Low-Cost Motility Tracking System (LOCOMOTIS) for time-lapse microscopy applications and cell visualisation

This article has been made available through the Brunel Open Access Publishing Fund.Direct visualisation of cells for the purpose of studying their motility has typically required expensive microscopy equipment. However, recent advances in digital sensors mean that it is now possible to image cells for a fraction of the price of a standard microscope. Along with low-cost imaging there has also been a large increase in the availability of high quality, open-source analysis programs. In this study we describe the development and performance of an expandable cell motility system employing inexpensive, commercially available digital USB microscopes to image various cell types using time-lapse and perform tracking assays in proof-of-concept experiments. With this system we were able to measure and record three separate assays simultaneously on one personal computer using identical microscopes, and obtained tracking results comparable in quality to those from other studies that used standard, more expensive, equipment. The microscopes used in our system were capable of a maximum magnification of 413.6x. Although resolution was lower than that of a standard inverted microscope we found this difference to be indistinguishable at the magnification chosen for cell tracking experiments (206.8x). In preliminary cell culture experiments using our system, velocities (mean mm/min ± SE) of 0.81±0.01 (Biomphalaria glabrata hemocytes on uncoated plates), 1.17±0.004 (MDA-MB-231 breast cancer cells), 1.24±0.006 (SC5 mouse Sertoli cells) and 2.21±0.01 (B. glabrata hemocytes on Poly-L-Lysine coated plates), were measured and are consistent with previous reports. We believe that this system, coupled with open-source analysis software, demonstrates that higher throughput time-lapse imaging of cells for the purpose of studying motility can be an affordable option for all researchers. © 2014 Lynch et al

Model Uncertainty and Liquidity

Extreme market outcomes are often followed by a lack of liquidity and a lack of trade. This market collapse seems particularly acute for markets where traders rely heavily on a specific empirical model such as in derivative markets. Asset pricing and trading, in these cases, are intrinsically model dependent. Moreover, the observed behavior of traders and institutions that places a large emphasis on 'worst-case scenarios'' through the use of 'stress testing'' and 'value-at-risk'' seems different than Savage rationality (expected utility) would suggest. In this paper we capture model-uncertainty explicitly using an Epstein-Wang (1994) uncertainty-averse utility function with an ambiguous underlying asset-returns distribution. To explore the connection of uncertainty with liquidity, we specify a simple market where a monopolist financial intermediary makes a market for a propriety derivative security. The market-maker chooses bid and ask prices for the derivative, then, conditional on trade in this market, chooses an optimal portfolio and consumption. We explore how uncertainty can increase the bid-ask spread and, hence, reduces liquidity. In addition, 'hedge portfolios'' for the market-maker, an important component to understanding spreads, can look very different from those implied by a model without Knightian uncertainty. Our infinite-horizon example produces short, dramatic decreases in liquidity even though the underlying environment is stationary.

Generalized Disappointment Aversion and Asset Prices

We provide an axiomatic model of preferences over atemporal risks that generalizes Gul (1991) A Theory of Disappointment Aversion' by allowing risk aversion to be first order' at locations in the state space that do not correspond to certainty. Since the lotteries being valued by an agent in an asset-pricing context are not typically local to certainty, our generalization, when embedded in a dynamic recursive utility model, has important quantitative implications for financial markets. We show that the state-price process, or asset-pricing kernel, in a Lucas-tree economy in which the representative agent has generalized disappointment aversion preferences is consistent with the pricing kernel that resolves the equity-premium puzzle. We also demonstrate that a small amount of conditional heteroskedasticity in the endowment-growth process is necessary to generate these favorable results. In addition, we show that risk aversion in our model can be both state-dependent and counter-cyclical, which empirical research has demonstrated is necessary for explaining observed asset-pricing behavior.

Endocrine disrupting effects on the nesting behaviour of male three-spined stickleback Gasterosteus aculeatus L

The analysis of patterns of temporal variability in the nesting behaviour of male threespined stickleback (Gasterosteus aculeatus) exposed to the synthetic oestrogen, 17β-ethinylestradiol, revealed immediate, but transient, treatment-related effects. Gluing frequency and time spent near nest were significantly reduced in exposed fish at the beginning of the experiment. The expression of these behaviours subsequently recovered and there was no effect of treatment on nest building success. The potential causes and implications of these findings are discussed

Aquaculture diversification in South America: general views and facts and case studies of the Republic of Chile and the Federative Republic of Brazil.

