CFA-treated Mice Induce Hyperalgesia in Healthy mice via an olfactory mechanism

Background Social interactions with subjects experiencing pain can increase nociceptive sensitivity in observers, even without direct physical contact. In previous experiments, extended indirect exposure to soiled bedding from mice with alcohol withdrawal-related hyperalgesia enhanced nociception in their conspecifics. This finding suggested that olfactory cues could be sufficient for nociceptive hypersensitivity in otherwise untreated animals (also known as “bystanders”). Aim The current study addressed this possibility using an inflammation-based hyperalgesia model and long- and short-term exposure paradigms in C57BL/6J mice. Materials & Method Adult male and female mice received intraplantar injection of complete Freund\u27s adjuvant (CFA) and were used as stimulus animals to otherwise naïve same-sex bystander mice (BS). Another group of untreated mice (OLF) was simultaneously exposed to the bedding of the stimulus mice. Results In the long-term, 15-day exposure paradigm, the presence of CFA mice or their bedding resulted in reduced von Frey threshold but not Hargreaves paw withdrawal latency in BS or OLF mice. In the short-term paradigm, 1-hr interaction with CFA conspecifics or 1-hr exposure to their bedding induced mechanical hypersensitivity in BS and OLF mice lasting for 3 hrs. Chemical ablation of the main olfactory epithelium prevented bedding-induced and stimulus mice-induced mechanical hypersensitivity. Gas chromatography-mass spectrometry (GC-MS) analysis of the volatile compounds in the bedding of experimental mice revealed that CFA-treated mice released an increased number of compounds indicative of disease states

Long-term operation of a multi-channel cosmic muon system based on scintillation counters with MRS APD light readout

A Cosmic Ray Test Facility (CRTF) is the first large-scale implementation of a scintillation triggering system based on a new scintillation technique known as START. In START, the scintillation light is collected and transported by WLS optical fibers, while light detection is performed by pairs of avalanche photodiodes with the Metal-Resistor-Semiconductor structure operated in the Geiger mode (MRS APD). START delivers 100% efficiency of cosmic muon detection, while its intrinsic noise level is less than 10^{-2} Hz. CRTF, consisting of 160 START channels, has been continuously operated by the ALICE TOF collaboration for more than 25 000 hours, and has demonstrated a high level of stability. Fewer than 10% of MRS APDs had to be replaced during this period.Comment: Proceedings of NDIP-2008. 8 pages, 8 figures, 6 reference

Scintillation counter with MRS APD light readout

START, a high-efficiency and low-noise scintillation detector for ionizing particles, was developed for the purpose of creating a high-granular system for triggering cosmic muons. Scintillation light in START is detected by MRS APDs (Avalanche Photo-Diodes with Metal-Resistance-Semiconductor structure), operated in the Geiger mode, which have 1 mm^2 sensitive areas. START is assembled from a 15 x 15 x 1 cm^3 scintillating plastic plate, two MRS APDs and two pieces of wavelength-shifting optical fiber stacked in circular coils inside the plastic. The front-end electronic card is mounted directly on the detector. Tests with START have confirmed its operational consistency, over 99% efficiency of MIP registration and good homogeneity. START demonstrates a low intrinsic noise of about 10^{-2} Hz. If these detectors are to be mass-produced, the cost of a mosaic array of STARTs is estimated at a moderate level of 2-3 kUSD/m^2.Comment: 6 pages, 5 figure

Control of chronic excessive alcohol drinking by genetic manipulation of the Edinger-Westphal nucleus urocortin-1 neuropeptide system

Midbrain neurons of the centrally projecting Edinger-Westphal nucleus (EWcp) are activated by alcohol, and enriched with stress-responsive neuropeptide modulators (including the paralog of corticotropin-releasing factor, urocortin-1). Evidence suggests that EWcp neurons promote behavioral processes for alcohol-seeking and consumption, but a definitive role for these cells remains elusive. Here we combined targeted viral manipulations and gene array profiling of EWcp neurons with mass behavioral phenotyping in C57BL/6 J mice to directly define the links between EWcp-specific urocortin-1 expression and voluntary binge alcohol intake, demonstrating a specific importance for EWcp urocortin-1 activity in escalation of alcohol intake

Arctic sea-ice change: a grand challenge of climate science

Over the period of modern satellite observations, Arctic sea-ice extent at the end of the melt season (September) has declined at a rate of >11% per decade, and there is evidence that the rate of decline has accelerated during the last decade.While climate models project further decreases in seaice mass and extent through the 21st century, the model ensemble mean trend over the period of instrumental records is smaller than observed. Possible reasons for the apparent discrepancy between observations and model simulations include observational uncertainties, vigorous unforced climate variability in the high latitudes, and limitations and shortcomings of the models stemming in particular from gaps in understanding physical process. The economic significance of a seasonally sea-ice-free future Arctic, the increased connectivity of a warmer Arctic with changes in global climate, and large uncertainties in magnitude and timing of these impacts make the problem of rapid sea-ice loss in the Arctic a grand challenge of climate science. Meaningful prediction/projection of the Arctic sea-ice conditions for the coming decades and beyond requires determining priorities for observations and model development, evaluation of the ability of climate models to reproduce the observed sea-ice behavior as a part of the broader climate system, improved attribution of the causes of Arctic sea-ice change, and improved understanding of the predictability of sea-ice conditions on seasonal through centennial timescales in the wider context of the polar climate predictability

