Complement C5a receptor gene 450 C/T polymorphism in renal transplant recipients: Association of the CT genotype with graft outcome

PubMedID: 25582053Complement-mediated humoral rejection has become the main focus of research in organ transplantation. The aim of this study was to investigate the possible association of the complement C5aR gene 450 C/T polymorphism in antibody-mediated renal allograft rejection. This polymorphism was investigated in 290 first deceased donor kidney graft recipients with well functioning grafts and no rejection treatment during the first transplant year (WFG), 265 recipients with graft failure within the first transplant year (F), and 187 healthy controls. Frequency of the 450 CT genotype was lower in the total population of 555 kidney recipients (4.7%) than in 187 healthy controls (8.6%), but the difference was not statistically significant (P=0.065). A significantly higher frequency of CT genotype was found in F patients (CT: 6.8%) when compared to WFG patients (CT: 2.8%, P=0.027). The CT genotype was also significantly lower in WFG patients than in healthy controls (P=0.009). Low frequency of the C5aR 450 CT genotype, which apparently is a feature of certain kidney diseases, appears to be associated with good graft outcome in kidney transplantation and might be helpful for identifying recipients who are at low risk for graft rejection. © 2015 John Wiley & Sons A/S.Deutsche Forschungsgemeinschaft: GZ:446TUR112/8/0

VEGF 936 C/T gene polymorphism in renal transplant recipients: Association of the T allele with good graft outcome

PubMedID: 17584582We investigated the possible impact of vascular endothelial growth factor (VEGF) 936 C/T gene polymorphism on kidney graft outcome. DNA samples of 290 first deceased donor kidney graft recipients with well-functioning grafts and no rejection treatment during the first transplant year (WFG), 265 recipients with graft failure within the first transplant year (F), and 187 healthy control subjects were tested using the polymerase chain reaction restriction fragment length polymorphism technique. Although VEGF 936 CT genotype and T allele carrier frequencies in 555 kidney graft recipients did not differ significantly from frequencies observed in healthy control subjects, significantly higher frequencies were found in WFG patients (CT: 19.0%, T: 20.7%) than in F patients (CT: 11.7%, p = 0.019; T: 12.8%, p = 0.017, respectively). The VEGF 936 CT genotype and T allele appear to be associated with good outcome in renal transplantation and, if confirmed, might be helpful for the pretransplant identification of recipients with low risk of graft rejection. © 2007 American Society for Histocompatibility and Immunogenetics.Universität Heidelberg Deutsche ForschungsgemeinschaftThe authors thank the Collaborative Transplant Study (CTS), Heidelberg, Germany, for financial support and DNA samples. This work was supported by the Scientific and Technological Research Council of Turkey (TUBITAK)” and a scholarship award of the Deutsche Forschungsgemeinschaft (DFG) to R.G., who performed this work at the Department of Transplantation Immunology, University of Heidelberg, Germany