Thermostable pectinase mediated enhanced antimicrobial activity of Emblica Officinalis: A novel application

1404-1410Four efficient pectinase producing thermophiles were isolated from Unhala hot water spring (16°35' N, 73°30' E) of Rajapur located in Ratnagiri district of Maharashtra. The isolates were identified as Bacillus licheniformis, Bacillus cereus, Bacillus Firmus, and Bacillus brevis using morphological and biochemical techniques. Being an efficient pectinase producer, Bacillus licheniformis was selected for pectinase production and further identified using 16s rRNA molecular analysis and sequence was deposited to GenBank nucleotide repository under accession number KT373817. Pectinase produced by Bacillus licheniformis using optimized condition was partially purified and molecular weight was determined as 66kda. Characterization of partially purified pectinase was carried out where it showed remarkable pectinolytic activity at 80oC temperature and pH 10.0. The extracted pectinase showed stability in presence of solvents and surfactants such as chloroform, ethanol, butanol, acetone, Tween-80 and Triton X-100 at 1% (v/v) concentration. Enhanced pectinolytic activity was observed in presence of Fe3+ ions (10 mM). Increase in antimicrobial activity of amla (Emblica Officinalis) was recorded after treatment with partially purified pectinase as a novel application for the first time

Permission based Mobile Malware Detection System using Machine Learning Techniques

Mobile technology has grown dramatically around the world. Nowadays smart mobile devices are ubiquitous, i.e. they serve multiple purposes such as personal mobile communication, data storage, multimedia and entertainment etc. They have become important part of life. Implementing secure mobile and wireless networks is crucial for enterprises operating in the Internet-based business environment. Mobile market share has grown significantly in past few years so that we need to think about mobile security. Mobile security can be compromised due to design flaws, vulnerabilities, and protocol failures in any mobile applications, viruses, spyware, malware and other threats. In this paper we will more focus on mobile malware. Many tools are available in the market to detect malware but new research trend in the mobile security is users should be aware of app before he/she installs from the app store. Hence we propose a novel approach for permission based mobile malware detection system. It is based on static analysis. It has 3 major parts in it 1) a signature database for storing analysis results of training and testing. 2) An Android client who is used by end users for making analysis requests, and 3) a central server plays important role as it communicates with both signature database and smartphone client. We can say that he is the manager of whole analysis process. It alerts user if the app is malicious or the benign based on it user can proceed whether to continue with it or not

Phytochemical analysis and antioxidant activities of Artemisia stelleriana Besser leaf extracts

The present study aims to report the proximate and mineral composition, phenolic contents, and antioxidant potential of Artemisia stelleriana leaves. The leaf extracts were prepared using various solvents like distilled water, methanol, ethanol, and acetone and analyzed for their phenolic and flavonoid contents and antioxidant activity. The methanolic extracts showed the highest total phenolic and flavonoid contents (10.09 ± 0.24 mg GAE/g and 225.04 ± 0.38 mg QE/ g, respectively). The methanolic extracts showed significantly higher 1,1-Diphenyl-2-picrylhydrazyl radical scavenging assay (DPPH-RSA), Reducing power assay, and total antioxidant capacity compared to distilled water, ethanol, and acetone extracts. Gas Chromatography-Mass Spectroscopy revealed that the methanolic extracts of leaves to be a good source of bioactive compounds like 2,4-di-tert-butylphenol (2,4-DTBP), neophytadiene, octacosane, and eucalyptol

REVIEW: FAST DISSOLVING TABLET

Fast dissolving tablets is one of the most widely accepted dosage forms and also most popular dosage form, especially for pediatric patients because of incomplete development of the muscular and nervous system and a case of geriatric patients suffering from Parkinson's disorder or hand tremors. Some solid dosage forms like tablets and capsules are present days facing the problems like difficulty in swallowing (dysphagia), resulting in many incidences of non-compliance and making the therapy ineffective. Oral dosage form and oral route are the most preferred route of administration for various drugs have limitations like the first-pass metabolism. Fast dissolving tablets are one of them. FDT have benefits such as accurate dosing, easy portability and manufacturing, good physical and chemical stability and an ideal alternative for pediatric and geriatric patients. Some tablets are designed to dissolve fastly in saliva, within a few seconds, and are true fast-dissolving tablets. Others contain agents to enhance the rate of tablet disintegration in the oral cavity and are more appropriately termed fast-disintegrating tablets, as they may take up to a minute to completely disintegrate

SARAS 2: A Spectral Radiometer for probing Cosmic Dawn and the Epoch of Reionization through detection of the global 21 cm signal

