Histone Mutants Separate R Loop Formation from Genome Instability Induction

R loops have positive physiological roles, but they can also be deleterious by causing genome instability, and the mechanisms for this are unknown. Here we identified yeast histone H3 and H4 mutations that facilitate R loops but do not cause instability. R loops containing single-stranded DNA (ssDNA), versus RNA-DNA hybrids alone, were demonstrated using ssDNA-specific human AID and bisulfite. Notably, they are similar size regardless of whether or not they induce genome instability. Contrary to mutants causing R loop-mediated instability, these histone mutants do not accumulate H3 serine-10 phosphate (H3S10-P). We propose a two-step mechanism in which, first, an altered chromatin facilitates R loops, and second, chromatin is modified, including H3S10-P, as a requisite for compromising genome integrity. Consistently, these histone mutations suppress the high H3S10 phosphorylation and genomic instability of hpr1 and sen1 mutants. Therefore, contrary to what was previously believed, R loops do not cause genome instability by themselves.European Research Council ERC2014 AdG669898Ministerio de Economía y Competitividad BFU2013-42918-P, BFU2016-75058-

Seeing topological edge and bulk currents in time-of-flight images

5 pags., 5 figs.Here we provide a general methodology to directly measure the topological currents emerging in the optical lattice implementation of the Haldane model. Alongside the edge currents supported by gapless edge states, transverse currents can emerge in the bulk of the system whenever the local potential is varied in space, even if it does not cause a phase transition. In optical lattice implementations the overall harmonic potential that traps the atoms provides the boundaries of the topological phase that supports the edge currents, as well as providing the potential gradient across the topological phase that gives rise to the bulk current. Both the edge and bulk currents are resilient to several experimental parameters such as trapping potential, temperature, and disorder. We propose to investigate the properties of these currents directly from time-of-flight images with both short-time and long-time expansions.This work was supported by the EPSRC Grant No. EP/R020612/1; Spanish Projects PGC2018-094792-B-I00 (MCIU/AEI/FEDER, EU), PGC2018-094180-B-I00 (MCIU /AEI/FEDER, EU), and FIS2015-63770-P (MINECO /FEDER, EU); CAM/FEDER Project No. S2018/TCS-4342 (QUITEMAD-CM), and CSIC Research Platform PTI-001