START as the detector of choice for large-scale muon triggering systems

Further progress in building high-granular large-scale systems based on Scintillation Tiles with MRS APD light readout (START) became possible thanks to the creation of an improved version of MRS APD. The cost of the system may now be significantly reduced by using inexpensive extruded scintillator. More than 160 START samples were assembled based on this design modification and proved to possess 100% MIP detection efficiency and the intrinsic noise rate of less than 0.08 Hz. Long-term stability of START characteristics was confirmed after 3.5 months of operation

Urocortin-1 within the Centrally-Projecting Edinger-Westphal Nucleus Is Critical for Ethanol Preference

Converging lines of evidence point to the involvement of neurons of the centrally projecting Edinger-Westphal nucleus (EWcp) containing the neuropeptide Urocortin-1 (Ucn1) in excessive ethanol (EtOH) intake and EtOH sensitivity. Here, we expanded these previous findings by using a continuous-access, two-bottle choice drinking paradigm (3%, 6%, and 10% EtOH vs. tap water) to compare EtOH intake and EtOH preference in Ucn1 genetic knockout (KO) and wild-type (WT) mice. Based on previous studies demonstrating that electrolytic lesion of the EWcp attenuated EtOH intake and preference in high-drinking C57BL/6J mice, we also set out to determine whether EWcp lesion would differentially alter EtOH consumption in Ucn1 KO and WT mice. Finally, we implemented well-established place conditioning procedures in KO and WT mice to determine whether Ucn1 and the corticotropin-releasing factor type-2 receptor (CRF-R2) were involved in the rewarding and aversive effects of EtOH (2 g/kg, i.p.). Results from these studies revealed that (1) genetic deletion of Ucn1 dampened EtOH preference only in mice with an intact EWcp, but not in mice that received lesion of the EWcp, (2) lesion of the EWcp dampened EtOH intake in Ucn1 KO and WT mice, but dampened EtOH preference only in WT mice expressing Ucn1, and (3) genetic deletion of Ucn1 or CRF-R2 abolished the conditioned rewarding effects of EtOH, but deletion of Ucn1 had no effect on the conditioned aversive effects of EtOH. The current findings provide strong support for the hypothesis that EWcp-Ucn1 neurons play an important role in EtOH intake, preference, and reward

Cross-Correlation Earthquake Precursors in the Hydrogeochemical and Geoacoustic Signals for the Kamchatka Peninsula

We propose a new type of earthquake precursor based on the analysis of correlation dynamics between geophysical signals of different nature. The precursor is found using a two-parameter cross-correlation function introduced within the framework of flicker-noise spectroscopy, a general statistical physics approach to the analysis of time series. We consider an example of cross-correlation analysis for water salinity time series, an integral characteristic of the chemical composition of groundwater, and geoacoustic emissions recorded at the G-1 borehole on the Kamchatka peninsula in the time frame from 2001 to 2003, which is characterized by a sequence of three groups of significant seismic events. We found that cross-correlation precursors took place 27, 31, and 35 days ahead of the strongest earthquakes for each group of seismic events, respectively. At the same time, precursory anomalies in the signals themselves were observed only in the geoacoustic emissions for one group of earthquakes.Comment: 21 pages, 5 figures, 1 table; to be published in "Acta Geophysica". arXiv admin note: substantial text overlap with arXiv:1101.147

Universal Oligonucleotide Microarray for Sub-Typing of Influenza A Virus

A universal microchip was developed for genotyping Influenza A viruses. It contains two sets of oligonucleotide probes allowing viruses to be classified by the subtypes of hemagglutinin (H1–H13, H15, H16) and neuraminidase (N1–N9). Additional sets of probes are used to detect H1N1 swine influenza viruses. Selection of probes was done in two steps. Initially, amino acid sequences specific to each subtype were identified, and then the most specific and representative oligonucleotide probes were selected. Overall, between 19 and 24 probes were used to identify each subtype of hemagglutinin (HA) and neuraminidase (NA). Genotyping included preparation of fluorescently labeled PCR amplicons of influenza virus cDNA and their hybridization to microarrays of specific oligonucleotide probes. Out of 40 samples tested, 36 unambiguously identified HA and NA subtypes of Influenza A virus

The Role of Early Life Experience and Species Differences in Alcohol Intake in Microtine Rodents

Social relationships have important effects on alcohol drinking. There are conflicting reports, however, about whether early-life family structure plays an important role in moderating alcohol use in humans. We have previously modeled social facilitation of alcohol drinking in peers in socially monogamous prairie voles. We have also modeled the effects of family structure on the development of adult social and emotional behaviors. Here we assessed whether alcohol intake would differ in prairie voles reared by both parents compared to those reared by a single mother. We also assessed whether meadow voles, a closely related species that do not form lasting reproductive partnerships, would differ in alcohol drinking or in the effect of social influence on drinking. Prairie voles were reared either bi-parentally (BP) or by a single mother (SM). BP- and SM-reared adult prairie voles and BP-reared adult meadow voles were given limited access to a choice between alcohol (10%) and water over four days and assessed for drinking behavior in social and non-social drinking environments. While alcohol preference was not different between species, meadow voles drank significantly lower doses than prairie voles. Meadow voles also had significantly higher blood ethanol concentrations than prairie voles after receiving the same dose, suggesting differences in ethanol metabolism. Both species, regardless of rearing condition, consumed more alcohol in the social drinking condition than the non-social condition. Early life family structure did not significantly affect any measure. Greater drinking in the social condition indicates that alcohol intake is influenced similarly in both species by the presence of a peer. While the ability of prairie voles to model humans may be limited, the lack of differences in alcohol drinking in BP- and SM-reared prairie voles lends biological support to human studies demonstrating no effect of single-parenting on alcohol abuse