Linking gene expression to productivity to unravel long-and short-term responses of seagrasses exposed to CO2 in volcanic vents

Ocean acidification is a major threat for marine life but seagrasses are expected to benefit from high CO2. In situ (long-term) and transplanted (short-term) plant incubations of the seagrass Cymodocea nodosa were performed near and away the influence of volcanic CO2 vents at Vulcano Island to test the hypothesis of beneficial effects of CO2 on plant productivity. We relate, for the first time, the expression of photosynthetic, antioxidant and metal detoxification-related genes to net plant productivity (NPP). Results revealed a consistent pattern between gene expression and productivity indicating water origin as the main source of variability. However, the hypothesised beneficial effect of high CO2 around vents was not supported. We observed a consistent long-and short-term pattern of gene downregulation and 2.5-fold NPP decrease in plants incubated in water from the vents and a generalized upregulation and NPP increase in plants from the vent site incubated with water from the Reference site. Contrastingly, NPP of specimens experimentally exposed to a CO2 range significantly correlated with CO2 availability. The down-regulation of metal-related genes in C. nodosa leaves exposed to water from the venting site suggests that other factors than heavy metals, may be at play at Vulcano confounding the CO2 effects.ESF COST Action [ES0906]; Portuguese Foundation for Science and Technology (FCT) [PTDC/MAR-EST/3687/2012]; Italian MIUR Flagship project RITMARE (NRP); FCT [UID/Multi/04326/2013, SFRH/BPD/71129/2010, SFRH/BD/64590/2009]info:eu-repo/semantics/publishedVersio

Gene body DNA methylation in seagrasses: inter-and intraspecific differences and interaction with transcriptome plasticity under heat stress

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Investigating cellular stress response to heat stress in the seagrass Posidonia

Posidonia oceanica meadows are facing global threats mainly due to episodic heat waves. In a mesocosm experiment, we aimed at disentangling the molecular response of P. oceanica under increasing temperature (20 °C–32 °C). The experiment was carried out in spring, when heat waves can potentially occur and plants are putatively more sensitive to heat stress, since they are deprived in carbohydrates reserves after the cold winter months. We aimed to identify the activation of different phases of the cellular stress response (CSR) reaction and the responsive genes activated or repressed in heated plants. A molecular traffic light was proposed as a response model including green (protein folding and membrane protection), yellow (ubiquitination and proteolysis) and red (DNA repair and apoptosis) categories. Additionally, we estimated phenological trait variations to complement the information obtained from the molecular proxies of stress. Despite reduced leaf growth rate, heated plants did not exhibit signs of irreversible damage, probably underlying species pre-adaptation to warm and fluctuating regimes. Gene expression analyses revealed that molecular chaperoning, DNA repair and apoptosis inhibition processes related genes were the ones that mostly responded to high thermal stress and will be target of further investigation and in situ proofing for assessing their use as indicators of P. oceanica performance under sub-lethal heat stress