miR-181a increases FoxO1 acetylation and promotes granulosa cell apoptosis via SIRT1 downregulation.

Oxidative stress impairs follicular development by inducing granulosa cell (GC) apoptosis, which involves enhancement of the transcriptional activity of the pro-apoptotic factor Forkhead box O1 (FoxO1). However, the mechanism by which oxidative stress promotes FoxO1 activity is still unclear. Here, we found that miR-181a was upregulated in hydrogen peroxide (

The relationship between work–family conflict and job satisfaction for preschool teachers in rural China: a moderated mediation model

BackgroundJob satisfaction for preschool teachers in rural areas has an important impact on their professional development, physical and mental health, and the development of preschool education. However, few studies have explored the factors that influence rural preschool teachers’ job satisfaction.PurposeThis study aims to examine the influence of rural preschool teachers’ work–family conflict on their job satisfaction, and the mediating effect of occupational identity, the moderating effect of social support.MethodParticipants included 3,065 rural preschool teachers from Zhejiang Province in mainland China. Teachers completed questionnaires on work–family conflict, occupational identity, job satisfaction, and social support. The correlation and moderated mediation analyses were conducted using SPSS PROCESS.Results(1) work–family conflict is associated with poorer job satisfaction in preschool teachers; (2) occupational identity mediates the relationship between work–family conflict and job satisfaction; and (3) a high level of social support alleviates the negative influence of work–family conflict on job satisfaction and promotes the positive effect of occupational identity on job satisfaction.ConclusionThe study revealed the negative impact of work–family conflict on preschool teachers’ job satisfaction, and the protecting effect of social support, which has important implications for improving teachers’ future job satisfaction

Different Angiogenic Potentials of Mesenchymal Stem Cells Derived from Umbilical Artery, Umbilical Vein, and Wharton's Jelly

Human mesenchymal stem cells derived from the umbilical cord (UC) are a favorable source for allogeneic cell therapy. Here, we successfully isolated the stem cells derived from three different compartments of the human UC, including perivascular stem cells derived from umbilical arteries (UCA-PSCs), perivascular stem cells derived from umbilical vein (UCV-PSCs), and mesenchymal stem cells derived from Wharton’s jelly (WJ-MSCs). These cells had the similar phenotype and differentiation potential toward adipocytes, osteoblasts, and neuron-like cells. However, UCA-PSCs and UCV-PSCs had more CD146+ cells than WJ-MSCs (P<0.05). Tube formation assay in vitro showed the largest number of tube-like structures and branch points in UCA-PSCs among the three stem cells. Additionally, the total tube length in UCA-PSCs and UCV-PSCs was significantly longer than in WJ-MSCs (P<0.01). Microarray, qRT-PCR, and Western blot analysis showed that UCA-PSCs had the highest expression of the Notch ligand Jagged1 (JAG1), which is crucial for blood vessel maturation. Knockdown of Jagged1 significantly impaired the angiogenesis in UCA-PSCs. In summary, UCA-PSCs are promising cell populations for clinical use in ischemic diseases