"Hazardous zones" within "security perimeter": comparative study on the material of folkloric discourse

The article considers the concept of "house" as a core element of "security perimeter" (the term by A. Leroy-Gourhan) on the material of English, Russian and Scottish ballad

Analysis of the influence of parametric noise on the dynamics of two interacting populations

This paper considers the predator-prey population model, which combines both the stabilizing factors of the intraspecific competition of prey and predator (for resources other than the prey), and the predator saturation. The purpose of this study is a comparative parametric analysis of stochastic phenomena which occur under parametric noise of two different types. The stochastic sensitivity of the attractors is studied. Based on the stochastic sensitivity function technique, noise-induced phenomena are described. In the parametric bistable zone, transitions of two types are carried out: equilibrium → equilibrium and cycle → equilibrium. The values of critical intensities for the occurrence of transition phenomena between attractors are obtained. In the parametric monostable zone, such phenomena as cycle deformation and equilibrium shift are demonstrated. © 2019 Udmurt State University. All right reserved.Russian Science Foundation, RSF: 16–11–10098Funding. The research was supported by the Russian Science Foundation (project no. 16–11–10098)

Dynamic regimes of the stochastic “prey – predatory” model with competition and saturation

We consider “predator – prey” model taking into account the competition of prey, predator for different from the prey resources, and their interaction described by the second type Holling trophic function. An analysis of the attractors is carried out depending on the coefficient of competition of predators. In the deterministic case, this model demonstrates the complex behavior associated with the local (Andronov – Hopf and saddle-node) and global (birth of a cycle from a separatrix loop) bifurcations. An important feature of this model is the disappearance of a stable cycle due to a saddle-node bifurcation. As a result of the presence of competition in both populations, parametric zones of mono- and bistability are observed. In parametric zones of bistability the system has either coexisting two equilibria or a cycle and equilibrium. Here, we investigate the geometrical arrangement of attractors and separatrices, which is the boundary of basins of attraction. Such a study is an important component in understanding of stochastic phenomena. In this model, the combination of the nonlinearity and random perturbations leads to the appearance of new phenomena with no analogues in the deterministic case, such as noise-induced transitions through the separatrix, stochastic excitability, and generation of mixed-mode oscillations. For the parametric study of these phenomena, we use the stochastic sensitivity function technique and the confidence domain method. In the bistability zones, we study the deformations of the equilibrium or oscillation regimes under stochastic perturbation. The geometric criterion for the occurrence of such qualitative changes is the intersection of confidence domains and the separatrix of the deterministic model. In the zone of monostability, we evolve the phenomena of explosive change in the size of population as well as extinction of one or both populations with minor changes in external conditions. With the help of the confidence domains method, we solve the problem of estimating the proximity of a stochastic population to dangerous boundaries, upon reaching which the coexistence of populations is destroyed and their extinction is observed. c 2019 Ekaterina P. Abramova, Tatyana V. Ryazanova.Russian Science Foundation, RSF: 16-11-10098This work was supported by Russian Science Foundation (No. 16-11-10098)

Tsar and his people: power and society in Moscow state during the second half of the 16th century

The article raises the issue about the nature of the relationship between the supreme power and society of the early Russian state. The authors of the article show that this unwritten contract, which was well understood by both parties, who participated in it, functioned well in Russia during the 16th - 17th centuries, and its existence refutes convincingly the Herberstein's passage, who failed to understand the Russian political realities of the early Modern Ag

Assessment of the Possibilities of Transition to Sustainable Environmental Management Models in the Arctic Regions

The sustainable development agenda for regional development can be developed in depth and comprehensively if considered in a cluster manner. It is advisable to distinguish the traditional clusters of sustainable development: economic, environmental and social. This paper considers the Environmental cluster, which has taken in the development of several Targets from the SDGs and conducted a study close to the assessment of the state of the environment. The purpose of the study is the possibility of updating the Arctic Council studies for the regions of the Russian Arctic in connection with their authentic regional ecological pressures. Objectives: to determine the actual environmental loads on each of the nine Arctic regions and to develop approaches to the formation of a model of sustainable environmental management within the framework of the existing scientific developments of the Arctic Council working groups and the formation of a scientific agenda for the future. Research methods: analysis of cartographic material, environmental reports of local and federal authorities, non-financial reports of enterprises operating in the Arctic regions, messengers and activity of civil society institutions, the real state of natural environments (atmospheric air, soil, water resources and forests), perspective development plans of the selected regions. As a result, several of the most urgent areas were highlighted in which it is advisable to focus further efforts to improve the state of the environment in each of the regions. The work was carried out during 2021-2022

Application of Basophil Activation Test with Anthraxin for Laboratory ( <I>in vitro</I>) Diagnostics of Anthrax

Demonstrated is the possibility to use in vitro basophil activation test with anthraxin, with registration of the results by means of flow cytometry, for anthrax diagnostics. This approach seems promising as it provides quantitative assessment of sensitization of the organism and does not cause its additional allergization. The duration of the analysis is 1h. The test is suggested for application, as an express one, for early and retrospective laboratory diagnostics of anthrax, estimation of post-vaccinal immunity

Self-monitoring level in communication and communicative skills of students of USMU AND URFU: a comparative analysis

