A Phase-Field Model of Spiral Dendritic Growth

Domains of condensed-phase monolayers of chiral molecules exhibit a variety of interesting nonequilibrium structures when formed via pressurization. To model these domain patterns, we add a complex field describing the tilt degree of freedom to an (anisotropic) complex-phase-field solidification model. The resulting formalism allows for the inclusion of (in general, non-reflection symmetric) interactions between the tilt, the solid-liquid interface, and the bond orientation. Simulations demonstrate the ability of the model to exhibit spiral dendritic growth.Comment: text plus Four postscript figure file

Whole-genome resequencing of Escherichia coli K-12 MG1655 undergoing short-term laboratory evolution in lactate minimal media reveals flexible selection of adaptive mutations

Background Short-term laboratory evolution of bacteria followed by genomic sequencing provides insight into the mechanism of adaptive evolution, such as the number of mutations needed for adaptation, genotype-phenotype relationships, and the reproducibility of adaptive outcomes. Results In the present study, we describe the genome sequencing of 11 endpoints of Escherichia coli that underwent 60-day laboratory adaptive evolution under growth rate selection pressure in lactate minimal media. Two to eight mutations were identified per endpoint. Generally, each endpoint acquired mutations to different genes. The most notable exception was an 82 base-pair deletion in the rph-pyrE operon that appeared in 7 of the 11 adapted strains. This mutation conferred an approximately 15% increase to the growth rate when experimentally introduced to the wild-type background and resulted in an approximately 30% increase to growth rate when introduced to a background already harboring two adaptive mutations. Additionally, most endpoints had a mutation in a regulatory gene (crp or relA, for example) or the RNA polymerase. Conclusions The 82 base-pair deletion found in the rph-pyrE operon of many endpoints may function to relieve a pyrimidine biosynthesis defect present in MG1655. In contrast, a variety of regulators acquire mutations in the different endpoints, suggesting flexibility in overcoming regulatory challenges in the adaptation

Chemically modified electrodes. Radiofrequency plasma polymerization of vinylferrocene on glassy carbon and platinum electrodes

Vinylferrocene can be polymerized in a radiofrequency Ar plasma to deposit in thin, adherent films on qlassy carbon and Pt electrodes. In acetonitrile solvent containinig tetraethylammonium perchlorate or tetrafluoroborate electrolyte, these films exhibit electrochemical reactivity qualitatively expectable for polyvinylferrocene films although analytical information on film composition shows some plasma damage and some oxygen incorporation has taken place. The relationship of observed electrochemistry to plasma reaction time and geometry is described, Quantities of electroactive ferrocene in the prepared films ranqe from 0.5 - 300 x 10-10 mole/cm.2 . The thinner films exhibit non-limiting transport of electrochemical charge, while a thick film gives a diffusion controlled response

Improving the operating efficiency of the more electric aircraft concept through optimised flight procedures

The increasing awareness of the environmental risks and costs due to the growing demand in aviation has prompted both academic and industrial research into short-term and long-term technologies which could help address the challenges. Among these, the more electric aircraft has been identified as a key design concept which would make aircraft more environmentally friendly and cost effective in the long run. Moreover, the notion of free-flight and optimised trajectories has been identified as a key operational concept which would help curb the environmental effects of aircraft as well as reduce overall costs. The research in this paper presents a methodology in which these two concepts can be coupled to study the benefits of more electric aircraft (MEA) flying optimised trajectories. A wide range of issues from aircraft performance, engine performance, airframe systems operation, power off-take penalties, emission modelling, optimisation algorithms and optimisation frameworks has been addressed throughout the study. The case study is based on a popular short haul flight between London Heathrow and Amsterdam Schiphol. The culmination of the study establishes the advantage of the MEA over conventional aircraft and also addresses the enhanced approach to the classical aircraft trajectory optimisation problem. The study shows that the operation procedures to achieve a minimum fuel burn are significantly different for a conventional aircraft and MEA. Trajectory optimisation reduced the fuel burn by 17.4% for the conventional aircraft and 12.2% for the more electric compared to the respective baseline cases. Within the constraints of the study, the minimum fuel burn trajectory for the MEA consumed 9.9% less fuel than the minimum fuel burn trajectory for the conventional aircraft

The Measurement and Prediction of Gaseous Hydrocarbon Fuel AutoIgnition Delay Time at Realistic Gas Turbine Operating Conditions,” ASME Paper

ABSTRACT Auto-ignition delay time measurements have been attempted for a variety of gaseous fuels on a flow rig at gas turbine relevant operating conditions. The residence time of the flow rig test section was approximately 175 ms. A chemical kinetic model has been used in Senkin, one of the applications within the Chemkin package, to predict the auto-ignition delay time measured in the experiment. The model assumes that chemistry is the limiting factor in the prediction and makes no account of the fluid dynamic properties of the experiment. Auto-ignition delay time events were successfully recorded for ethylene at approximately 16 bar, 850K and at equivalence ratios between 2.6 and 3.3. Methane, natural gas and ethylene (0.5 < φ < 2.5) failed to auto-ignite within the test section. Model predictions were found to agree with the ethylene measurements, although improved qualification of the experimental boundary conditions is required in order to better understand the dependence of auto-ignition delay on the physical characteristics of the flow rig. The chemical kinetic model used in this study was compared with existing 'low temperature' measurements and correlations for methane and natural gas and was found to be in good agreement

