2 research outputs found
CHANGES IN THE STRUCTURAL CHARACTERISTICS OF PLATELETS AND LEUKOCYTES IN PATIENTS WITH ARTERIAL HYPERTENSION UNDER THE INFLUENCE OF A HYDROGEN SULFIDE DONOR
The aim of the research. The influence of hydrogen sulphide donor on some structural characteristics of platelets and leukocytes in patients with arterial hypertension was studied.
Materials and methods. The survey involved patients (men) with hypertension aged 30β60 years, who were divided into 2 age groups: 30β40 years and 40β60 years. Control groups (healthy men without signs of hypertension) were randomized by age. Electron microscopic and morphometric research methods were used in the investigation.
Results. Electron microscopic and morphometric evaluation of the structure of these cells has shown that it undergoes significant changes depending on patientβs age. In the persons of the older age group, the cells contained a reduced number of dense Ξ΄-granules and increased β Ξ±-granules, which may indicate an impaired serotonin balance and increased susceptibility to thrombogenesis. The younger age group presented structural changes in platelets in a reversed order. Adding hydrogen sulphide donor at a dose of 400 mg per day (phytopreparation Full Spectrum Garlic β Swanson Health Products, USA) to the traditional therapy of arterial hypertension leads to positive changes in the ultrastructure of the studied cells aimed at restoring the balance of serotonin, reducing the tendency to thrombogenesis, increasing the energy capacity of the mitochondrial apparatus.
