Filter Pruning via Filters Similarity in Consecutive Layers

Filter pruning is widely adopted to compress and accelerate the Convolutional Neural Networks (CNNs), but most previous works ignore the relationship between filters and channels in different layers. Processing each layer independently fails to utilize the collaborative relationship across layers. In this paper, we intuitively propose a novel pruning method by explicitly leveraging the Filters Similarity in Consecutive Layers (FSCL). FSCL compresses models by pruning filters whose corresponding features are more worthless in the model. The extensive experiments demonstrate the effectiveness of FSCL, and it yields remarkable improvement over state-of-the-art on accuracy, FLOPs and parameter reduction on several benchmark models and datasets.Comment: Accepted by ICASSP 2023 (oral

miR-638 is a new biomarker for outcome prediction of non-small cell lung cancer patients receiving chemotherapy.

MicroRNAs (miRNAs), a class of small non-coding RNAs, mediate gene expression by either cleaving target mRNAs or inhibiting their translation. They have key roles in the tumorigenesis of several cancers, including non-small cell lung cancer (NSCLC). The aim of this study was to investigate the clinical significance of miR-638 in the evaluation of NSCLC patient prognosis in response to chemotherapy. First, we detected miR-638 expression levels in vitro in the culture supernatants of the NSCLC cell line SPC-A1 treated with cisplatin, as well as the apoptosis rates of SPC-A1. Second, serum miR-638 expression levels were detected in vivo by using nude mice xenograft models bearing SPC-A1 with and without cisplatin treatment. In the clinic, the serum miR-638 levels of 200 cases of NSCLC patients before and after chemotherapy were determined by quantitative real-time PCR, and the associations of clinicopathological features with miR-638 expression patterns after chemotherapy were analyzed. Our data helped in demonstrating that cisplatin induced apoptosis of the SPC-A1 cells in a dose- and time-dependent manner accompanied by increased miR-638 expression levels in the culture supernatants. In vivo data further revealed that cisplatin induced miR-638 upregulation in the serum derived from mice xenograft models, and in NSCLC patient sera, miR-638 expression patterns after chemotherapy significantly correlated with lymph node metastasis. Moreover, survival analyses revealed that patients who had increased miR-638 levels after chemotherapy showed significantly longer survival time than those who had decreased miR-638 levels. Our findings suggest that serum miR-638 levels are associated with the survival of NSCLC patients and may be considered a potential independent predictor for NSCLC prognosis

Magnesium Lithospermate B Protects Cardiomyocytes from Ischemic Injury Via Inhibition of TAB1–p38 Apoptosis Signaling

Danshen has been used in traditional Chinese medicine for hundreds of years to treat cardiovascular diseases. However, its precise cardioprotective components and the underlying mechanism are still unclear. In the present study, we demonstrated that in a rat model of acute myocardial infarction, the treatment with magnesium lithospermate B (MLB), the representative component of phenolic acids in Danshen, significantly reduced the infarct size and the blood lactate dehydrogenase level. In contrast, tanshinone IIA, the representative component of lipophilic tanshinones in Danshen, had no such protective effects. Moreover, in the simulated ischemia cell model, MLB treatment considerably increased the cell viability and reduced the sub-G1 population and the apoptotic nuclei, indicating its anti-apoptotic effect. Further mechanism study revealed that the ischemia-induced p38 phosphorylation was abolished by MLB treatment. Interestingly, MLB specifically inhibited the TGFβ-activated protein kinase 1-binding protein 1 (TAB1) mediated p38 phosphorylation through disrupting the interaction between TAB1 and p38, but it did not affect the mitogen-activated protein kinase 3/6 mediated p38 phosphorylation. In conclusion, the present study identifies MLB as an active component of Danshen in protecting cardiomyocytes from ischemic injury through specific inhibition of TAB1–p38 apoptosis signaling. These results indicate TAB1–p38 interaction as a putative drug target in treating ischemic heart diseases

Effects of fundamental movement skills on health-related quality of life in Chinese school-age children: the mediating role of physical fitness level

