The proangiogenic capacity of polymorphonuclear neutrophils delineated by microarray technique and by measurement of neovascularization in wounded skin of CD18-deficient mice

Growing evidence supports the concept that polymorphonuclear neutrophils (PMN) are critically involved in inflammation-mediated angiogenesis which is important for wound healing and repair. We employed an oligonucleotide microarray technique to gain further insight into the molecular mechanisms underlying the proangiogenic potential of human PMN. In addition to 18 known angiogenesis-relevant genes, we detected the expression of 10 novel genes, namely midkine, erb-B2, ets-1, transforming growth factor receptor-beta(2) and -beta(3), thrombospondin, tissue inhibitor of metalloproteinase 2, ephrin A2, ephrin B2 and restin in human PMN freshly isolated from the circulation. Gene expression was confi rmed by the RT-PCR technique. In vivo evidence for the role of PMN in neovascularization was provided by studying neovascularization in a skin model of wound healing using CD18-deficient mice which lack PMN infi ltration to sites of lesion. In CD18-deficient animals, neo- vascularization was found to be signifi cantly compromised when compared with wild- type control animals which showed profound neovascularization within the granulation tissue during the wound healing process. Thus, PMN infiltration seems to facilitate inflammation mediated angiogenesis which may be a consequence of the broad spectrum of proangiogenic factors expressed by these cells. Copyright (c) 2006 S. Karger AG, Basel

The Changing Landscape for Stroke\ua0Prevention in AF: Findings From the GLORIA-AF Registry Phase 2

Background GLORIA-AF (Global Registry on Long-Term Oral Antithrombotic Treatment in Patients with Atrial Fibrillation) is a prospective, global registry program describing antithrombotic treatment patterns in patients with newly diagnosed nonvalvular atrial fibrillation at risk of stroke. Phase 2 began when dabigatran, the first non\u2013vitamin K antagonist oral anticoagulant (NOAC), became available. Objectives This study sought to describe phase 2 baseline data and compare these with the pre-NOAC era collected during phase 1. Methods During phase 2, 15,641 consenting patients were enrolled (November 2011 to December 2014); 15,092 were eligible. This pre-specified cross-sectional analysis describes eligible patients\u2019 baseline characteristics. Atrial fibrillation disease characteristics, medical outcomes, and concomitant diseases and medications were collected. Data were analyzed using descriptive statistics. Results Of the total patients, 45.5% were female; median age was 71 (interquartile range: 64, 78) years. Patients were from Europe (47.1%), North America (22.5%), Asia (20.3%), Latin America (6.0%), and the Middle East/Africa (4.0%). Most had high stroke risk (CHA2DS2-VASc [Congestive heart failure, Hypertension, Age  6575 years, Diabetes mellitus, previous Stroke, Vascular disease, Age 65 to 74 years, Sex category] score  652; 86.1%); 13.9% had moderate risk (CHA2DS2-VASc = 1). Overall, 79.9% received oral anticoagulants, of whom 47.6% received NOAC and 32.3% vitamin K antagonists (VKA); 12.1% received antiplatelet agents; 7.8% received no antithrombotic treatment. For comparison, the proportion of phase 1 patients (of N = 1,063 all eligible) prescribed VKA was 32.8%, acetylsalicylic acid 41.7%, and no therapy 20.2%. In Europe in phase 2, treatment with NOAC was more common than VKA (52.3% and 37.8%, respectively); 6.0% of patients received antiplatelet treatment; and 3.8% received no antithrombotic treatment. In North America, 52.1%, 26.2%, and 14.0% of patients received NOAC, VKA, and antiplatelet drugs, respectively; 7.5% received no antithrombotic treatment. NOAC use was less common in Asia (27.7%), where 27.5% of patients received VKA, 25.0% antiplatelet drugs, and 19.8% no antithrombotic treatment. Conclusions The baseline data from GLORIA-AF phase 2 demonstrate that in newly diagnosed nonvalvular atrial fibrillation patients, NOAC have been highly adopted into practice, becoming more frequently prescribed than VKA in Europe and North America. Worldwide, however, a large proportion of patients remain undertreated, particularly in Asia and North America. (Global Registry on Long-Term Oral Antithrombotic Treatment in Patients With Atrial Fibrillation [GLORIA-AF]; NCT01468701

Identifying important marine areas for pinnipeds : a comparison of approaches

Verkefnið er lokað til 17.6.2018.Given the widespread decline of species and threats to habitats across the globe, identifying important areas for conservation is now more important than ever. With ever-improving technology, the use of tracking data has recently contributed valuable information on the movement of marine organisms, and has allowed for insight into habits and habitat use of animals such as pinnipeds that are difficult to study. For example, Important Bird and Biodiversity Areas (IBAs) have been identified using seabird tracking data (BirdLife International (BI) approach). In this study the applicability of the BI approach to pinniped data was tested, based on similarities in foraging patterns and attendance cycles between seabirds and female pinnipeds during their breeding season. PTT and GPS data from nine species were analysed using the BI approach and compared to three commonly used methods of spatial analysis; Kernel Density Estimation, Minimum Convex Polygon, and Time-spent-in-Area analyses. The 50% home range was used to analyse the size of areas and the percentage overlap between areas identified by different methods. The BI approach and other methods were all able to effectively identify important areas for ten of eleven data groups, though the areas identified by all four methods differed in their extent and exact location. Advantages and disadvantages of each method should be taken into account in conservation management though this study indicates that the BI approach is a good compromise between these. Future developments could include broadening of the approach to incorporate a wider range of pinniped species

