Perinatal variation and covariation of oxidative status and telomere length in yellow-legged gull chicks.

The perinatal period is critical to survival and performance of many organisms. In birds, rapid postnatal growth and sudden exposure to aerial oxygen around hatching markedly affect the chick redox status, with potentially negative consequences on physiology mediated by oxidative stress. In addition, telomere length (TL) undergoes reduction during birds' early life, partly depending on oxidative status. However, relatively few studies have focused specifically on the changes in oxidative status and TL that occur immediately after hatching. In this study of the yellow-legged gull Larus michahellis, we found that chicks undergo a marked increase in plasma total antioxidant capacity and a marked decrease in the concentration of pro-oxidant molecules during the first days after hatching. In addition, TL in erythrocytes decreased by 1 standard deviation over the 4 days post-hatching. Body mass and tarsus length covaried with total antioxidant capacity and concentration of pro-oxidants in a complex way, that partly depended on sex and laying order, suggesting that oxidative status can affect growth. Moreover, TL positively covaried with the concentration of pro-oxidant molecules, possibly because retention of high concentrations of pro-oxidant molecules results from mechanisms of prevention of their negative effects, including reduction in TL. Thus, this study shows that chicks undergo marked variation in oxidative status, which predicts growth and subsequent TL, prompting for more studies of the perinatal changes in the critical post-hatching stages

Ecological factors affecting foraging behaviour during nestling rearing in a high-elevation species, the White-winged Snowfinch (Montifringilla nivalis)

During breeding, parents of avian species must increase their foraging efforts to collect food for their offspring, besides themselves. Foraging trips are thus a key aspect of the foraging ecology of central-place foragers when rearing their offspring. However, studies of the foraging ecology of high-elevation specialists inhabiting harsh environments are scarce. Here we report for the first time quantitative information on ecological determinants of foraging trips in the White-winged Snowfinch (Montifringilla nivalis), a high-elevation specialist threatened by climate warming. We focused on seasonal, meteorological, habitat and social factors affecting distance and duration of foraging trips performed during nestling rearing, recorded by visual observations in the Italian Alps. Based on 309 foraging trips from 35 pairs, we found that trips lasted 6.12 min and foraging areas were located at 175 m from the nest site on average. Trip duration was affected by snow cover (longer at intermediate cover), distance travelled and wind, while distance travelled was affected by snow cover (being higher at intermediate cover) and trip duration. Foraging individuals thus travelled farther and spent more time at areas characterized by intermediate snow cover, implying the presence of snow margins. It is likely that at such snow patches/margins snowfinches collected food for self-maintenance, besides that for their offspring, or collected more food items. Any reduction of snow cover during the breeding season, as expected under current climate warming, will severely alter foraging habitat suitability. Conserving suitable foraging habitats in the nest surroundings will be crucial to buffer such negative impacts

Annual egg productivity predicts female-biased mortality in avian species

Among avian species, the differential cost entailed by either sex in competition for mates has been regarded as the main evolutionary influence on sex differences in mortality rates. However, empirical evidence suggests that sex-biased adult mortality is mainly related to differential energy investment in gamete production, with a greater annual mass devoted to egg production leading to higher female mortality. We explicitly tested the generality of this pattern in a comparative framework. Annual egg production can be relatively large in some species (up to 200% of female body mass) and annual mortality is generally biased toward females. We showed that greater annual egg productivity resulted in higher mortality rates of females relative to males. Mating system was secondarily important, with species in which males were more involved in mating competition having more equal mortality rates between the sexes. However, both traits explained only a limited fraction of the interspecific variation in female-biased mortality. Other traits, such as sexual size dimorphism and parental care, had much weaker influences on female-biased mortality. Our results suggest that both annual mass devoted to gamete production by females and mating system contribute to the evolution of the fundamental life-history trade-off between reproduction and survival in avian taxa

Inter-individual differences in foraging tactics of a colonial raptor : consistency, weather effects, and fitness correlates

