Subcellular localization of APMCF1 and its biological significance of expression pattern in normal and malignant human tissues

<p>Abstract</p> <p>Background</p> <p>APMCF1 is a novel human gene first cloned from apoptotic MCF-7 cells. Our previous study found ectogenic APMCF1 could induce G1 arrest in hepatocarcinoma cell line HHCC. In order to search its broad expression profile for further understanding of its mechanism in tumor, we investigated a subcellular location of APMCF1 and performed an immunohistochemistry study including various tumor and normal tissues. Discovery from the expression characterization of AMPCF1 may have applicability in the analysis of its biological function in tumor.</p> <p>Methods</p> <p>We investigated subcellular localization of APMCF1 by transient transfection in green monkey kidney epithelial cells (COS-7) with a fusion protein vector pEGFP-APMCF1 and detected expression profile in a broad range of normal and malignant human tissues via tissue microarray (TMA) by immunohistochemistry with polyclonal antibody first produced in our laboratory.</p> <p>Results</p> <p>EGFP-APMCF1 was generally localized in the cytoplasm of COS-7 cell. Positive staining of APMCF1 was found in liver, lung, breast, colon, stomach, esophagus and testis, exhibited a ubiquitous expression pattern while its expression was up-regulated in tumor tissues compared with corresponding normal tissues. Normal brain neuron cells also showed expression of APMCF1, but negative in gliocyte cells and glioma. Both the normal and tumor tissues of ovary were absent of APMCF1 expression. Positive immunostaining for APMCF1 with large samples in liver, colon, esophagus, lung and breast carcinomas were 96% (51/53), 80% (44/55), 57% (30/53), 58% (33/57) and 34% (16/47) respectively.</p> <p>Conclusion</p> <p>These results revealed a cytoplastic expression pattern of APMCF1 and up-regulated in tumour tissues suggesting APMCF1 may have potential relationship with oncogenesis. The data presented should serve as a useful reference for further studies of APMCF1 functions in tumorigenesis and might provide a potential anti-tumor target.</p

Retrospective evaluation of whole exome and genome mutation calls in 746 cancer samples

Funder: NCI U24CA211006Abstract: The Cancer Genome Atlas (TCGA) and International Cancer Genome Consortium (ICGC) curated consensus somatic mutation calls using whole exome sequencing (WES) and whole genome sequencing (WGS), respectively. Here, as part of the ICGC/TCGA Pan-Cancer Analysis of Whole Genomes (PCAWG) Consortium, which aggregated whole genome sequencing data from 2,658 cancers across 38 tumour types, we compare WES and WGS side-by-side from 746 TCGA samples, finding that ~80% of mutations overlap in covered exonic regions. We estimate that low variant allele fraction (VAF < 15%) and clonal heterogeneity contribute up to 68% of private WGS mutations and 71% of private WES mutations. We observe that ~30% of private WGS mutations trace to mutations identified by a single variant caller in WES consensus efforts. WGS captures both ~50% more variation in exonic regions and un-observed mutations in loci with variable GC-content. Together, our analysis highlights technological divergences between two reproducible somatic variant detection efforts

Fe-Mn based catalytic ceramic filters with SO2 resistance for NH3-SCR at low temperature

The Fe-Mn based catalyst was loaded on porous ceramic filter with impregnation method to prepare a catalytic ceramic filter which integrates the denitration and dust removal at low temperature. The effect of the Fe/Mn ratio and doping Sm on the catalytic performance of Fe-Mn based catalyst were studied. In the temperature range of 120 °C–220 °C, the Fe _2 Mn _1 Sm _0.2 O _x catalytic filter exhibited over 90% NO removal efficiency. When 100 ppm SO _2 and 7vol.% H _2 O in the flue gas, the NO removal efficiency still maintained above 89% at 200 °C. These results proved that the Fe _2 Mn _1 Sm _0.2 O _x catalytic filter has excellent catalytic performance and excellent H _2 O and SO _2 resistance at low temperature. In addition, the dust collection efficiency reached 99.99%. The NH _3 -TPD, H _2 -TPR and XPS confirmed that the Fe _2 Mn _1 O _x catalysts doped with Sm have stronger surface acid, more surface absorbed oxygen and more oxygen vacancy, which lead to higher NH _3 -SCR activity and better SO _2 and H _2 O resistance

Catalytic filter for the removal of dust and NOx at low temperature

The catalytic filter was fabricated by supporting selective catalytic reduction (SCR) catalyst on the low-density ceramic (LDC) for the removal of dust and nitrogen oxides (NO _x ) in the flue gases at relative low temperature. MnO _x –ZrO _2 /TiO _2 catalyst was selected as SCR catalyst. The NO _x and dust removal efficiency, filter resistance, regeneration performance and anti-sulfur performance were investigated. The result showed that the NO _x removal efficiency at 180°C reached 98.4% (1 m/min filtration velocity) for 6 wt% MnO _x –ZrO _2 /TiO _2 catalytic filter with Mn/Zr molar ratio of 2. Furthermore, MnO _x –ZrO _2 /TiO _2 catalytic filter performed good anti-sulfur performance. In the presence of 10 vol% water vapor and 100 ppm SO _2 at 180 °C, the NO _x removal efficiency for MnO _x –ZrO _2 /TiO _2 catalytic filter could retain up to 83.2% and it could recover to 91.8% when the water vapor and SO _2 were cut off. MnO _x –ZrO _2 /TiO _2 catalytic filter showed the high dust removal efficiency of 99.99% and the low filter resistance of less than 200 Pa. The filter resistance of MnO _x –ZrO _2 /TiO _2 catalytic filter could maintain 235.7 Pa after 200 times pulse blowback. The result illustrated that MnO _x –ZrO _2 /TiO _2 catalytic filter showed good regeneration performance

