The analysis of patterns of temporal variability in the nesting behaviour of male threespined stickleback (Gasterosteus aculeatus) exposed to the synthetic oestrogen, 17β-ethinylestradiol, revealed immediate, but transient, treatment-related effects. Gluing frequency and time spent near nest were significantly reduced in exposed fish at the beginning of the experiment. The expression of these behaviours subsequently recovered and there was no effect of treatment on nest building success. The potential causes and implications of these findings are discussed