AKTIVITAS ANALGESIK EKSTRAK ETANOL DAUN SOYOGIK (Saurauia bracteosa DC) PADA TIKUS PUTIH JANTAN GALUR WISTAR

ABSTRACT  Soyogik leaves (Saurauia bracteosa DC) are plants that have flavonoid compounds that act as analgesics. This study aims to test the analgesic activity of soyogic leaf extract on male white rats Wistar strain using the thermic pain induction method. This research method is laboratory experimental, using male white rats (Rattus novergicus). The results obtained from the number of responses of mice between before and after giving the test solution. The treatment was initiated by thermal induction of pain in rats. Furthermore, the rat response was calculated, then given negative control (CMC), positive control (paracetamol), and soyogic leaf extract with a dose of 0.375 g / KgBB, 0.75 g / KgBB and 1.5 g / KgBB. The response of the mice after being given the treatment was seen from the 30th, 60th, 90th, and 120th minutes. Data were analyzed using the SPSS program, the real differences between the treatments were tested by one way ANOVA. The results showed that soyogic leaf extract with a dose of 0.375 g / KgBB, a dose of 0.75 g / KgBB and a dose of 1.5 g / KgBB have analgesic activity. Keywords: analgesics, Saurauia bracteosa DC, Rattus norvegicus  ABSTRAK Daun soyogik (Saurauia bracteosa DC) merupakan tanaman yang memiliki senyawa flavonoid yang berperan sebagai analgesik. Penelitian ini bertujuan untuk menguji aktivitas analgesik dari ekstrak daun soyogik pada tikus putih jantan galur wistar dengan metode induksi nyeri cara termik. Metode penelitian ini adalah eksperimental laboratorium, menggunakan tikus putih jantan (Rattus novergicus). Hasil penelitian diperoleh dari jumlah respon tikus antara sebelum dan sesudah pemberian larutan uji. Perlakuan diawali dengan induksi nyeri cara termik pada tikus. Selanjutnya dihitung jumlah respon tikus, kemudian diberikan kontrol negatif (CMC), kontrol positif (paracetamol), dan ekstrak daun soyogik dosis  0,375 g/KgBB, 0,75 g/KgBB dan 1,5 g/KgBB. Respon tikus setelah diberikan perlakuan dilihat mulai dari menit ke 30, 60, 90, dan 120. Data dianalisis menggunakan program SPSS, beda nyata antar perlakuan diuji dengan one way ANOVA. Hasil yang didapat menunukkan ekstrak daun soyogik dosis 0,375 g/KgBB, dosis 0,75 g/KgBB dan dosis 1,5 g/KgBB memiliki aktivitas analgesik.  Kata Kunci: analgesik, Saurauia bracteosa DC, Rattus norvegicu

ANALISIS BORAKS PADA TAHU YANG DIPRODUKSI DI KOTA MANADO

ABSTRACK Tofu is a food made by coagulating soy juice and then pressing the resulting curds into soft white blocks. The use of food additive in food process needs to be aware. Borax were restricted to be used, it cause brain damage, liver, and kidney. Prolonged use may cause death. The objectives of this research was to find out the presence of borax in tofu which produced in Manado city. Investigated tofu were sample A (Bahu), B (Batukota I), C (Batukota II), D (Kleak), and E (Pakowa). Borax were evaluated by flame test, curcuma paper color test, turmeric color test and spectrophotometry UV-vis. The results shows that there were five samples that did not contains borax using flame test, curcuma paper test, turmeric paper test and Spectrophotometry UV-Vis analysis.   Key words : Borax, Tofu, Qualitative analysis, Spectrophotometry UV-Vis, Manado

UJI FITOKIMIA DAN AKTIVITAS ANTIOKSIDAN DARI EKSTRAK ETANOL DAUN SOYOGIK (Saurauia bracteosa DC)

Soyogik (Saurauia bracteosa DC) is a plant in Actinidiaceae Families which isendemic in Indonesia, commonly found in Java and Bali. The aims of this research are toidentify the active compounds and determine the antioxidant activity of the leaf extractSoyogik. Soyogik leaves extracted by maceration and soxhlet using 70% ethanol as asolvent. The tests included quantitative test of antioxidant activity by DPPH method, andphytochemical test. The results show that maceration and soxhlet extract are containedphenolic compounds, steroids, flavonoids, and saponins. Both types of extract have powerfulantioxidant activity as showed on the IC50 values obtained. IC50 value of maceration andsoxhlet extract are 38,01 ppm and 28,18 ppm, respectively.Key word : phytochemical test, antioxidant, Saurauia bracteosa DC

UJI FITOKIMIA DAN AKTIVITAS ANTIOKSIDAN BUNGA SOYOGIK (Saurauia bracteosa DC)

ABSTRACTThese study aims to analyze the phytochemical compounds and the antioxidant activity of soyogik flowers. The study begins with a phytochemical test. Extraction of powder of soyogik flower by maceration using methanol. The methanol extract was determined for the total flavonoid content with AlCl3 method and was tested for the antioxidant activity with DPPH method. The results showed that soyogik flower containing the compounds of phenolic, flavonoids, alkaloids, steroids and saponins. The methanol extract also have total flavonoid content amounted to 11,140 mg/kg and antioxidant activity by the IC50 value amounted to 128,573 µg/mL.Keywords: Saurauia bracteosa DC., phytochemicals test, antioxidants, total flavonoid content.  ABSTRAKPenelitian ini bertujuan untuk menganalisis senyawa fitokimia dan mempelajari aktivitas antioksidan bunga soyogik. Penelitian dimulai dengan melakukan uji fitokimia. Ekstraksi serbuk bunga soyogik dengan cara maserasi menggunakan metanol. Ekstrak metanol kemudian ditentukan kandungan total flavonoid dengan metode AlCl3 dan diuji aktivitas antioksidannya dengan metode DPPH. Hasil penelitian menunjukkan bahwa bunga Soyogik mengandung senyawa fenolik, flavonoid, alkaloid, steroid dan saponin. Ekstrak metanol bunga Soyogik juga memiliki kandungan total flavonoid sebesar 11,140 mg/kg dan aktivitas antioksidan dengan nilai IC50 sebesar 128,573 µg/mL.Kata Kunci: Saurauia bracteosa DC., uji fitokimia, antioksidan, kandungan total flavonoid