Background: Consistent inter-individual differences in behavioural phenotypes may entail differences in energy efficiency and expenditure, with different fitness payoffs. In colonial-breeding species, inter-individual differences in foraging behaviour may evolve to reduce resource use overlap among conspecifics exploiting shared foraging areas. Furthermore, individual differences in foraging behaviour may covary with individual characteristics, such as sex or physiological conditions. Methods: We investigated individual differences in foraging tactics of a colonial raptor, the lesser kestrel (Falco naumanni). We tracked foraging trips of breeding individuals using miniaturized biologgers. We classified behaviours from GPS data and identified tactics at the foraging trip level by cluster analysis. We then estimated energy expenditure associated to each tactic from tri-axial accelerometer data. Results: We obtained 489 foraging trips by 36 individuals. Two clusters of trips were identified, one (SF) characterized by more static foraging behaviour and the other (DF) by more dynamic foraging behaviour, with a higher proportion of flying activity and a higher energy expenditure compared to SF. Lesser kestrels showed consistent inter-individual differences in foraging tactics across weather condition gradients, favouring DF trips as solar radiation and crosswind intensity increased. DF trips were more frequent during the nestling-rearing than during the egg incubation stage. Nestlings whose tracked parent was more prone to perform DF trips experienced higher daily mass increase, irrespective of nestling feeding rates. Conclusions: Our study provided evidence that breeding lesser kestrels flexibly adopted different foraging tactics according to contingent weather landscapes, with birds showing consistent inter-individual differences in the tendency to adopt a given tactic. The positive correlation between the tendency to perform more energy-demanding DF trips and nestling growth suggests that individual differences in foraging behaviour may play a role in maintaining key life-history trade-offs between reproduction and self-maintenance

Winter roost occupancy and behaviour at evening departure of urban long‐eared owls

Roost occupancy and behaviour at evening departure were studied in long-eased owls (Asio otus) at a large winter roost in the southern suburbs of the city of Milan, northern Italy. The number of roosting owls was strongly correlated with decreasing daylength, while it was weakly negatively correlated with temperature. Hence changes in photoperiod can be considered among the proximate factors promoting the winter aggregation of long-eared owls. Behaviour at departure was influenced by cloud cover: birds departed earlier, and departures were at a higher altitude and less concentrated, with covered sky than with clear sky; the reasons for these behavioural differences remain unclear. Directions of departure showed that owls do not use the urban area for hunting, as no birds were observed flying towards the city, consistently with dietary data

Remote sensing reveals scale‐specific effects of forage crop mowing and landscape structure on a declining farmland bird

The effectiveness of agri-environment schemes (AESs), the largest conservation-related expenditure for farmland biodiversity conservation within the European Union, is often compromised by a limited spatial scale of implementation. We focused on multiannual forage crops, a surrogate habitat for grassland birds, to assess the scale-dependent effects of mowing timing and frequency on the local population size of an iconic species, the skylark (Alauda arvensis). While there is much evidence for a negative impact of in-field mowing activities on grassland birds, whether such effects occur also at broader spatial scales is largely unknown. We surveyed breeding skylarks in the Po Plain (northern Italy) to determine (1) the association between landscape composition/configuration and abundance and (2) how abundance is affected by forage crop mowing timing and frequency. We addressed both questions through scale optimisation, identifying the most influential spatial scales for each covariate. Forage crop mowing timing was assessed through a novel remote sensing algorithm based on high-resolution Sentinel-2 satellite images. We observed a strong scale dependence on the importance of different habitats in determining skylark abundance. Abundance increased with an increasing cover of forage crops locally (200 m) and of winter crops at a landscape scale (2600 m), suggesting that the species is favoured by heterogeneous agroecosystems. Locally (150–350 m), skylarks were more abundant when crops were aggregated, being negatively impacted by crop fragmentation caused by urbanization and by seminatural habitats. At the landscape scale (1150 m), the timing of mowing was consistent across years, with early-mown areas supporting fewer skylarks. This is probably because, over longer temporal scales, early-mown forage patches have limited or null productivity, eventually limiting local population size. Synthesis and applications. We provide a new perspective on the overarching influence of spatial scale in driving the abundance of a declining farmland bird species, supporting the urgency of designing landscape scale-effective AESs. This should be framed within the new EU Common Agricultural Policy reform and operated by farmer collectives, whereby management interventions should be monitored by state-of-the-art remote sensing techniques. These results suggest that implementing scale-optimized AESs could be crucial for effective farmland biodiversity conservatio