Study on the Creep-Sliding Mechanism of the Giant Xiongba Ancient Landslide Based on the SBAS-InSAR Method, Tibetan Plateau, China

The geohazards associated with strongly deformed and reactivated large-scale ancient landslide are analyzed through a study of the Xiongba ancient landslide. The SBAS-InSAR method, combined with remote sensing interpretation, was used to obtain the Xiongba ancient landslide surface deformation characteristics, on the western bank of the Jinsha River, during the period from October 2017 to June 2020. Two large strong deformation zones were discovered in this study, H1 and H2, which were located at the front edge of the Xiongba landslide. The maximum cumulative deformation in the H1 deformation zone was approximately 204 mm, and the deformation in the H2 deformation zone was approximately 302 mm. Influenced by the Jinsha River erosion, the Baige landslide-dammed lake-dam breakage-debris (LDLDB) flow/flood hazard chains, which occurred 75 km upstream reaches in October and November 2018, and the erosion of the foot of the Xiongba ancient landslide foot resulted in notably enhanced deformation. The creep rate in the H1 deformation zone was 14~16 times that before the Baige landslide hazard chains occurred, and the hazard chains caused sliding in the H2 zone. The Xiongba ancient landslide is undergoing retrogressive reactivation. The Xiongba ancient landslide is currently experiencing continuously creep-sliding, and the deformation rate in some areas is accelerating, which may induce a large-scale reactivation of the Xiongba ancient landslide and an LDLDB hazard chain

The neuropathological mechanism of EV-A71 infection attributes to inflammatory pryoptosis and viral replication via activating the hsa_circ_0045431/ hsa_miR_584/NLRP3 regulatory axis

Neuropathological damage has been considered to be the main cause of death from EV-A71 infection, but the underlying mechanism has not been elucidated. Pyroptosis, a new form of inflammatory programmed cell death, has been verified to be involved in the pathogenesis of various viruses. circRNAs are a novel type of endogenous noncoding RNA gaining research interest in recent years, especially their special roles in the process of virus infection. Thus, in this study, we combined EV-A71, pyroptosis and circRNA to find a breakthrough in the pathogenesis of EV-A71 infection. Firstly, whether EV-A71 infection leaded to pyroptosis formation was examined by a series detection of cell death, cell viability, LDH release, caspase 1 activity, the expression levels of pyroptosis-related molecules and the concentrations of IL-1β and IL-18. Secondly, high-throughput sequencing of circRNAs was carried out to excavate the circRNA-miRNA-mRNA regulatory axis which might be associated with pyroptosis formation. Finally, the gain- and loss-of-functional experiments were further conducted to identify their functions. Our results showed that EV-A71 infection caused pyroptosis formation in SH-SY5Y cells. The circRNA sequencing analyzed the differentially expressed circRNAs and their possible functions. It was found that the hsa_circ_0045431/hsa_miR_584/NLRP3 regulatory axis might be involved in pyroptosis formation during EV-A71 infection. Then, hsa_circ_0045431 sponged hsa_miR_584 and hsa_miR_584 directly targeted NLRP3 were validated by IF, dual-luciferase, qRT-PCR and WB assays. Functional experiments were performed to further uncover that the up-regulation of hsa_circ_0045431 and NLRP3 promoted the inflammatory pyroptosis and viral replication, while the up-regulation of hsa_miR_584 suppressed the inflammatory pyroptosis and viral replication, and vice versa. Collectively, our study demystified that EV-A71 infection induced pyroptosis formation by activating hsa_circ_0045431/hsa_miR_584/NLRP3 regulatory axis, which could further effect viral replication. These findings provided novel insights into the pathogenesis of EV-A71 infection, and meanwhile revealed that the hsa_circ_0045431/ hsa_miR_584/NLRP3 regulatory axis can serve as a potential biological therapeutic target for EV-A71 infection

Metagenomics of gut microbiome for migratory seagulls in Kunming city revealed the potential public risk to human health

Abstract Background Seagull as a migratory wild bird has become most popular species in southwest China since 1980s. Previously, we analyzed the gut microbiota and intestinal pathogenic bacteria configuration for this species by using 16S rRNA sequencing and culture methods. To continue in-depth research on the gut microbiome of migratory seagulls, the metagenomics, DNA virome and RNA virome were both investigated for their gut microbial communities of abundance and diversity in this study. Results The metagenomics results showed 99.72% of total species was bacteria, followed by viruses, fungi, archaea and eukaryota. In particular, Shigella sonnei, Escherichia albertii, Klebsiella pneumonia, Salmonella enterica and Shigella flexneri were the top distributed taxa at species level. PCoA, NMDS, and statistics indicated some drug resistant genes, such as adeL, evgS, tetA, PmrF, and evgA accumulated as time went by from November to January of the next year, and most of these genes were antibiotic efflux. DNA virome composition demonstrated that Caudovirales was the most abundance virus, followed by Cirlivirales, Geplafuvirales, Petitvirales and Piccovirales. Most of these phages corresponded to Enterobacteriaceae and Campylobacteriaceae bacterial hosts respectively. Caliciviridae, Coronaviridae and Picornaviridae were the top distributed RNA virome at family level of this migratory animal. Phylogenetic analysis indicated the sequences of contigs of Gammacoronavirus and Deltacoronavirus had highly similarity with some coronavirus references. Conclusions In general, the characteristics of gut microbiome of migratory seagulls were closely related to human activities, and multiomics still revealed the potential public risk to human health