Recent radio talks

Recent Rural Radio Talks Dairy Hygiene is Important; - D.C. Mickle Farm Water Supplies; - K. Needham Handling the Apple Crop; - K. Whitely Renovation of Irrigated Pastures; - Dairying division More About Lupinosis; - H. W. Bennetts Progress in Doublegee Control; - Norman Halse Changing Practices in Tobacco Growing in W.A.; - G. A. Pearce Progress in doublegee control; - G.A. Pearce Some Uses of Radio Isotopes in Agriculture; - E. N. Fitzpatrick Guildford Grass—Sign of a Run-Down Pasture; - R. A. Bettenay Silage and when to feed it; - R. Bettenay Ants in the Apiary; - R. S. Coleman Bacterial Canker of Stone Fruits; - Olga M. Goss Poison Plants in the Home Garden; - R. D. Royce The Cabbage White Butterfly; - C. F. H. Jenkins Summer Treatments for San Jose Scale; - C. F. H. Jenkins Preparations for Fodder Conservation; - H.G. Cariss Sudan Grass Survived Dry Summer; - R.A. Bettenay Root Maggot Flies; - J. A. Button Fallowing for Cropping; - A.S. Wild Feeding for Milk Quality; - L. C. Snook The Rabbit— Friend or Foe. A. R. Tomlinso

Large introns in relation to alternative splicing and gene evolution: a case study of Drosophila bruno-3

Background: Alternative splicing (AS) of maturing mRNA can generate structurally and functionally distinct transcripts from the same gene. Recent bioinformatic analyses of available genome databases inferred a positive correlation between intron length and AS. To study the interplay between intron length and AS empirically and in more detail, we analyzed the diversity of alternatively spliced transcripts (ASTs) in the Drosophila RNA-binding Bruno-3 (Bru-3) gene. This gene was known to encode thirteen exons separated by introns of diverse sizes, ranging from 71 to 41,973 nucleotides in D. melanogaster. Although Bru-3's structure is expected to be conducive to AS, only two ASTs of this gene were previously described. Results: Cloning of RT-PCR products of the entire ORF from four species representing three diverged Drosophila lineages provided an evolutionary perspective, high sensitivity, and long-range contiguity of splice choices currently unattainable by high-throughput methods. Consequently, we identified three new exons, a new exon fragment and thirty-three previously unknown ASTs of Bru-3. All exon-skipping events in the gene were mapped to the exons surrounded by introns of at least 800 nucleotides, whereas exons split by introns of less than 250 nucleotides were always spliced contiguously in mRNA. Cases of exon loss and creation during Bru-3 evolution in Drosophila were also localized within large introns. Notably, we identified a true de novo exon gain: exon 8 was created along the lineage of the obscura group from intronic sequence between cryptic splice sites conserved among all Drosophila species surveyed. Exon 8 was included in mature mRNA by the species representing all the major branches of the obscura group. To our knowledge, the origin of exon 8 is the first documented case of exonization of intronic sequence outside vertebrates. Conclusion: We found that large introns can promote AS via exon-skipping and exon turnover during evolution likely due to frequent errors in their removal from maturing mRNA. Large introns could be a reservoir of genetic diversity, because they have a greater number of mutable sites than short introns. Taken together, gene structure can constrain and/or promote gene evolution

Comparing nuclear power trajectories in Germany and the UK: from ‘regimes' to ‘democracies’ in sociotechnical transitions and Discontinuities

This paper focuses on arguably the single most striking contrast in contemporary major energy politics in Europe (and even the developed world as a whole): the starkly differing civil nuclear policies of Germany and the UK. Germany is seeking entirely to phase out nuclear power by 2022. Yet the UK advocates a ‘nuclear renaissance’, promoting the most ambitious new nuclear construction programme in Western Europe.Here,this paper poses a simple yet quite fundamental question: what are the particular divergent conditions most strongly implicated in the contrasting developments in these two countries. With nuclear playing such an iconic role in historical discussions over technological continuity and transformation, answering this may assist in wider understandings of sociotechnical incumbency and discontinuity in the burgeoning field of‘sustainability transitions’. To this end, an ‘abductive’ approach is taken: deploying nine potentially relevant criteria for understanding the different directions pursued in Germany and the UK. Together constituted by 30 parameters spanning literatures related to socio-technical regimes in general as well as nuclear technology in particular, the criteria are divided into those that are ‘internal’ and ‘external’ to the ‘focal regime configuration’ of nuclear power and associated ‘challenger technologies’ like renewables. It is ‘internal’ criteria that are emphasised in conventional sociotechnical regime theory, with ‘external’ criteria relatively less well explored. Asking under each criterion whether attempted discontinuation of nuclear power would be more likely in Germany or the UK, a clear picture emerges. ‘Internal’ criteria suggest attempted nuclear discontinuation should be more likely in the UK than in Germany– the reverse of what is occurring. ‘External’ criteria are more aligned with observed dynamics –especially those relating to military nuclear commitments and broader ‘qualities of democracy’. Despite many differences of framing concerning exactly what constitutes ‘democracy’, a rich political science literature on this point is unanimous in characterising Germany more positively than the UK. Although based only on a single case,a potentially important question is nonetheless raised as to whether sociotechnical regime theory might usefully give greater attention to the general importance of various aspects of democracy in constituting conditions for significant technological discontinuities and transformations. If so, the policy implications are significant. A number of important areas are identified for future research, including the roles of diverse understandings and specific aspects of democracy and the particular relevance of military nuclear commitments– whose under-discussion in civil nuclear policy literatures raises its own questions of democratic accountability

Analyzing ChIP-chip Data Using Bioconductor

Analyzing ChIP-chip Data Using Bioconducto