Property, Progeny, and Emotion: Family History in a Leonese Village

This article considers, from the historical, demographic, and anthropological points of view the various forms that the Leonese peasant family takes during its development cycle, and demonstrates the importance of extended and multiple family households in an area long characterized by partible inheritance and nuclear family households. Two methods of doing family history, at times held incompatible, are used and are shown to be complementary: a structural analysis of households, based on four household lists from 1920 to 1978, and on demographic data from 1739 to 1978; and an interpretive analysis of the lived reality of the Leonese household, based on ethnographic data and on locally held notions of proper relations between kin, as embedded in stories people tell about family histories.Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/67451/2/10.1177_036319908801300102.pd

The ABC‐Stroke Risk Score and Effects of Atrial Fibrillation Screening on Stroke Prevention:Results From the Randomized LOOP Study

BACKGROUND: The ABC-stroke score is a risk scheme for prediction of stroke or systemic embolism (SE) in atrial fibrillation (AF). This study sought to examine whether the score could be useful in predicting stroke in AF-naïve individuals and risk stratifying for AF screening. METHODS AND RESULTS: The LOOP (Atrial Fibrillation Detected by Continuous ECG Monitoring Using Implantable Loop Recorder to Prevent Stroke in High-Risk Individuals) study randomized 6004 AF-naïve individuals aged 70 to 90 years with stroke risk factors to either screening with an implantable loop recorder and anticoagulation upon detection of new-onset AF episodes ≥6 minutes, or usual care. A total of 5781 participants had available ABC-stroke score at baseline and were included in this secondary analysis: 4170 (72.1%) with an estimated stroke/SE risk ≤1%/year versus 1611 (27.9%) with an estimated stroke/SE risk &gt;1%/year. Having an annual ABC-stroke risk &gt;1% was associated with stroke/SE, stroke/SE/cardiovascular death, and allcause death (hazard ratio, 1.82 [95% CI, 1.44–2.21], 2.17 [95% CI, 1.80–2.62], and 2.19 [95% CI, 1.87–2.56], respectively). For screening with implantable loop recorder versus usual care, no significant reduction in these study outcomes was obtained in any ABC-stroke risk groups (P&gt;0.0500 for all), with no signal toward interaction (P interaction &gt;0.2500 for all). Similar findings were yielded when assessing the ABC-stroke score as a continuous variable. CONCLUSIONS: In an elderly, AF-naïve population with additional stroke risk factors, a higher ABC-stroke score could identify individuals with increased stroke risk. However, this risk score may not be useful in pinpointing those more likely to benefit from AF screening and subsequent preventive treatment. These findings should be considered as hypothesis generating and warrant further study.</p

A Hard Day at Work: An Analysis of Occupational Genitourinary Injuries in the United States Workforce.

OBJECTIVE: To use national data to identify risk factors for occupational genitourinary (GU) injuries and to expose potential workplace safety issues requiring national regulation. METHODS: The National Trauma Data Bank was queried to identify all adults who suffered a work-related GU injury from 2007-2016. Injury was stratified by individual organ and by organ type: intra-abdominopelvic (IAP) versus external genitalia (EG). Distinct multivariable logistic regression models were used to examine associations between prespecified risk factors and GU injury (organ and type) and to identify predictors of intensive care unit (ICU) and operating room (OR) transfer. RESULTS: 2139 patients (total of 2681 GU injuries), were included. A mean of 1.3 GU organ injuries and 7.6 total injuries were suffered per patient. 72% suffered an IAP GU injury, 23% an EG injury, and 5% suffered both. Patients working in agriculture/forestry/fishing, (OR 2.3, p=0.003), manufacturing (OR 1.9, p=0.05), and natural resources/mining (OR 2.3, p= 0.012) were at significantly increased risk of EG injury. The penis and urethra were particularly at-risk in agriculture/forestry/fishing (OR 4.0, p=0.005; OR 3.0, p=0.002) and the urethra in natural resources/mining (OR 3.4, p=0.004). IAP GU injury was a significant predictor of ICU transfer (OR 1.8, p \u3c 0.001), whereas EG injury was a significant predictor of OR transfer (OR 2.5, p \u3c 0.001). CONCLUSIONS: Occupational GU injuries remain a major issue for blue-collar workers. External genitalia are particularly at-risk, and injuries often require emergent surgery. National occupational health agencies need to continue to enhance on-the-job safety for those at-risk