Study on the Interaction of Pyrene and Humic Acid by Fluorometry

[中文文摘]建立了用荧光猝灭法研究芘与腐植酸间的相互作用的方法． 本方法的优点是不经分离即可测定荧光物的结合常数．[英文文摘]A fluorescence quenching method has been developed for determination association constant of pyrene with dissolved humic acids.The technique is based upon the observation that pyrene fluorescence in aqueous solution is quenched upon association with humic acid.The great advantage of this method is that the association constant of some kinds of fluorephors in a sample can be measured without separation

Determination of 1-Hydroxy Pyrene in Fish Bile by Synchronous Fluorescence Spectrometry

[中文文摘]建立了用同步荧光法测定鱼胆汁中的 1 羟基芘 (1 HP)的方法。该方法通过直接测定鱼胆汁的多环芳烃 (PAHs)的代谢产物之一 1 HP ,用于判断PAHs生物有效部分对海岸带养殖水环境的污染程度。以真鲷(Pargrosomusmajor,简称Pm) ,　鱼 (Nibeamiichthys ,简称Nm)为实验对象 ,现场实验结果令人满意。[英文文摘]A method for the determination of 1 hydroxy pyrene in fish bile by synchronous fluorescnece spectrometry has been established. The experimental results shown that 1 hydroxy pyrene, one of the most imporant polynuclear aromatic hydrocarbons (PAHs) metabolites in fish bile, can be determined directly by synchronous fluorescence spectrometry. Fish bile samples were obtained form Pagrosomus major and Nibea miichthys, which were sampled at three different sampling stations in Xiamen marine cultural area.The usefulness of 12hydroxy pyrene analytical method in fish bile as a biomonitoring tool for PAHs pollution is discussed.国家自然科学基金(29977016); 福建省自然科学基金 (D9910008)资助项目