Changing trends of pyogenic microorganisms in a tertiary care hospital

Objective: To determine the predominant microorganisms responsible for pyogenic infections and prospective monitoring of the changing trends in their antibiotic susceptibility profile. Methodology: A cross sectional study was conducted at the Department of Microbiology, Rawal Institute of Health Sciences, Islamabad, from April 2015 to February 2016. Pus and pus swab samples were randomly collected from different body sites both from the males and females from any age group. Patients who were already taking antibiotics, those on steroids or suffering from chronic illnesses were excluded from the study. Inoculation of the samples on the respective culture media was done followed by Gram stain, biochemical reactions and antibiotic susceptibility testing carried out by Kirby Bauer disc diffusion method and its interpretation as per Clinical Laboratory and Standards Institute (CLSI) guidelines. Data was collected on preformed table and analysis was done by using SPSS version 21. Results: 127 pus samples were analyzed, out of which 103 yielded a positive culture. Staphylococcus aureus/Methicillin resistant Staphylococcus aureus (MRSA) was the commonest organism among the Gram positive organisms which showed maximum susceptibility to vancomycin (100%) and Linezolid (92%). Among the Gram negative organisms Escherichia coli was the commonest followed by Pseudomonas aeruginosa and other organisms. Conclusion:  The changing trends of pyogenic organisms have to be monitored prospectively to counter the global epidemic and to detect the constantly increasing burden of antibiotic resistance leading to it

Studies on the Generation of Biogas from Collagen Hydrolysate obtained from Chrome Shavings by Alkaline Hydrolysis: A Greener Disposal Method

Abstract The leather industry produces a significant amount of chromium bearing hazardous waste. Soli

Assessment of Genetic Variability for Morpho-physiological and Yield Traits in Bread Wheat (Triticum aestivum L.)

In order to estimate genetic variability parameters for seventeen traits of wheat, 28 F1s were obtained by crossing 8 parents in 8×8 diallel fashion, excluding reciprocals and evaluation trial was laid down in randomised complete block design at Pantnagar, Uttarakhand. Analysis of variance revealed that highly significant differences between genotypes exist for all seventeen characters. Eleven out of seventeen characters were observed to exhibit elevated values for both the PCV and GCV. The observed phenotypic coefficient of variation (PCV) values was determined to be greater than the genotypic coefficient of variation (GCV) values, indicating that the expression of traits is comparatively less influenced by environmental factors. A significant degree of heritability, along with a notable genetic advance, was observed for various traits, including flag leaf area, followed by grain yield, biological yield, canopy temperature depression (CTD), harvest index, tillers per plant, peduncle length, grains per spike, awn length, plant height, grain filling duration, spikelet per spike, spike length, and normalised difference vegetation index (NDVI) content. This study has thus identified significant genetic variability in wheat traits, highlighted traits with high heritability and genetic advance, and suggested that these traits could be targeted for improvement in wheat breeding programs

Changing trends of pyogenic microorganisms in a tertiary care hospital

Objective: To determine the predominant microorganisms responsible for pyogenic infections and prospective monitoring of the changing trends in their antibiotic susceptibility profile. Methodology: A cross sectional study was conducted at the Department of Microbiology, Rawal Institute of Health Sciences, Islamabad, from April 2015 to February 2016. Pus and pus swab samples were randomly collected from different body sites both from the males and females from any age group. Patients who were already taking antibiotics, those on steroids or suffering from chronic illnesses were excluded from the study. Inoculation of the samples on the respective culture media was done followed by Gram stain, biochemical reactions and antibiotic susceptibility testing carried out by Kirby Bauer disc diffusion method and its interpretation as per Clinical Laboratory and Standards Institute (CLSI) guidelines. Data was collected on preformed table and analysis was done by using SPSS version 21. Results: 127 pus samples were analyzed, out of which 103 yielded a positive culture. Staphylococcus aureus/Methicillin resistant Staphylococcus aureus (MRSA) was the commonest organism among the Gram positive organisms which showed maximum susceptibility to vancomycin (100%) and Linezolid (92%). Among the Gram negative organisms Escherichia coli was the commonest followed by Pseudomonas aeruginosa and other organisms. Conclusion:  The changing trends of pyogenic organisms have to be monitored prospectively to counter the global epidemic and to detect the constantly increasing burden of antibiotic resistance leading to it