27 research outputs found
In Vivo Imaging Sample Image - Day 7 - White Light - Raw
This file is a raw jpeg generated for Fig 9. It depicts imaging of a mouse in group 3 imaged under white light as described in the methods section
Conjugate Characterization - Linker Stability
This file contains all HPLC chromatograms, graphs and computations for determining antibody-drug linker stability of the fluorescent antibody drug conjugate as described in the paper (Fig 2)
High-Resolution Mapping of Carbene-Based Protein Footprints
Carbene chemistry has been used recently in structural
mass spectrometry
as a labeling method for mapping protein surfaces. The current study
presents a method for quantitating label distribution at the amino
acid level and explores the nature and basis for an earlier observation
of labeling bias. With the use of a method based on liquid chromatography–tandem
mass spectrometry (LC–MS/MS) applied to digests of holo-calmodulin,
we developed a quantitation strategy to map site-specific incorporation
of carbene, generated from photolysis of ionic label precursors 2-amino-4,4-azipentanoic
acid and 4,4-azipentanoic acid. The approach provides reliable incorporation
data for fragments generated by electron-transfer dissociation, whereas
high-energy collisional dissociation leads to energy and sequence-dependent
loss of the label as a neutral. However, both can produce data suitable
for mapping residues in the interaction of holo-calmodulin
with M13 peptide ligand. Site-specific labeling was monitored as a
function of reagent, ionic strength, and temperature, demonstrating
that electrostatic interactions at the protein surface can “steer”
the distribution of label precursors to sites of surface charge and
favor label insertion into residues in the vicinity of the surface
charge. A further preference for insertion into carboxylates was observed,
based on chemical reactivity. We suggest that decoupling surface partitioning
from the chemistry of insertion offers a flexible, tunable labeling
strategy for structural mass spectrometry that can be applied to a
broad range of protein surface compositions and promotes the design
of reagents to simplify the workflow
In Vitro Fluorescent Evaluation - CEA Internalization Data
This file contains the compiled and processed data surrounding the quantitative estimation of CEA internalization (Fig 5, panel B)
IC<sub>50</sub> Estimations of Samples and Conjugates.
<p>IC<sub>50</sub> Estimations of Samples and Conjugates.</p
In Vivo Evaluation - Tumor Growth and Auto Exposure Data
This excel file contains all raw tumor growth measurements and graphs for in vivo efficacy evaluation (Fig 8). This file also contains the compiled exposure times generated for Fig 10
In Vitro Fluorescent Evaluation - BxPC3 Flow Data
This file contains the raw flow cytometry data and overlayed images (“analyze” tab) which were arranged for Fig 4
In Vitro Fluorescent Evaluation - HeLa Flow Data
This file contains the raw flow cytometry data and overlayed images (“analyze” tab) which were arranged for Fig 4
Immunofluorescence Sample Image BxPC3 IgG 680
This file is a raw jpeg of an immunofluorescent image used in Fig 3, panel A
Immunofluorescence Sample Image BxPC-3 CEA 680 PTX
This file is a raw jpeg of an immunofluorescent image used in Fig 3, panel A