2D2D HILIC-ELSD/UPLC-Q-TOF-MS Method for Acquiring Phospholipid Profiles and the Application in Caenorhabditis elegans

Phospholipids are the main constituent of cellular membranes and have recently been identified to have diagnostic value as biomarkers for many diseases. Accordingly, much emphasis is now laid on developing optimal analytical techniques for the phospholipid profiles of various biological samples. In the present study, different classes of phospholipids are first separated by optimized hydrophilic interaction chromatography with evaporative light scattering detector (HILIC-ELSD). The phospholipids in each class are then identified by ultraperformance liquid chromatography-quadrupole time-of-flight mass spectrometry (UPLC-Q-TOF-MS). Validation results confirm that this approach meets the requirements of quantitative analysis. Finally, the approach is adopted to analyze the phospholipid profiles in Caenorhabditis elegans. A total of 111 phospholipid species is identified according to the mass fragments. Major fatty acyl chains in phospholipids are found to be formed by oleic acid (C18:1), arachidonic acid (C20:4), and eicosapentaenoic acid (C20:5). Overall, this study improves current knowledge on analytical techniques of the phospholipid composition in C. elegans and provides a basis for future lipidomics research. Practical applications: Phospholipids reportedly play a crucial role in the development of many diseases. Until now, only a small portion of phospholipids in Caenorhabditis elegans has been reported by using one-dimensional analysis strategy. The offline 2D2D liquid chromatography method developed in this study identifies 111 phospholipid species in Caenorhabditis elegans. The obtained phospholipid profiles complement the lipid database of Caenorhabditis elegans. The study also provides the basis for the future development of a 2D online approach

Effects of Medium- and Long-Chain Structured Triacylglycerol on the Therapeutic Efficacy of Vitamin D on Ulcerative Colitis:A Consideration for Efficient Lipid Delivery Systems

Due to intestinal malabsorption and poor water solubility, vitamin D (VitD) deficiency in ulcerative colitis (UC) continues to increase. Medium- and long-chain triacylglycerols (MLCT), as novel lipids, have been widely applied in the field of functional food and medicine nutrition. Our previous studies showed that the difference in MLCT structure could affect VitD bioaccessibility in vitro. In this study, our results further indicate that, although identical in fatty acid composition, structured triacylglycerol (STG) had a higher VitD bioavailability (AUC = 15470.81 μg/L × h) and metabolism efficacy [s-25(OH)D, p &lt; 0.05] than physical mixtures of triacylglycerol (PM), which further affect the amelioration efficiency in UC mice. Compared with PM, the damage of colonic tissues, intestinal barrier proteins, and inflammatory cytokines in STG showed better amelioration at the same dose of VitD. This study provides a comprehensive understanding of the mechanism of nutrients in different carriers and a solution for developing nutrients with high absorption efficiency.</p

Effects of Medium- and Long-Chain Structured Triacylglycerol on the Therapeutic Efficacy of Vitamin D on Ulcerative Colitis:A Consideration for Efficient Lipid Delivery Systems

Due to intestinal malabsorption and poor water solubility, vitamin D (VitD) deficiency in ulcerative colitis (UC) continues to increase. Medium- and long-chain triacylglycerols (MLCT), as novel lipids, have been widely applied in the field of functional food and medicine nutrition. Our previous studies showed that the difference in MLCT structure could affect VitD bioaccessibility in vitro. In this study, our results further indicate that, although identical in fatty acid composition, structured triacylglycerol (STG) had a higher VitD bioavailability (AUC = 15470.81 μg/L × h) and metabolism efficacy [s-25(OH)D, p &lt; 0.05] than physical mixtures of triacylglycerol (PM), which further affect the amelioration efficiency in UC mice. Compared with PM, the damage of colonic tissues, intestinal barrier proteins, and inflammatory cytokines in STG showed better amelioration at the same dose of VitD. This study provides a comprehensive understanding of the mechanism of nutrients in different carriers and a solution for developing nutrients with high absorption efficiency.</p

The Mediating Effect of Depression on the Relation Between Interpersonal Needs and Suicidal Ideation Among Chinese Transgender Women