Conclusion. The obtained results indicate that detected effects significantly depend on the patientβs age (younger people respond more intensively) and the duration of the use of hydrogen sulphide donor (longer use is accompanied by more pronounced positive changes)
Π―ΠΠΠ―ΠΠ’Π‘Π― ΠΠ ΠΠ ΠΠΠΠΠ‘ΠΠΠ¦ΠΠ€ΠΠ§ΠΠΠ ΠΠΠ‘ΠΠ ΠΠ‘Π‘ΠΠ― ΠΠΠΠΠ-ΠΠΠΠΠΠΠΠ’ΠΠ (DJ1, PINK1) Π Π’ΠΠΠΠ―Π₯ ΠΠ ΠΠΠΠΠΠΠ ΠΠ Π ΠΠΠ‘ΠΠΠ ΠΠΠΠΠ’ΠΠΠ¬ΠΠΠ ΠΠΠ ΠΠΠΠ‘ΠΠΠΠΠΠ Π ΠΠΠ ΠΠΠ’ΠΠΠΠΠΠ’ΠΠ§ΠΠ‘ΠΠΠ Π’ΠΠ ΠΠΠΠ?
It have been studied the changes in the structural and functional state of mitochondria and expression of PINK1 and DJ1 genes in brain tissue - medulla oblongata and striatum and lung and heart tissue in experimental parkinsonism and its pathogenetic treatment with the help of a broad-spectrum antihypoxant Kapikor. It was shown that undrt experimental parkinsonism, in addition to damage to the ultrastructure of the mitochondrial apparatus in cells of body tissues, there are significant changes in mRNA expression of DJ1 and PINK1 genes, which are associated with the formation of mitochondrial dysfunction. They have a multidirectional character in the tissues of the brain - decrease, and in the tissues of the heart and lungs - increase. The degree of such changes in expression is organ-specific and more pronounced in the tissues of the visceral organs than in the tissues of the brain. Also, it was shown that the use of broad-spectrum antioxidant, which contains mildenium dehydrate and gamma-butyrobetaine dihydrate, there