A Model of System Effectiveness and its Application to Educational Organizations

Working Paper Serie

When career paths cease to exist : a qualitative study of career behavior in a crisis economy

Using grounded theory methodology, this study examines the ways young professionals describe their career paths in the aftermath of the 2008 financial crisis. We interviewed a sample of 29 Greek women professionals (24 to 32 years old) to examine their career behavior during this recession. Findings reveal prevailing effects of professional identity and profession-consistent learning goals on participants’ career behavior. Specifically, those individuals without a strong professional identity or profession-consistent learning goals are more likely to anticipate and engage in career activities unrelated to their professions, a group whom we refer to as Shifters. In contrast, Sustainers, a group having strong career identity and profession-focused learning, are far more likely to anticipate and engage in career activities tied to their profession. Based on these findings, we develop postulates regarding career behavior in contexts of severe austerity and recession where conventional career paths have broken down.© 2015. The attached document (embargoed until 28/09/2015) is an author produced version of a paper published in the Journal of Vocational Behavior, uploaded in accordance with the publisher’s self- archiving policy. The final published version (version of record) is available online at https://doi.org/10.1016/j.jvb.2015.09.009. Some minor differences between this version and the final published version may remain. We suggest you refer to the final published version should you wish to cite from it

Characterization of two polyvalent phages infecting Enterobacteriaceae

Bacteriophages display remarkable genetic diversity and host specificity. In this study, we explore phages infecting bacterial strains of the Enterobacteriaceae family because of their ability to infect related but distinct hosts. We isolated and characterized two novel virulent phages, SH6 and SH7, using a strain of Shigella flexneri as host bacterium. Morphological and genomic analyses revealed that phage SH6 belongs to the T1virus genus of the Siphoviridae family. Conversely, phage SH7 was classified in the T4virus genus of the Myoviridae family. Phage SH6 had a short latent period of 16 min and a burst size of 103 ± 16 PFU/infected cell while the phage SH7 latent period was 23 min with a much lower burst size of 26 ± 5 PFU/infected cell. Moreover, phage SH6 was sensitive to acidic conditions (pH < 5) while phage SH7 was stable from pH 3 to 11 for 1 hour. Of the 35 bacterial strains tested, SH6 infected its S. flexneri host strain and 8 strains of E. coli. Phage SH7 lysed additionally strains of E. coli O157:H7, Salmonella Paratyphi, and Shigella dysenteriae. The broader host ranges of these two phages as well as their microbiological properties suggest that they may be useful for controlling bacterial populations

Does what happens abroad stay abroad? Displaced aggression and emotional regulation in expatriate psychological contracts

The effects of psychological contract violation are the subject of considerable research. Yet, their effects in work arrangements with more than two parties are largely unknown. Multi‐party work arrangements differ from traditional ones because individuals may be vulnerable to psychological contract breach and violation by more than one party, potentially directing negative emotional responses not only towards the responsible party but also displacing it to the other (innocent) party. Primary data from a two‐wave survey of 221 current expatriates is used to test the effects of displaced aggression and emotion regulation in multi‐party psychological contracts. We find that the negative emotions (violation experiences) associated with breach predict reduced commitment both to the perpetrating organization and the innocent party. However, this spillover effect is asymmetric and follows displaced aggregation theory: Expatriates displace their aggressive behaviour on to the host when the home organization violated the psychological contract, not the reverse

When Foreign Waves Hit Home Shores: Organizational Identification in Psychological Contract Breach‐Violation Relationships During International Assignments

In the context of international assignments, this study investigates the psychological contract breach-violation relationship from a multi-party employment perspective. Multi-party employment refers to arrangements where employees have concurrent psychological contracts with more than one party. Drawing on two-waves of survey data from 221 expatriates, we find both direct relationships and asymmetric spillover effects of psychological contract breach on violation. Psychological contract breach by either the home or host organization is directly linked to psychological contract violation by the breaching party. Additionally, spillover effects occur such that a breach by the host predicts psychological contract violation by the home organization, though not the reverse. These relationships are shaped by the expatriates’ organizational identification. Identification with the host buffers the direct effect between breach and violation by the host, while dual organizational identification mitigates the direct effect between breach and violation by the home organization. Identification with the home organization diminishes the spillover effect from host breach to home organization violation. The opposite, identification with the host, amplifies the spillover effect of host breach to home organization violation. By examining the distinct dynamics of home and host organization contract breach and violation, we develop theoretical implications for understanding PCs in multi-party work arrangements

Characterization of CRISPR-Cas systems in the Ralstonia solanacearum species complex

Clustered regularly interspaced short palindromic repeats (CRISPRs) are composed of an array of short DNA repeat sequences separated by unique spacer sequences that are flanked by associated (Cas) genes. CRISPR‐Cas systems are found in the genomes of several microbes and can act as an adaptive immune mechanism against invading foreign nucleic acids, such as phage genomes. Here, we studied the CRISPR‐Cas systems in plant‐pathogenic bacteria of the Ralstonia solanacearum species complex (RSSC). A CRISPR‐Cas system was found in 31% of RSSC genomes present in public databases. Specifically, CRISPR‐Cas types I‐E and II‐C were found, with I‐E being the most common. The presence of the same CRISPR‐Cas types in distinct Ralstonia phylotypes and species suggests the acquisition of the system by a common ancestor before Ralstonia species segregation. In addition, a Cas1 phylogeny (I‐E type) showed a perfect geographical segregation of phylotypes, supporting an ancient acquisition. Ralstoniasolanacearum strains CFBP2957 and K60T were challenged with a virulent phage, and the CRISPR arrays of bacteriophage‐insensitive mutants (BIMs) were analysed. No new spacer acquisition was detected in the analysed BIMs. The functionality of the CRISPR‐Cas interference step was also tested in R. solanacearum CFBP2957 using a spacer‐protospacer adjacent motif (PAM) delivery system, and no resistance was observed against phage phiAP1. Our results show that the CRISPR‐Cas system in R. solanacearum CFBP2957 is not its primary antiviral strategy

Complete genome sequence of Escherichia coli Siphophage BRET

The lytic Escherichia coli siphophage BRET was isolated from a chicken obtained at a local market in Abidjan, Côte d’Ivoire. Its linear genome sequence consists of 59,550 bp (43.4% GC content) and contains 88 predicted genes, including 4 involved in archaeosine biosynthesis. Phage BRET is related (95% nucleotide identity) to Enterobacteria phage JenK