BackgroundThe primary purpose of this study is to analyze the relationship between school-age children’s fundamental movement skills (FMS), physical fitness levels, and the health-related quality of life (HRQoL); To explore the mediating role of physical fitness levels between school-age children’s FMS and HRQoL.MethodsIn the cross-sectional survey in 2021, 334 school-age children aged 6–10 (8.20 ± 1.16) were recruited from primary schools in Jinhua City, Zhejiang Province, China. Test of Gross Motor Development 2 (TGMD-2), National Standards for Students’ Physical Health, and Pediatric Quality of Life Inventory TM Version 4.0 (PedsQL™ 4.0) were used to investigate the FMS, physical fitness level, and HRQoL of school-age children. Hierarchical regression was used to analyze the relationship among FMS, physical fitness levels, and HRQoL. Bootstrap is used to evaluate the mediating role of physical fitness levels in the relationship between FMS and HRQoL.ResultsThe higher the FMS and physical fitness, the higher the school-age children’s HRQoL, physical functioning, social functioning, and school functioning (r = 0.244–0.301, p < 0.01). In addition, developing children’s FMS promotes physical fitness levels (r = 0.358, p < 0.01). The regression analysis results of controlling gender, age, and body mass index z (BMI-z) scores showed that FMS significantly positively predicted the physical functioning (β = 0.319, p < 0.01), social functioning (β = 0.425, p < 0.01), and school functioning (β = 0.333, p < 0.01) of school-age children. When the physical fitness level enters the regression equation, the absolute value of the regression coefficient of FMS decreases. However, it can still significantly predict the physical functioning (β = 0.211, p < 0.01) and school functioning (β = 0.142, p < 0.05) of school-age children. Simple intermediary analysis shows that physical fitness level plays an intermediary role between FMS, physical functioning (indirect effect = 0.089 [95% Confidence interval (CI) = 0.015,0.195]), and school functioning (indirect effect = 0.065 [95% CI = 0.007,0.150]).ConclusionThis study shows that physical fitness levels mediate the relationship between FMS and HRQoL. Encouraging the development of FMS and promoting physical fitness levels of school-age children can effectively improve the HRQoL of school-age children

Stavudine exposure results in developmental abnormalities by causing DNA damage, inhibiting cell proliferation and inducing apoptosis in mouse embryos

Stavudine is an anti-AIDS drug widely used to prevent HIV transmission from pregnant mothers to the fetuses in underdeveloped countries for its low price. However, there is still a controversy on whether stavudine affects embryo development. In the current study, embryotoxicity of stavudine was evaluated using cultured mouse embryos with the concentrations: 5, 10, 15 μM and vehicle control. The data indicated that the effect of stavudine was dose-dependent at early neurogenesis. Stavudine exposure reduced somite numbers, yolk sac diameter, crown-rump length, and increased the rate of embryonic degeneration compared with the control. We chose the lowest but clearly toxic concentration: 5 μM to investigate the molecular mechanisms of the damage. At the molecular level, stavudine produced DNA damage, increased the levels of the phospho-CHK1 and cleaved-caspase-3, and decreased the expression level of proliferating cell nuclear antigen. These changes indicated that stavudine caused a coordinated DNA damage response, inhibited cell proliferation, and induced apoptosis in the embryos. Collectively these results suggest that stavudine exposure disturbs the embryonic development, and its use in pregnant mothers should be re-examined

Interdecadal variability of the eastward current in the South China Sea associated with the summer Asian monsoon

Author Posting. © American Meteorological Society, 2010. This article is posted here by permission of American Meteorological Society for personal use, not for redistribution. The definitive version was published in Journal of Climate 23 (2010): 6115-6123, doi:10.1175/2010JCLI3607.1.Based on the Simple Ocean Data Assimilation (SODA) dataset and three types of Sverdrup streamfunction, an interdecadal variability of the eastward current in the middle South China Sea (SCS) during summer is identified. Both the pattern and strength of the summer Asian monsoon wind stress curl over the SCS contribute to the interdecadal variability of this current. From 1960 to 1979, the monsoon intensified and the zero wind stress curl line shifted southward. Both the core of positive wind stress curl in the northern SCS and the negative curl in the southern SCS moved southward and thus induced a southward shift of both the southern anticyclonic and northern cyclonic gyres, resulting in a southward displacement of the eastward current associated with these two gyres. In the meantime, the southern (northern) SCS anticyclonic (cyclonic) ocean gyre weakened (strengthened) and therefore also induced the southward shift of the eastward current near the intergyre boundary. In contrast, the eastward current shifted northward from 1980 to 1998 because the monsoon relaxed and the zero wind stress curl line shifted northward. After 1998, the eastward jet moved southward again as the zero wind stress curl line shifted southward and the SCS monsoon strengthened. The eastward current identified from the baroclinic streamfunction moved about 1.7° more southward than that from the barotropic streamfunction, indicating that the meridional position of the eastward current is depth dependent.This study was supported by the National BasicResearch Program (Grant 2007CB816003) and the National Natural Science Foundation of China (Grants 40976017, 40730843, and 40876004)