are significant changes in the expression of mRNA genes DJ1 and PINK1, which are also organ-specific - the expression of mRNA of all DJ1 genes increased in to a greater extent, the expression of PINK1 gene mRNA decreased sharply in brain tissues, and also increased sharply in lung and heart tissues. The data obtained indicate a complex and ambiguous relationship between the level of expression of the studied candidate genes involved in the formation of experimental parkinsonism, and the severity of mitochondrial dysfunction, which is one of the pathogenetic causes of parkinsonism.ΠΠ·ΡΡΠ΅Π½Ρ ΠΈΠ·ΠΌΠ΅Π½Π΅Π½ΠΈΡ ΡΡΡΡΠΊΡΡΡΠ½ΠΎ-ΡΡΠ½ΠΊΡΠΈΠΎΠ½Π°Π»ΡΠ½ΠΎΠ³ΠΎ ΡΠΎΡΡΠΎΡΠ½ΠΈΡ ΠΌΠΈΡΠΎΡ
ΠΎΠ½Π΄ΡΠΈΠΉ ΠΈ ΡΠΊΡΠΏΡΠ΅ΡΡΠΈΠΈ Π³Π΅Π½ΠΎΠ² PINK1 ΠΈ DJ1 Π² ΡΠΊΠ°Π½ΠΈ Π³ΠΎΠ»ΠΎΠ²Π½ΠΎΠ³ΠΎ ΠΌΠΎΠ·Π³Π° - ΠΏΡΠΎΠ΄ΠΎΠ»Π³ΠΎΠ²Π°ΡΠΎΠΌ ΠΌΠΎΠ·Π³Π΅ ΠΈ ΡΡΡΠΈΠ°ΡΡΠΌΠ΅, Π° ΡΠ°ΠΊΠΆΠ΅ Π² ΡΠΊΠ°Π½ΠΈ Π»Π΅Π³ΠΊΠΈΡ
ΠΈ ΡΠ΅ΡΠ΄ΡΠ° ΠΏΡΠΈ ΡΠΊΡΠΏΠ΅ΡΠΈΠΌΠ΅Π½ΡΠ°Π»ΡΠ½ΠΎΠΌ ΠΏΠ°ΡΠΊΠΈΠ½ΡΠΎΠ½ΠΈΠ·ΠΌΠ΅ ΠΈ Π΅Π³ΠΎ ΠΏΠ°ΡΠΎΠ³Π΅Π½Π΅ΡΠΈΡΠ΅ΡΠΊΠΎΠΌ Π»Π΅ΡΠ΅Π½ΠΈΠΈ Ρ ΠΏΠΎΠΌΠΎΡΡΡ Π°Π½ΡΠΈΠ³ΠΈΠΏΠΎΠΊΡΠ°Π½ΡΠ° ΡΠΈΡΠΎΠΊΠΎΠ³ΠΎ ΡΠΏΠ΅ΠΊΡΡΠ° Π΄Π΅ΠΉΡΡΠ²ΠΈΡ ΠΠ°ΠΏΠΈΠΊΠΎΡΠ°. ΠΡΠ»ΠΎ ΠΏΠΎΠΊΠ°Π·Π°Π½ΠΎ, ΡΡΠΎ ΠΏΡΠΈ ΡΠΊΡΠΏΠ΅ΡΠΈΠΌΠ΅Π½ΡΠ°Π»ΡΠ½ΠΎΠΌ ΠΏΠ°ΡΠΊΠΈΠ½ΡΠΎΠ½ΠΈΠ·ΠΌΠ΅, ΠΏΠΎΠΌΠΈΠΌΠΎ ΠΏΠΎΠ²ΡΠ΅ΠΆΠ΄Π΅Π½ΠΈΡ ΡΠ»ΡΡΡΠ°ΡΡΡΡΠΊΡΡΡΡ ΠΌΠΈΡΠΎΡ
ΠΎΠ½Π΄ΡΠΈΠ°Π»ΡΠ½ΠΎΠ³ΠΎ Π°ΠΏΠΏΠ°ΡΠ°ΡΠ° Π² ΠΊΠ»Π΅ΡΠΊΠ°Ρ
ΡΠΊΠ°Π½Π΅ΠΉ ΠΎΡΠ³Π°Π½ΠΈΠ·ΠΌΠ°, ΠΏΡΠΎΠΈΡΡ
ΠΎΠ΄ΡΡ Π·Π½Π°ΡΠΈΡΠ΅Π»ΡΠ½ΡΠ΅ ΠΈΠ·ΠΌΠ΅Π½Π΅Π½ΠΈΡ Π² ΡΠΊΡΠΏΡΠ΅ΡΡΠΈΠΈ ΠΌΠ ΠΠ Π³Π΅Π½ΠΎΠ² DJ1 ΠΈ PINK1, ΠΊΠΎΡΠΎΡΡΠ΅ ΡΠ²ΡΠ·Π°Π½Ρ Ρ ΡΠΎΡΠΌΠΈΡΠΎΠ²Π°Π½ΠΈΠ΅ΠΌ ΠΌΠΈΡΠΎΡ
