secure data transmission better than conventional techniques in terms of throughput, packet loss, average power consumption, and energy use

Effect of in-situ and ex-situ biofloc on immune response of Genetically Improved Farmed Tilapia

Not AvailableThe present study is aimed to investigate the effect of biofloc intake on Genetically Improved Farmed Tilapia (GIFT), developed within the system and its influence as feed supplementation on water quality, growth performance, immunological parameters, antioxidant status, immune gene expression, and its resistance to Aeromonas hydrophila infection. GIFT Tilapia juveniles of 5.1 g (±0.05) were stocked at a density of 15/m3 in lined ponds of 300m2 in triplicates for 180 days. The experimental groups consisted of T1-biofloc developed within the culture systems (insitu), T2-biofloc supplementation in fish feed (exsitu) and C- Control without biofloc. Distillery Spent wash was used as a carbon source to maintain the C/N ratio of 10:1 for floc development in T1. Free CO2, pH, BOD, dissolved oxygen, alkalinity, Calcium and Magnesium ions, Nitrate, Nitrite and ammonia were found to be significantly different between the treatments and control throughout the experiment. The immunological (Serum protein, Respiratory burst test (RBT) and Myeloperoxidase) and antioxidant indicators (Glucose, Superoxide dismutase (SOD) and catalase) were found to be significantly higher in T1 at the end of the trial. Increased weight gain, specific growth rate, survival and decreased feed conversion ratio was found in T1 when compared with the other experimental groups. Real time quantitative PCR analysis revealed that there was no folded expression of the immunological genes such as Metallothionein gene, cathepsin L, Toll like receptor 7, Interleukin 1 β and Tumour necrosis factor α in liver and intestine for both control and treatment. However, the upregulated expression of targeted genes except tumour necrosis factor α was found in head kidney of T1. At the end of the study, GIFT Tilapia when infected with Aeromonas hydrophila showed an improved immune response in T1 and T2 with lesser signs of infection than Control. The findings of the present study affirmed the importance of biofloc technology in triggering the immunomodulatory response of GIFT Tilapia with its upregulated immune gene expression and its role as an antimicrobial agent against Aeromonas hydrophila. This study suggests the adoption of in-situ (T1) based biofloc method to obtain better performance of GIFT Tilapia culture.Not Availabl