EVOLUTIONARY RELATIONSHIP AND REPURPOSING OF SARS INHIBITORS AGAINST SURFACE GLYCOPROTEIN OF SARS-COV-2

Objective: Coronaviruses are a group of similar viruses which cause fatal infection and responsible for affecting the upper respiratory tract in many organisms. Throughout the time these viruses have been found to affect human life by causing major pandemics like SARS, MERS and COVID-19 due to their high rate of mutation and zoonotic transmission. Repurposing of a drug could be a solution for this challenge, as many previously available drugs hold great potential to act as a drug molecule. Interfering this interaction could be a potent mechanism to stop the viral infection and propagation. Methods: In the current study we have predicted the evolutionary relationship of nCoV using three viral proteins Nucleocapsid phosphoprotein, membrane glycoprotein and Envelop protein with accession number YP_009724397, YP_009724393 and YP_009724392 respectively. Phylogenetic tree was constructed and evaluated using the bootstrap method. Homology modeling and docking studies has been done to identify the interaction and binding affinity of SARS drugs. Results: Phylogenetic tree shows that Nucleocapsid phosphoprotein is originated from Hypsugo Bat Coronavirus, Membrane glycoprotein is originated from MERS Corona Virus and Envelop proteins have originated from Ferret coronavirus. From the docking result we concluded that Precose (glide score-8.372) shows that it has stable and strong interaction with Spike glycoprotein. Conclusion: Precose which is commonly known as Acarbose can act as a potential inhibitor for the spike glycoprotein. This paper described and highlighted the importance of repurposing of the previously available drug to act as a potent inhibitor in the newly discovered or novel diseases

Heat shock protein 70-2 (HSP70-2) overexpression in breast cancer

Supplementary Methods, Supplementary table, Supplementary Figure Legends. (DOCX 41 kb

The cross-sectional study of anxiety levels and ratio of severity of thirteen symptoms of anxiety among medical students

Background: Anxiety is defined as physical, behavioral, social and psychological response to treat self-concept characterized by subjective, consciously perceived feelings of tension. Nowadays anxiety is most commonly found among medical students. This study was conducted to find out the anxiety levels and ratio of severity of thirteen symptoms of anxiety.Methods: A questionnaire based study was conducted among 178 medical students which tests the level of anxiety and severity of symptoms of anxiety. The questionnaire used was hamilton anxiety rating scale (HAM-A).Results: Out of 178 students, 80 (44.94%) students scored mild anxiety levels, 63 (35.39%) students scored moderate anxiety levels and 35 (19.66%) students scored severe anxiety levels.Conclusions: Mild form of anxiety is much more common among medical students and majority of these medical students are females. Moreover, the symptoms of anxiety including tension, anxious mood, depressed mood, insomnia, fear and CVS symptoms appear with moderate severity in majority of medical students while on the other hand some symptoms including general somatic muscular and sensory symptoms, difficulties in concentration and memory, genitor-urinary symptoms, respiratory symptoms, GIT symptoms and other autonomic symptoms appear with least severity among majority of medical students

Supplementary_data_TUB – Supplemental material for Sperm associated antigen 9 (SPAG9) a promising therapeutic target of ovarian carcinoma

<p>Supplemental material, Supplementary_data_TUB for Sperm associated antigen 9 (SPAG9) a promising therapeutic target of ovarian carcinoma by Nirmala Jagadish, Rukhsar Fatima, Aditi Sharma, Sonika Devi, Vitusha Suri, Vikash Kumar and Anil Suri in Tumor Biology</p

Figure_S1_Supplementary_tub – Supplemental material for Sperm associated antigen 9 (SPAG9) a promising therapeutic target of ovarian carcinoma

<p>Supplemental material, Figure_S1_Supplementary_tub for Sperm associated antigen 9 (SPAG9) a promising therapeutic target of ovarian carcinoma by Nirmala Jagadish, Rukhsar Fatima, Aditi Sharma, Sonika Devi, Vitusha Suri, Vikash Kumar and Anil Suri in Tumor Biology</p

Experimental Incidence and Severity of Insect Pests of Brinjal (Solanum melongena)

The maximum population of Leucinodes orbonalis (fruit &amp; shoot borer) could be recorded at 40 SW or fourth week of September in 2014 and 41 SW 1st week of October in 2015 at a temperature range of 26-350C and RH 49-78% in season 2014 and 26-360C temperature and RH 48-81% in season 2015. At this temperature range and RH, 5.50/leaves in 2014 and 5.20/leaves in 2015. The maximum population of Amrasca biguttula biguttula was recorded at 40 SW or the fourth week of September in 2014 and 41 SW in 1st week of October in 2015 at a temperature range of 26-350C and RH 49-78% in season 2014 and 26-350C temperature and RH 48-81% in season 2015. At this temperature range and RH, 23.7/leaves in 2014 and 22.8/leaves in 2015. After that, it went on decreasing and became negligible at 49 SW

Additional file 6: Figure S5. of Heat shock protein 70-2 (HSP70-2) overexpression in breast cancer

Quantitative PCR analysis of various genes involved in different signaling cascades in breast cancer tumor xenograft. (PPTX 93 kb