No evidence of avian malaria in two Mediterranean endemic seabirds

In birds, pathogens and diseases—such as avian malaria—can have severe detrimental effects on individual fitness. Pathogen prevalence can vary across species and may differ between populations living in different localities, but screening can aid in our understanding of a disease’s distribution and parasite-host interactions. Although seabirds generally exhibit low avian malaria infection patterns, blood parasites of several species and populations have never been investigated in detail. Using molecular techniques, we screened for blood parasites in two Mediterranean seabirds, the Scopoli’s Shearwater Calonectris diomedea and the Mediterranean Storm Petrel Hydrobates pelagicus melitensis. In addition, we searched for and sampled potential vector insects at each seabird colony. DNA was extracted from blood samples (or whole specimens for vector insect species), and polymerase chain reaction was performed to assess the presence of Plasmodium, Haemoproteus, and Leucocytozoon, the most frequent infective protozoan genera. Our results showed no evidence of haemosporidians, either in the sampled species or in the vector insects. The low prevalence of parasites in these species could reflect the absence or rarity of the vector for transmission. Thus, extreme care must be taken when releasing individuals into the wild to avoid introducing infection into new seabird populations

Eco-evolutionary drivers of avian migratory connectivity

Migratory connectivity, reflecting the extent by which migrants tend to maintain their reciprocal positions in seasonal ranges, can assist in the conservation and management of mobile species, yet relevant drivers remain unclear. Taking advantage of an exceptionally large (similar to 150,000 individuals, 83 species) and more-than-a-century-long dataset of bird ringing encounters, we investigated eco-evolutionary drivers of migratory connectivity in both short- and long-distance Afro-Palearctic migratory birds. Connectivity was strongly associated with geographical proxies of migration costs and was weakly influenced by biological traits and phylogeny, suggesting the evolutionary lability of migratory behaviour. The large intraspecific variability in avian migration strategies, through which most species geographically split into distinct migratory populations, explained why most of them were significantly connected. By unravelling key determinants of migratory connectivity, our study improves knowledge about the resilience of avian migrants to ecological perturbations, providing a critical tool to inform transboundary conservation and management strategies at the population level

Year-round variation in the isotopic niche of Scopoli's shearwater (Calonectris diomedea) breeding in contrasting sea regions of the Mediterranean Sea

Top marine predators are key components of marine food webs. Among them, long-distance migratory seabirds, which travel across different marine ecosystems over the year, may experience important year-round changes in terms of oceanographic conditions and availability of trophic resources. We tested whether this was the case in the Scopoli's shearwater (Calonectris diomedea), a trans-equatorial migrant and top predator, by sampling birds breeding in three environmentally different regions of the Mediterranean Sea. The analysis of positional data and stable isotopes (δ1³C and δ15N) of target feathers revealed that birds from the three regions were spatially segregated during the breeding period while they shared non-breeding areas in the Atlantic Ocean. Isotopic baseline levels of N and C (meso-zooplankton) were significantly different among marine regions during breeding. Such variation was reflected at the higher trophic levels of pelagic and demersal fish muscles as well as in shearwater feathers grown in the Mediterranean. δ15N- and δ13C-adjusted values of shearwaters were significantly different among populations suggesting that birds from different breeding areas relied on prey species from different trophic levels. Conversely, the non-breeding spatial and isotopic niches overlapped greatly among the three populations. Shearwater trophic niches during breeding were narrower and segregated compared to the non-breeding period, revealing a high plasticity in trophic resource use. Overall, this study highlights seasonal and region-specific use of trophic resources by Scopoli's shearwater, suggesting a broad trophic plasticity and possibly a high adaptability to environmental changes