ΠΎΠ½Π΄ΡΠΈΠ°Π»ΡΠ½ΠΎΠΉ Π΄ΠΈΡΡΡΠ½ΠΊΡΠΈΠΈ. Π ΡΠΊΠ°Π½ΡΡ
Π³ΠΎΠ»ΠΎΠ²Π½ΠΎΠ³ΠΎ ΠΌΠΎΠ·Π³Π° ΠΎΠ½Π° ΠΈΠΌΠ΅Π΅Ρ ΠΎΠ΄Π½ΠΎΠ½Π°ΠΏΡΠ°Π²Π»Π΅Π½Π½ΡΠΉ Ρ
Π°ΡΠ°ΠΊΡΠ΅Ρ - ΡΠ½ΠΈΠΆΠ°Π΅ΡΡΡ, Π° Π² ΡΠΊΠ°Π½ΡΡ
ΡΠ΅ΡΠ΄ΡΠ° ΠΈ Π»Π΅Π³ΠΊΠΈΡ
- ΡΠ²Π΅Π»ΠΈΡΠΈΠ²Π°Π΅ΡΡΡ. Π‘ΡΠ΅ΠΏΠ΅Π½Ρ ΡΠ°ΠΊΠΈΡ
ΠΈΠ·ΠΌΠ΅Π½Π΅Π½ΠΈΠΉ ΡΠΊΡΠΏΡΠ΅ΡΡΠΈΠΈ ΠΎΡΠ³Π°Π½ΠΎΡΠΏΠ΅ΡΠΈΡΠΈΡΠ½Π° ΠΈ Π±ΠΎΠ»Π΅Π΅ Π²ΡΡΠ°ΠΆΠ΅Π½Π° Π² ΡΠΊΠ°Π½ΡΡ
Π²ΠΈΡΡΠ΅ΡΠ°Π»ΡΠ½ΡΡ
ΠΎΡΠ³Π°Π½ΠΎΠ², ΡΠ΅ΠΌ Π² ΡΠΊΠ°Π½ΡΡ
ΠΌΠΎΠ·Π³Π°. Π’Π°ΠΊΠΆΠ΅ Π±ΡΠ»ΠΎ ΠΏΠΎΠΊΠ°Π·Π°Π½ΠΎ, ΡΡΠΎ ΠΏΡΠΈ ΠΈΡΠΏΠΎΠ»ΡΠ·ΠΎΠ²Π°Π½ΠΈΠΈ Π°Π½ΡΠΈΠΎΠΊΡΠΈΠ΄Π°Π½ΡΠ° ΡΠΈΡΠΎΠΊΠΎΠ³ΠΎ ΡΠΏΠ΅ΠΊΡΡΠ° Π΄Π΅ΠΉΡΡΠ²ΠΈΡ, ΠΊΠΎΡΠΎΡΡΠΉ ΡΠΎΠ΄Π΅ΡΠΆΠΈΡ ΠΌΠΈΠ»ΡΠ΄ΠΎΠ½ΠΈΡ Π΄ΠΈΠ³ΠΈΠ΄ΡΠ°Ρ ΠΈ Π΄ΠΈΠ³ΠΈΠ΄ΡΠ°Ρ Π³Π°ΠΌΠΌΠ°-Π±ΡΡΠΈΡΠΎΠ±Π΅ΡΠ°ΠΈΠ½Π°, Π½Π°Π±Π»ΡΠ΄Π°ΡΡΡΡ Π·Π½Π°ΡΠΈΡΠ΅Π»ΡΠ½ΡΠ΅ ΠΈΠ·ΠΌΠ΅Π½Π΅Π½ΠΈΡ Π² ΡΠΊΡΠΏΡΠ΅ΡΡΠΈΠΈ Π³Π΅Π½ΠΎΠ² ΠΌΠ ΠΠ DJ1 ΠΈ PINK1, ΠΊΠΎΡΠΎΡΡΠ΅ ΡΠ°ΠΊΠΆΠ΅ ΡΠ²Π»ΡΡΡΡΡ ΠΎΡΠ³Π°Π½ΠΎΡΠΏΠ΅ΡΠΈΡΠΈΡΠ½ΡΠΌΠΈ - ΡΠΊΡΠΏΡΠ΅ΡΡΠΈΡ ΠΌΠ ΠΠ Π³Π΅Π½Π° DJ1 ΡΠ²Π΅Π»ΠΈΡΠΈΠ»ΠΈΡΡ Π² Π±ΠΎΠ»ΡΡΠ΅ΠΉ ΡΡΠ΅ΠΏΠ΅Π½ΠΈ, ΡΠΊΡΠΏΡΠ΅ΡΡΠΈΡ ΠΌΠ ΠΠ Π³Π΅Π½Π° PINK1 ΡΠ΅Π·ΠΊΠΎ ΡΠ½ΠΈΠ·ΠΈΠ»Π°ΡΡ Π² ΡΠΊΠ°Π½ΡΡ
ΠΌΠΎΠ·Π³Π°, Π° ΡΠ°ΠΊΠΆΠ΅ ΡΠ΅Π·ΠΊΠΎ ΡΠ²Π΅Π»ΠΈΡΠΈΠ»Π°ΡΡ Π² ΡΠΊΠ°Π½ΡΡ
Π»Π΅Π³ΠΊΠΈΡ
ΠΈ ΡΠ΅ΡΠ΄ΡΠ°. ΠΠΎΠ»ΡΡΠ΅Π½Π½ΡΠ΅ Π΄Π°Π½Π½ΡΠ΅ ΡΠΊΠ°Π·ΡΠ²Π°ΡΡ Π½Π° ΡΠ»ΠΎΠΆΠ½ΡΡ ΠΈ Π½Π΅ΠΎΠ΄Π½ΠΎΠ·Π½Π°ΡΠ½ΡΡ Π²Π·Π°ΠΈΠΌΠΎΡΠ²ΡΠ·Ρ ΠΌΠ΅ΠΆΠ΄Ρ ΡΡΠΎΠ²Π½Π΅ΠΌ ΡΠΊΡΠΏΡΠ΅ΡΡΠΈΠΈ ΠΈΠ·ΡΡΠ°Π΅ΠΌΡΡ
Π³Π΅Π½ΠΎΠ²-ΠΊΠ°Π½Π΄ΠΈΠ΄Π°ΡΠΎΠ², ΡΡΠ°ΡΡΠ²ΡΡΡΠΈΡ
Π² ΡΠΎΡΠΌΠΈΡΠΎΠ²Π°Π½ΠΈΠΈ ΡΠΊΡΠΏΠ΅ΡΠΈΠΌΠ΅Π½ΡΠ°Π»ΡΠ½ΠΎΠ³ΠΎ ΠΏΠ°ΡΠΊΠΈΠ½ΡΠΎΠ½ΠΈΠ·ΠΌΠ°, ΠΈ ΡΡΠΆΠ΅ΡΡΡΡ ΠΌΠΈΡΠΎΡ
ΠΎΠ½Π΄ΡΠΈΠ°Π»ΡΠ½ΠΎΠΉ Π΄ΠΈΡΡΡΠ½ΠΊΡΠΈΠΈ, ΠΊΠΎΡΠΎΡΠ°Ρ ΡΠ²Π»ΡΠ΅ΡΡΡ ΠΎΠ΄Π½ΠΎΠΉ ΠΈΠ· ΠΏΠ°ΡΠΎΠ³Π΅Π½Π΅ΡΠΈΡΠ΅ΡΠΊΠΈΡ
ΠΏΡΠΈΡΠΈΠ½ ΠΏΠ°ΡΠΊΠΈΠ½ΡΠΎΠ½ΠΈΠ·ΠΌΠ°