The global 21 cm signal from Cosmic Dawn (CD) and the Epoch of Reionization (EoR), at redshifts $z \sim 6-30$, probes the nature of first sources of radiation as well as physics of the Inter-Galactic Medium (IGM). Given that the signal is predicted to be extremely weak, of wide fractional bandwidth, and lies in a frequency range that is dominated by Galactic and Extragalactic foregrounds as well as Radio Frequency Interference, detection of the signal is a daunting task. Critical to the experiment is the manner in which the sky signal is represented through the instrument. It is of utmost importance to design a system whose spectral bandpass and additive spurious can be well calibrated and any calibration residual does not mimic the signal. SARAS is an ongoing experiment that aims to detect the global 21 cm signal. Here we present the design philosophy of the SARAS 2 system and discuss its performance and limitations based on laboratory and field measurements. Laboratory tests with the antenna replaced with a variety of terminations, including a network model for the antenna impedance, show that the gain calibration and modeling of internal additives leave no residuals with Fourier amplitudes exceeding 2~mK, or residual Gaussians of 25 MHz width with amplitudes exceeding 2~mK. Thus, even accounting for reflection and radiation efficiency losses in the antenna, the SARAS~2 system is capable of detection of complex 21-cm profiles at the level predicted by currently favoured models for thermal baryon evolution.Comment: 44 pages, 17 figures; comments and suggestions are welcom

Intrauterine contraceptive device migration to the urinary bladder

Intravesical migration represent as uncommon complication of intrauterine device insertion. We present a case of an IUD that migrated to the urinary bladder and impacted in urinary bladder wall, causing significant urinary symptoms. A 44 years old woman presented with menorrhagia, lower abdominal pain and urinary symptoms since past 1 year. She reported an IUD insertion 10 years back. Imaging detected the presence of IUD in the urinary bladder wall with large fibroid in uterus. Under anaesthesia, total abdominal hysterectomy with bilateral salphingoophorectomy is performed and IUD was removed out of the bladder without any complications. In her follow-up evaluation after 2 week, she had no urinary symptoms. A regular follow-up of IUD for visible thread would help in earlier detection of misplaced IUD and prevent the complications such as intravesical migration

Pulsatile Flow Through a Curved Pipe – An Analytical Study

In this paper, the unsteady flow of Newtonian fluid through a curved pipe due to a pulsatile pressure gradient has been considered. The flow is three dimensional and the partial differential equations governing the flow are highly coupled and non-linear. Approximate analytical solutions of the governing partial differential equations have been obtained without neglecting any term containing the curvature ratio. Perturbation series in terms of curvature ratio has been used for obtaining the solutions. It is interesting to note that the solutions are valid for large as well as small values of Womersley number. The effect of different parameters such as, Reynolds number, Womersley number and curvature ratio on the flow through curved pipe is discussed in this paper. It is found that the axial velocity is qualitatively periodic in nature, as expected. The Reynolds number and curvature ratio are found to shift the axial velocity towards the outer boundary of the curved pipe

Protection against diethylnitrosoamine-induced hepatocarcinogenesis by an indigenous medicine comprised of Nigella sativa, Hemidesmus indicus and Smilax glabra: a preliminary study

BACKGROUND: A decoction comprised of Nigella sativa seeds, Hemidesmus indicus root and Smilax glabra rhizome is used to treat cancer patients in Sri Lanka. However, the anti-carcinogenic properties of this decoction have not been experimentally confirmed. The purpose of this study was to determine whether the above decoction could protect against chemically induce hepatocarcinogenesis. METHODS: The effects of this decoction on diethylnitrosamine (DEN) induced hepatocarcinogenesis were examined in male Wistar rats using the medium term bioassay system of Ito, based on a 2-step model of hepatocarcinogenesis. Rats were randomly divided into 6 groups of 10 each. Groups 1 to 4 were injected with DEN (200 mg/kg) to initiate carcinogenesis. Twenty-four hours later groups 1 and 2 were administered the decoction at 4 g/kg body weight/day (dose 1) and 6 g/kg body weight/day (dose 2), respectively. Group 3 and group 4 were given distilled water instead of the decoction and a suspension of garlic powder (20 g/kg body weight/day) in distilled water (positive control), respectively. Group 5 and 6 were injected with normal saline and twenty-four hours later group 5 was given distilled water (normal control) while group 6 was given decoction dose 2 (decoction control). Oral feeding continued for two weeks after which all rats were subjected to 2/3 partial hepatectomy to promote carcinogenesis. Oral feeding continued for eight more weeks. At the end of the 10th week, rats were sacrificed and samples of livers taken for immunohistochemical studies. Carcinogenic potential was scored by comparing the number, area and staining intensity of glutathione S-transferase placental form (GST-P) positive foci and the number of cells/cm(2 )of the positive foci in the livers of the six groups of rats. RESULTS: The number and area of DEN-mediated GST-P positive foci, number of cells/cm(2 )of foci and staining intensity of the foci were significantly (P > 0.001) reduced by the decoction and garlic in the order dose 2 = garlic >dose 1. CONCLUSION: Overall results indicate that the decoction comprised of N. sativa, S. glabra and H. indicus has the potential to protect rat liver against DEN induced hepatocarcinogenesi