In Vivo Imaging Sample Image - Day 7 - White Light - Raw

This file is a raw jpeg generated for Fig 9. It depicts imaging of a mouse in group 3 imaged under white light as described in the methods section

Conjugate Characterization - Linker Stability

This file contains all HPLC chromatograms, graphs and computations for determining antibody-drug linker stability of the fluorescent antibody drug conjugate as described in the paper (Fig 2)

High-Resolution Mapping of Carbene-Based Protein Footprints

Carbene chemistry has been used recently in structural mass spectrometry as a labeling method for mapping protein surfaces. The current study presents a method for quantitating label distribution at the amino acid level and explores the nature and basis for an earlier observation of labeling bias. With the use of a method based on liquid chromatography–tandem mass spectrometry (LC–MS/MS) applied to digests of holo-calmodulin, we developed a quantitation strategy to map site-specific incorporation of carbene, generated from photolysis of ionic label precursors 2-amino-4,4-azipentanoic acid and 4,4-azipentanoic acid. The approach provides reliable incorporation data for fragments generated by electron-transfer dissociation, whereas high-energy collisional dissociation leads to energy and sequence-dependent loss of the label as a neutral. However, both can produce data suitable for mapping residues in the interaction of holo-calmodulin with M13 peptide ligand. Site-specific labeling was monitored as a function of reagent, ionic strength, and temperature, demonstrating that electrostatic interactions at the protein surface can “steer” the distribution of label precursors to sites of surface charge and favor label insertion into residues in the vicinity of the surface charge. A further preference for insertion into carboxylates was observed, based on chemical reactivity. We suggest that decoupling surface partitioning from the chemistry of insertion offers a flexible, tunable labeling strategy for structural mass spectrometry that can be applied to a broad range of protein surface compositions and promotes the design of reagents to simplify the workflow

In Vitro Fluorescent Evaluation - CEA Internalization Data

This file contains the compiled and processed data surrounding the quantitative estimation of CEA internalization (Fig 5, panel B)

IC₅₀ Estimations of Samples and Conjugates.

<p>IC₅₀ Estimations of Samples and Conjugates.</p

In Vivo Evaluation - Tumor Growth and Auto Exposure Data

This excel file contains all raw tumor growth measurements and graphs for in vivo efficacy evaluation (Fig 8). This file also contains the compiled exposure times generated for Fig 10

In Vitro Fluorescent Evaluation - BxPC3 Flow Data

This file contains the raw flow cytometry data and overlayed images (“analyze” tab) which were arranged for Fig 4

In Vitro Fluorescent Evaluation - HeLa Flow Data

This file contains the raw flow cytometry data and overlayed images (“analyze” tab) which were arranged for Fig 4

Immunofluorescence Sample Image BxPC3 IgG 680

This file is a raw jpeg of an immunofluorescent image used in Fig 3, panel A

Immunofluorescence Sample Image BxPC-3 CEA 680 PTX

This file is a raw jpeg of an immunofluorescent image used in Fig 3, panel A