Mean platelet volume-to-platelet count ratio after elective cardiac surgical procedures is superior in reflecting platelets metabolic hyperactivity compared to other routine morphological platelet indices: A preliminary report

Background: Excessive metabolic excitation of platelets after cardiac procedures may be related to some adverse events but assessment of their metabolic activity is not routine. The purpose of this study was to evaluate which of the basic platelet morphological parameters best reflects their metabolic status. Methods: The blood samples of 22cardiac surgical patients (mean age of 62.3 ± 10.3 years) were taken before surgery (BS), and 1, 24 and 48 hours after the operation. Correlations between morphological platelet parameters (platelet count [PLT], mean platelet volume [MPV], platelet distribution width [PDW] and MPV/PLT) and their metabolic activity (total concentration of malondialdehyde [MDA] and MDA/PLT) were estimated. Results: Significant decline in PLT after operation (from 223 ± 44 × 1012/L to 166 ± 57 × 1012/L) was accompanied by marked increase in MPV (from 8.4 ± 0.9 fL to 9.1 ± 1.2 fL) and no change of PDW. Consequently, MPV/PLT index increased significantly after procedures from (median with IQR) 0.038 (0.030–0.043) to 0.053 (0.043–0.078). Simultaneously, a significant increase in total platelet MDA content and MDA/PLT was noted reaching peak levels soon after operation. The strongest correlation was observed between MPV/PLT and MDA/PLT (r = 0.56; p < 0.001), although the others were also found to be significant (MDA/PLT vs. MPV; r = 0.35; MDA/PLT vs. PDW; r = 0.34). Conclusions: Among basic morphological parameters and indices, the MPV-to-PLT ratio reflects the best metabolic status of platelets in cardiac surgical patients

The 42nd Symposium Chromatographic Methods of Investigating Organic Compounds : Book of abstracts

The 42nd Symposium Chromatographic Methods of Investigating Organic Compounds : Book of abstracts. June 4-7, 2019, Szczyrk, Polan

Proteininteraktionen, Analyse der Expression und Funktionsanalyse der PHF5a-Protein

Vier Proteine: U2AF35, SRp40, mDomino und Ddx1 wurden durch Screening Yeast-two hybride-Bibliothek als Partner des PHF 5a-Protein gefunden. Proteininteraktionen wurden in-vitro und in-vivo bestätigt. Die für Bindungen verantwortlichen Domäne wurden als RS Domäne (reich an Arginin-Serin) identifiziert. PHF5a funktioniert als "Brücke" zwischen ATP-Helikasen mDomino und Ddxl und Splicing Proteinen U2AF35 und SRp40. Forschungen Experimente mit der Anwendung von Fusions-Proteine und der Methode Färbung mit Antikörper haben bewiesen, dass PHF5a ein Zellkernprotein ist und mit U2AF35 und SRp40 kolokalisiert. Es wurde zusätzlich das Profil von Expression der Proteinen PHF5a und U2AF35 während der Embryoentwicklung und der Postnatalzellkernentwicklung analisier

Elastography as a new diagnostic tool to detect breast cancer – evaluation of research and clinical applications

Mammography and ultrasonography are currently the most sensitive methods for detecting breast cancer,but elastography is a new diagnostic tool. Stiffness of invasive carcinomas were found to be 5-25 times largerthan that of normal adipose tissue. There are basically two types of elastography examination used in initialclinical research: conventional elastography with compression and shear wave elastography. Analysis of 20 studiesin the years 1997-2010 with 1484 malignant and 2822 benign breast lesions is presented in this paper. Theoverall sensitivity was 67-100% with specificity of 62-99% for elastography. This raises the hope of introducingthis method in the BI-RADS classification and modifying diagnostics in category 3-4

Impact of the COVID-19 Pandemic on Attitudes toward Vaccination: Representative Study of Polish Society

The study explored the association between individuals’ attitudes toward vaccination and their actual vaccination behavior. We also examined the impact of the coronavirus disease 2019 (COVID-19) pandemic and the ongoing vaccination debate on changing attitudes towards vaccination, specifically within different demographic groups. The survey was conducted among a representative sample of Poles (N = 805) using computer-assisted web interview (CAWI) technology. As demonstrated, those who identified themselves as strong vaccine supporters were statistically significantly more frequently to be vaccinated with COVID-19 booster doses, to follow a physician’s recommendation on any vaccine without hesitation, and to be strengthened in their confidence in vaccines during the COVID-19 pandemic (p p p = 0.013, respectively). The results of this study imply that, in order to improve vaccine acceptance, it is essential to strengthen public health communication and avoid communication errors conducted during the COVID-19 pandemic

Is the shear wave sonographic elastography correlated with pain after breast augmentation with silicone implants an indication of inflammatory activity? A preliminary report

Introduction: Formation of a capsule is a natural inflammatory response to a foreign body such as a breast implant.Breast capsular contracture is the most severe complication of implant surgery. Aim: To evaluate breast tissues and the periprosthetic reaction with sonoelastography. Material and methods: Nineteen patients aged 20-41 underwent breast augmentation with silicone-filled implants.Their 38 breasts were evaluated before surgery, and 7 and 14 days after surgery. Whole breast stiffness was measuredby applanation tonometry. Patients underwent shear wave elastography and Young’s moduli of breast tissues and theperiprosthetic capsule were estimated. During surgery patients underwent standard anaesthesia and were releasedhome 2 days later after removal of drainage. Each day, patients completed the pain visual analogue scale questionnaireseparately for left and right breasts. Results: Applanation tonometry did not correlate with any parameter. In shear wave elastography we observed statisticallysignificant changes in elasticity of all breast tissues with the highest values on day 7 after surgery and decreasingon day 14. The correlations between pain and capsule elasticity in lower quadrants measured were significantbetween days 4 and 10, whereas correlations of pain with applanation tonometry were insignificant. Glandular tissueelasticity in lower quadrants did not correlate with pain, whereas in upper quadrants there was a significant correlationon days 6-10. Fatty tissue, muscle and thoracic fascia elasticity did not correlate with breast pain. Breast implantvolume correlated with pain only shortly after surgery, but did not correlate with any sonoelastographic parameters. Conclusions: Breast pain correlates strongly with periprosthetic stiffness in elastography 4 to 10 days after breast augmentation,suggesting the possible role of an inflammatory reaction