4 research outputs found

    SCALE UP OF CYCLONE BIOREACTOR FOR THE PRODUCTION OF BIODEGRADABLE PLASTICS

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    The production of microbial poly-ß-hydroxybutyrate (PHB) was studied usingcyclone bioreactors at both the 1 L lab-scale and in 75 L Pilot Plant Scale (PPS) ofPilot Plant scale. The Culture used for PHB production was Alcaligenes eutrophusDSM 545, grown on a mineral salt medium limited by the supply of nitrogen. Twodifferent carbon sources were used: standard commercial glucose and hydrolyzedpotato processing waste. The levels of dissolved oxygen obtained in the PPS werestrongly depended on the location at which the air was introduced into thereactor. However, with aeration balanced between two injection points and asimilar level of power input, 17 W/L, the PPS was able to provide at least as muchoxygen transfer capability as the lab-scale reactor. Under all condition tested, thePHB accumulation by A. eutrophus was very high, in excess of 80% of thebiomass dry weight. The potato starch was readily hydrolyzed to fermentablesugars, however, the economic feasibility of using this carbon sources requiresfurther evaluation

    Scale Up of Cyclone Bioreactor for the Production of Biodegradable Plastics

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    The production of microbial poly-ß-hydroxybutyrate (PHB) was studied usingcyclone bioreactors at both the 1 L lab-scale and in 75 L Pilot Plant Scale (PPS) ofPilot Plant scale. The Culture used for PHB production was Alcaligenes eutrophusDSM 545, grown on a mineral salt medium limited by the supply of nitrogen. Twodifferent carbon sources were used: standard commercial glucose and hydrolyzedpotato processing waste. The levels of dissolved oxygen obtained in the PPS werestrongly depended on the location at which the air was introduced into thereactor. However, with aeration balanced between two injection points and asimilar level of power input, 17 W/L, the PPS was able to provide at least as muchoxygen transfer capability as the lab-scale reactor. Under all condition tested, thePHB accumulation by A. eutrophus was very high, in excess of 80% of thebiomass dry weight. The potato starch was readily hydrolyzed to fermentablesugars, however, the economic feasibility of using this carbon sources requiresfurther evaluation

    Enzymatic hydrolysis of potato processing waste for the production of biopolymers

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    Biopolymers are polymers produced by certain microorganisms, that are readily degradable in the environment. These biodegradable plastics have the potential to be used as substitutes for conventional petroleum based plastic provided that the production costs can be greatly reduced. The high cost of biopolymer production is due to the cost of substrate which mainly is glucose.The enzymatic hydrolysis of potato processing wastes was to produce glucose as a least expensive feedstock substrate for the production of biopolymers of polyhydroxybutirate (PHB) from the bacterium Alcaligenes eutrophus was studied. The enzymatic hydrolysis experiments were carried out using alpha alpha-amylase liquefaction enzymes from Aspergillus oryzae and barley-malt, and amyloglucosidase saccharification enzyme from Rhizopus.The results indicated that the production of glucose from potato starch waste to be used as a substrate to produce biopolymers was both technically and economically feasible. A 10 to 90 ratio of barley-malt to potato starch waste gave the highest conversion of starch to glucose of 194.30 gLsp1 sp{-1} (96.56%), and the lowest liquefaction enzyme cost (0.054)tohydrolyzeonekgofpotatostarchwaste.itA.eutrophus/producedPHBof5.0gL0.054) to hydrolyze one kg of potato starch waste. { it A. eutrophus /} produced PHB of 5.0 gL sp-1$ (76.9 % of biomass) using the glucose substrate generated from the potato starch waste

    BIOKONVERSI SAMPAH ORGANIK DENGAN METODE VERMICOMPOSTING (STUDI KASUS PT SARI ATER, SUBANG-JAWA BARAT)

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    Akumulasi sampah organik pada suatu industri pariwisata menimbulkan permasalahan estetik berupa bau dan ketidaknyamanan dipandang mata. Sampah organik ini dapat diolah dengan metode vermicomposting yang memanfaatkan pengaruh padat tebar cacing tanah jenis Lumbricus rubellus pada proses vermicomposting  terhadap konversi sampah restoran dan potongan rumput hingga menjadi kompos. Metode eksperimen dilakukan berdasarkan Rancangan Acak Lengkap (RAL) pada lima variasi perlakuan yang diulang lima kali, yaitu; Perlakuan A (tanpa cacing sebagai kontrol), B (dengan cacing sebanyak 1,5 kg/m2), C (dengan cacing 2 kg/m2),D (dengan cacing 2,5 kg/m2), dan E (dengan cacing 3 kg/m2). Selanjutnya dilakukan perhitungan persentase selisih kompos sebelum dan sesudah berlangsungnya proses komposting berdasarkan basis basah. Data dianalisis secara statistik dengan analisis keragaman, dilanjutkan dengan uji Duncan bila berpengaruh nyata. Hasil penelitian menunjukkan padat tebar yang diberikan dalam proses vermicomposting berpengaruh sangat nyata terhadap biokonversi sampah restoran dan potongan rumput hingga menjadi kompos.  Perlakuan C (jumlah cacing sebanyak 2 kg/m2) menghasilkan nilai biokonversi yang paling optimum dan  secara statistik berbeda sangat nyata dengan empat perlakuan lainnya. Kata kunci: sampah organik, vermicomposting, biokonvers
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