Background: Transgender women are at high risk of depression and suicidal ideation. The interpersonal theory of suicide proposes that suicidal ideation could be a consequence of high interpersonal needs (thwarted belongingness and perceived burdensomeness). The current study tests this theory and investigates whether depression could mediate the relationship between interpersonal needs and suicidal ideation among transgender women in Shenyang, China. Methods: A total of 198 transgender women were recruited by snowball sampling. A cross-sectional study was conducted through a structured questionnaire. Suicidal ideation, depression, and interpersonal needs were assessed. Path analysis was used to carry out the research goals and the mediating effect of depression was tested. Results: There were nearly 37% of the participants reported lifetime suicidal ideation. Suicidal ideation was positively correlated with thwarted belongingness (t = −5.53, p \u3c 0.01) and perceived burdensomeness (t = −5.02, p \u3c 0.01). The direct effect from thwarted belongingness to suicidal ideation via depression was statistically significant (Std. β = 0.232, p \u3c 0.01). Depression could also mediate the indirect path from perceived burdensomeness to suicidal ideation through depression (Std. β = 0.222, p \u3c 0.01) although the direct path between them was not significant (Std. β = 0.046, p = 0.693). Conclusions: Depression fully mediated the relationship between perceived burdensomeness and suicidal ideation, and partially mediate the relationship between thwarted belongingness and suicidal ideation. To reduce the risk of suicidal ideation among transgender women, interventions targeting thwarted belongingness, perceived burdensomeness, and depression are needed

Performance of fast-ion loss diagnostic on EAST

The scintillator-based detector for fast-ion loss measurements has been installed on EAST. To obtain high temporal resolution for fast-ion loss diagnostics, fast photomultiplier tube systems have been developed which can supply the complementary measurements to the previous image system with good energy and pitch resolution by using a CCD camera. By applying the rotatable platform, the prompt losses of beam-ions can be measured in normal and reverse magnetic field. The thick-target bremsstrahlung occurring in the stainless steel shield with energetic electrons can produce X-rays, which will strike on the scintillator based detector. To understand this interference on fast-ion loss signals, the effects of energetic electrons on the scintillator-based detector are studied, including runaway electrons in the plasma ramping-up phase and fast electrons accelerated by the lower hybrid wave

Gender-Specific and U-Shaped Relationship Between Serum Uric Acid and All-Cause Mortality Among Chinese Older Adults: A National Population-Based Longitudinal Study

Objectives: This study aimed to prospectively investigate gender-specific relationship between hyperuricemia and all-cause mortality among Chinese older adults.Methods: The study was based on the Chinese Longitudinal Healthy Longevity Survey (CLHLS) 2008–2018, a prospective nationwide cohort of older adults in China. Multivariate Cox proportional hazards models were used to estimate hazard ratios (HRs) and 95% CIs for all-cause mortality. Restricted cubic splines (RCS) were conducted to explore the dose-response relationship between SUA levels and all-cause mortality.Results: For older women, compared to the participants in the third quartile of SUA level, those in the highest quartile of SUA was associated with significantly higher risk of all-cause mortality in the fully adjusted model (HR: 1.41, 95% CI: 1.03–1.92). No significant associations between SUA levels and all-cause mortality were observed in older men. The present study further found a U-shaped non-linear relationship between SUA levels and all-cause mortality in both sexes of older population (P for non-linear &lt;0.05).Conclusions: This study provided prospective epidemiological evidence for the predictive role of SUA on all-cause mortality among the Chinese aging population over 10 years of follow-up, while revealing considerable gender-related differences

The prevalence of childhood sexual experiences and intimate partner violence among transgender women in China: Risk factors for lifetime suicidal ideation