Integrated gene-based and pathway analyses using UK Biobank data identify novel genes for chronic respiratory diseases

BackgroundChronic respiratory diseases have become a non-negligible cause of death globally. Although smoking and environmental exposures are primary risk factors for chronic respiratory diseases, genetic factors also play an important role in determining individual’s susceptibility to diseases. Here we performed integrated gene-based and pathway analyses to systematically illuminate the heritable characteristics of chronic respiratory diseases.MethodsUK (United Kingdom) Biobank is a very large, population-based prospective study with over 500,000 participants, established to allow detailed investigations of the genetic and nongenetic determinants of the diseases. Utilizing the GWAS-summarized data downloaded from UK Biobank, we conducted gene-based analysis to obtain associations of susceptibility genes with asthma, chronic obstructive pulmonary disease (COPD) and pneumonia using FUSION and MAGMA software. Across the identified susceptibility regions, functional annotation integrating multiple functional data sources was performed to explore potential regulatory mechanisms with INQUISIT algorithm. To further detect the biological process involved in the development of chronic respiratory diseases, we undertook pathway enrichment analysis with the R package (clusterProfiler).ResultsA total of 195 susceptibility genes were identified significantly associated with chronic respiratory diseases (Pbonferroni < 0.05), and 24/195 located out of known susceptibility regions (e.g. WDPCP in 2p15). Within the identified susceptibility regions, functional annotation revealed an aggregation of credible variants in promoter-like and enhancer-like histone modification regions and such regulatory mechanisms were specific to lung tissues. Furthermore, 110 genes with INQUISIT score ≥1 may influence diseases susceptibility through exerting effects on coding sequences, proximal promoter and distal enhancer regulations. Pathway enrichment results showed that these genes were enriched in immune-related processes and nicotinic acetylcholine receptors pathways.ConclusionsThis study implemented an integrated gene-based and pathway strategy to explore the underlying biological mechanisms and our findings may serve as promising targets for future clinical treatments of chronic respiratory diseases

Smart Hydrogel Grating Immunosensors for Highly Selective and Sensitive Detection of Human-IgG

This document is the Accepted Manuscript version of a Published Work that appeared in final form in [Industrial & Engineering Chemistry Research], copyright © American Chemical Society after peer review and technical editing by the publisher. To access the final edited and published work see [https://pubs.acs.org/doi/10.1021/acs.iecr.0c00780].A smart diffraction grating immunosensor based on antigen-responsive hydrogel with enhanced analyte-induced volume changes is developed for highly selective and sensitive detection of human immunoglobulin G (H-IgG). The hydrogel grating contains poly(N-isopropylacrylamide) (PNIPAM) backbones with dual-cross-linking based on the dynamic complexation between pendent goat-anti-human IgG (GAH-IgG) and pendent H-IgG, and the covalent bonding by 4-arm-polyethylene glycol-acrylamide. Upon recognizing free H-IgG in the environment, the pendent GAH-IgG in the hydrogel can form new GAH-IgG/H-IgG complexes with free H-IgG because the binding constant of GAH-IgG to the free H-IgG is much larger than that of GAH-IgG to the pendent H-IgG and thus result in the decomplexation of GAH-IgG/H-IgG complexes with the pendent H-IgG as well as the swelling of hydrogel. The thermo-responsive PNIPAM backbones enable enhancement of H-IgG-responsive volume change of the proposed hydrogel grating via temperature regulation. Moreover, the cross-linker 4-arm-polyethylene glycol-acrylamide provides excellent transparency for the PNIPAM backbones during the volume change, which ensures output of diffracted optical signals with high intensity. With the elaborately designed molecular structures, the hydrogel grating allows highly selective and sensitive detection of [H-IgG] with a detection limit as low as 1.3 × 10–8 M. This work provides a simple and flexible strategy for developing diffraction grating immunosensors based on stimuli-responsive hydrogels for efficient detection of biomarkers