Aquaculture and aquaculture diversification in South America: Facts and figures. Diversification of aquaculture in South America: a few figures and concepts. Aquaculture and aquaculture diversification in the Republic of Chile: Current situation, production models and strategies. The aquaculture diversification process in the Republic of Chile: recent history and current status. Diversification and the role of government and private industry. Technology and expertise, markets, institutional facilities and governance as drivers and/or constraints to aquaculture diversification in the Republic of Chile. The future of aquaculture diversification in the Republic of Chile. Aquaculture and aquaculture diversification in the Federative Republic of Brazil: Current situation and main species farmed. Recent history and current status of aquaculture diversification: Main drivers, constraints and species. The role of government, private industry and international organizations in aquaculture diversification. Technology and expertise, markets and institutional facilities as drivers and constraints. The future of aquaculture diversification: Main concerns, opportunities, restrictions, main species to consider.bitstream/item/164749/1/CNPASA-2017-fao.pd

Model Uncertainty and Liquidity

We investigate the dynamic portfolio problem of a market-maker for a derivative security whose preferences exhibit uncertainty aversion (Knightian uncertainty). The Choquet-expected utility implied by such preference is used to capture the feature that the trader is uncertain about which model should be used. The prices that emerge from the model are similar to standard models and have the feature that as uncertainty is removed, the derivative prices converge to standard prices. However, the optimal changes in the agent's portfolio that results from the option position are quite different than the standard hedge position. It is this feature that links uncertainty with market liquidity.

"Recursive Preferences,"

We summarize the class of recursive preferences. These preferences fit naturally with recursive solution methods and hold the promise of generating new insights into familiar problems. Portfolio choice is used as an example

Competition between Intramolecular and Intermolecular Interactions in an Amyloid-Forming Protein

Despite much progress in understanding the folding and the aggregation processes of proteins, the rules defining their interplay have yet to be fully defined. This problem is of particular importance since many diseases are initiated by protein unfolding and hence the propensity to aggregate competes with intramolecular collapse and other folding events. Here, we describe the roles of intramolecular and intermolecular interactions in defining the length of the lag time and the apparent rate of elongation of the 100-residue protein human β2-microglobulin at pH 2.5, commencing from an acid-denatured state that lacks persistent structure but contains significant non-random hydrophobic interactions. Using a combination of site-directed mutagenesis, quantitative kinetic analysis and computational methods, we show that only a single region of about 10 residues in length, determines the rate of fibril formation, despite the fact that other regions exhibit a significant intrinsic propensity for aggregation. We rationalise these results by analysing the effect of incorporating the conformational properties of acid-unfolded β2-microglobulin and its variants at pH 2.5 as measured by NMR spectroscopy into the Zyggregator aggregation prediction algorithm. These results demonstrate that residual structure in the precursor state modulates the intrinsic propensity of the polypeptide chain to aggregate and that the algorithm developed here allows the key regions for aggregation to be more clearly identified and the rates of their self-association to be predicted. Given the common propensity of unfolded chains to form non-random intramolecular interactions as monomers and to self-assemble subsequently into amyloid fibrils, the approach developed should find widespread utility for the prediction of regions important in amyloid formation and their rates of self-assembly. © 2009 Elsevier Ltd. All rights reserved

Automatic Detection of Expanding HI Shells Using Artificial Neural Networks

The identification of expanding HI shells is difficult because of their variable morphological characteristics. The detection of HI bubbles on a global scale therefore never has been attempted. In this paper, an automatic detector for expanding HI shells is presented. The detection is based on the more stable dynamical characteristics of expanding shells and is performed in two stages. The first one is the recognition of the dynamical signature of an expanding bubble in the velocity spectra, based on the classification of an artificial neural network. The pixels associated with these recognized spectra are identified on each velocity channel. The second stage consists in looking for concentrations of those pixels that were firstly pointed out, and to decide if they are potential detections by morphological and 21-cm emission variation considerations. Two test bubbles are correctly detected and a potentially new case of shell that is visually very convincing is discovered. About 0.6% of the surveyed pixels are identified as part of a bubble. These may be false detections, but still constitute regions of space with high probability of finding an expanding shell. The subsequent search field is thus significantly reduced. We intend to conduct in the near future a large scale HI shells detection over the Perseus Arm using our detector.Comment: 39 pages, 11 figures, accepted by PAS