ObjectiveSeveral studies highlighted childhood sexual experiences (CSEs) and intimate partner violence (IPV) as risk factors that affected lifetime suicidal ideation. TW had higher rates of CSEs and IPV than cisgender people. The aim of this study was to comprehensively assess the prevalence of CSEs and IPV among TW and their association with lifetime suicidal ideation.MethodsA cross-sectional survey was conducted among 247 TW in Shenyang and Kunming, China, from April to September 2018. CSEs, IPV, and lifetime suicidal ideation were assessed. Logistic regression models were used to examine the association between self-reported CSEs under 18 years of age, IPV in adulthood, and lifetime suicidal ideation.ResultsIn the study, 14.2% (35/247) of the sample participants reported CSEs under 18 years of age; 44.9% (111/247) reported experiencing IPV in adulthood, including 18.6% (44/247) of physical IPV, 27.1% (67/247) of trans-specific identity IPV, 31.6% (78/247) of verbal IPV, and 19.4% (48/247) of sexual IPV; and 26.3% (65/247) had thought about attempting suicide at least one time. CSEs and any form of IPV were significantly associated with suicidal ideation in this sample population. A final stepwise multivariate logistic regression model found that both physical and verbal IPVs were significantly associated with suicidal ideation when controlling for other factors (ORm1 = 2.58, 95% confidence interval (CI) = 1.163–5.724; ORm2 = 2.72, 95% CI = 1.334–5.547).ConclusionsThe findings highlight the effects of CSEs and IPV among TW and suggest the need for research on suicide in the future. Suicide prevention efforts for this invisible and vulnerable population should focus on those with physical and verbal IPV

Unraveling the Photoluminescent Properties of Sb-Doped Cd-Based Inorganic Halides: A First-Principles Study

Sb-doped Cd-based inorganic halides, with varying connections of CdCl6 octahedra ranging from 0D to 3D, exhibit a variety of photoluminescent properties. Single-band emission is observed in Sb-doped Rb4CdCl6 (0D) and Cs2CdCl4 (2D), while dual-band emission is seen in Sb-doped RbCdCl3 (1D) and CsCdCl3 (3D). Density-functional-based first-principles calculations were conducted. The results reveal that cation vacancies, acting as charge compensators, influence the luminescence properties of dopant centers. In CsCdCl3, the local cation vacancy VCd″ for Sb3+ at the Cd2+ site ([Sb□Cl9]6–) significantly modifies the photoluminescence property, accounting for the observed dual-band emission alongside the nonlocal compensation case. This effect is insignificant in Sb-doped Rb4CdCl6, RbCdCl3, and Cs2CdCl4, due to the large distances or high formation energies of Cd vacancies in these hosts. However, in Sb-doped RbCdCl3, two different potential energy minima, one that involves typical structure relaxation and the other that is off-center, lead to the observed dual-band emission. Furthermore, the shift of the charge transition level illustrates the different temperature dependences of the dual-band emission caused by the charge-compensating point defects. These insights not only enhance our understanding of luminescent materials based on halides containing ns2 dopants but also provide valuable guidance for the design and optimization of luminescent materials

An Identification and Expression Analysis of the ABCG Genes Related to Benzaldehyde Transportation among Three Prunus Species

The plants of Prunus mostly bloom in early spring, and the flowers of various species possess their individual floral scent characteristics; Prunus mume, especially, can volatilize a large amount of benzenoid compounds into the air during the flowering phase. In order to elucidate the molecular basis of the differences in the volatile capacity of aromatic substances among Prunus flowers, the endogenous and the headspace volatile components and the expression of ABCG genes were studied among P. mume, P. armeniaca, and P. persica. We detected the floral components in the three species by gas chromatography-mass spectrometry (GC-MS), and we found that benzaldehyde was the key component. Meanwhile, the volatilization efficiency of benzaldehyde in P. mume and P. armeniaca were much higher than that in P. persica. Furthermore, 130, 135, and 133 ABC family members from P. mume, P. armeniaca, and P. persica were identified, respectively. WGCNA analysis demonstrated that candidate ABCG genes were positively correlated with benzaldehyde volatilization efficiency. Moreover, quantitative Real-time PCR indicated that ABCG17 was more likely to be involved in the transmembrane transport of benzaldehyde. This study aimed to provide a theoretical basis for elucidating the transmembrane transport of benzaldehyde and to further the valuable information for fragrant flower breeding in Prunus