The article reveals the importance of a high level of communication skills of a medical student for his further work. The results of testing to determine the level of communication skills are reviewed and their comparative analysis is carried out.В статье раскрыта важность высокого уровня коммуникативных навыков студентов медицинского вуза для дальнейшей профессиональной деятельности. Рассмотрены результаты ряда тестов на определение уровня владения коммуникативными навыками, проведен сравнительный анализ коммуникативных навыков студентов медицинского вуза и студентов технических специальностей

Development and validation of CAPS-marker associated with the <i>Rf2</i> gene in sorghum (<i>Sorghum bicolor</i> (L.) Moench)

Background. The development of heterotic hybrids based on cytoplasmic male sterility (CMS) is the leading strategy in breeding sorghum (Sorghum bicolor (L.) Moench). The trait of pollen fertility restoration in forms with CMS A1 (milo), predominantly used in sorghum breeding, is determined by at least two dominant complementary genes Rf1 and Rf2, and also gene Rf5. The development of accessible molecular markers of sorghum Rf genes is highly relevant for hybrid breeding, since they can significantly accelerate the process of creating female sterile forms (A lines), sterility maintainers (B lines) and pollen fertility restorers (R lines).Material and methods. The studied material included 36 sorghum accessions from the VIR collection, which differed by the ability to restore pollen fertility in forms with A1-type CMS. The nucleotide polymorphism of 935 bp fragments of the PPR genes Sobic.002G057050, Sobic.002G054100, and Sobic.002G054200 located at the chromosome 2 was studied.Results. The fragments obtained with the use of a pair of 2459403fw and 2459403 primers were 935 bp long and included parts of three genes: Sobic.002G057050, Sobic.002G054100, Sobic.002G054200. For identifying the sequence variant Sobic.002G057050-1090 associated with the Rf2 gene, Tru9 I restrictase was chosen, which allows obtaining a 572 bp fragment unique for all the studied R lines. Such a marker was found in 10 sorghum lines from West China and Kyrgyzstan, which are widely used in breeding as fertility restorers. The fragment was found neither in three lines with sterile cytoplasm and their fertile analogues, nor in 7 accessions of kafir sorghum, which lacked functional alleles of Rf genes.Conclusions. It has been demonstrated that the marker can be used for selection and checking purity of R and B/A lines. It is also applicable for verifying hybridity of F1 seeds and analyzing hybrid populations from crosses of R lines 924-4, 928-1, 929-3, 931-1, 933-1/6, 1237-3, 1243-2, 1251, 1150-1, F10BC2 with A lines Nizkorosloe 81s, А-83 and А-10598. It may be suggested that the ability to restore pollen fertility in R lines, which lack the marker CAPS- 572, is determined by another Rf gene. The studied 935 bp fragment of Sobic.002G057050 harbours 22 SNP, therefore the development of CAPS-markers for their identification and differentiation can be promising

Antibiotic Dosing in Chronic Kidney Disease

Infectious process is an important cause of morbidity and mortality among patients with chronic kidney disease. Prescription of antibacterial drugs should take into account the pharmacokinetic parameters of the medicine and the individual characteristics of the patient. Adequate antibiotic dosing is crucial for positive treatment outcome and minimisation of side effects. The aim of the study was to analyse scientific literature on factors affecting the dosing of antibacterials in patients with chronic kidney disease. Since most antibacterial medicines are eliminated by the kidneys, a decrease in glomerular filtration rate or kidney function should be followed by the dose adjustment in order to prevent the medicine accumulation and reduce the risk of side effects. Antibiotic dosing in such patients should be accompanied by kidney function assessment and be adjusted to ensure effective and safe treatment, as well as prevention of bacterial resistance. The review provides data on the dosing of some antibiotic groups (beta-lactams, aminoglycosides, fluoroquinolones) at different creatinine clearance rates. Extrarenal excretion of medicines does not usually require the dose adjustment in patients with chronic kidney disease

Evaluation of the Analytical Capabilities of MALDI-TOF Mass Spectrometry in the Molecular Typing of <i>Bacillus anthracis</i>

The aim of the work was to compare the discriminating capacity of the canSNP13 genotyping and MALDI‑TOF mass spectrometry methods based on the results of the study of anthrax pathogen strains belonging to the two main genetic lines A and B.Materials and methods. 73 Bacillus anthracis strains from the collection of microorganisms of the Stavropol Research Anti-Plague Institute of the Rospotrebnadzor were investigated. Protein profiling was carried out using a Microflex mass spectrometer, data processing – in the environment of the statistical programming language “R”.Results and discussion. It has been experimentally confirmed that the proposed approach for differentiating proteotypes of B. anthracis strains with a discrimination index of 0.952 exceeds the one for the canSNP typing method and is comparable to the discrimination index for the MLVA31 method. The correlation of the results of strain clustering during typing by MALDI-TOF mass spectrometry and canSNP genotyping reaches 95 % as regards the division into the main genetic lines A and B. The studied strains of anthrax microbe, which mostly fall under phylogenetic groups of lineage A, represent more than a dozen protein profiles, which may be due to differences in the level of protein expression in strains of each canSNP genotype. MALDI-TOF mass spectrometry makes it possible to obtain the results comparable with genetic tests, has a better discriminating capacity compared to canSNP typing, and is easier to perform