Hsc70-induced changes in clathrin-auxilin cage structure suggest a role for clathrin light chains in cage disassembly

The molecular chaperone, Hsc70, together with its co-factor, auxilin, facilitates the ATP-dependent removal of clathrin during clathrin-mediated endocytosis in cells. We have used cryo-electron microscopy to determine the 3D structure of a complex of clathrin, auxilin401-910 and Hsc70 at pH 6 in the presence of ATP, frozen within 20 seconds of adding Hsc70 in order to visualize events that follow the binding of Hsc70 to clathrin and auxilin before clathrin disassembly. In this map, we observe density beneath the vertex of the cage that we attribute to bound Hsc70. This density emerges asymmetrically from the clathrin vertex, suggesting preferential binding by Hsc70 for one of the three possible sites at the vertex. Statistical comparison with a map of whole auxilin and clathrin previously published by us reveals the location of statistically significant differences which implicate involvement of clathrin light chains in structural rearrangements which occur after Hsc70 is recruited. Clathrin disassembly assays using light scattering suggest that loss of clathrin light chains reduces the efficiency with which auxilin facilitates this reaction. These data support a regulatory role for clathrin light chains in clathrin disassembly in addition to their established role in regulating clathrin assembly

Crop Updates 2005 Oilseeds

This session covers fifteen papers from different authors: 1. ACKNOWLEDGEMENTS, Douglas Hamilton, FARMING SYSTEMS DEVELOPMENT OFFICER CROP AGRONOMY AND NUTRITION 2. Canola workshop at Crop Updates 2005, Oilseeds WA, John Duff, EXECUTIVE OFFICER OILSEEDS WA 3. Comparison of IT and TT canola varieties in geographic zones of WA, 2003-4, Graham Walton and Hasan Zaheer, Department of Agriculture 4. Farmer scale canola variety trials in WA, 2004, Graham Walton, John Duff, Neil Harris and Heather Cosgriff, Oilseeds WA 5. Oilseed crops for industrial uses, Margaret C. Campbell, Centre for Legumes in Mediterranean Agriculture (CLIMA), Graham Walton,Department of Agriculture 6. Weed control opportunities with GM canola, Bill Crabtree, Independent Consultant, Northam 7. Soil and tissue tests for the sulfur requirements of canola, R.F. Brennan and M.D.A. Bolland, Department of Agriculture 8. Tests to predict the potassium requirements of canola, R.F. Brennan and M.D.A. Bolland, Department of Agriculture 9. Genotypic variation in potassium efficiency of canola, P.M. Damon and Z. Rengel, Faculty of Natural and Agricultural Sciences, UWA 10. Atrazine contamination of groundwater in the agricultural region of Western Australia, Russell Speed1, Neil Rothnie2, John Simons1, Ted Spadek2 and John Moore1;1Department of Agriculture, 2Chemistry Centre (WA) PESTS AND DISEASES 11. Controlling aphids and Beet western yellows virus in canola using imidacloprid seed dressing, Brenda Coutts and Roger Jones; Department of Agriculture 12. Managing sclerotinia in canola, Neil Harris, Dovuro Seeds Western Australia 13. Slugs, the trail of destruction in canola, Neil Harris, Dovuro Seeds Western Australia 14. Blackleg risk assessment and strategies for risk management in canola during 2005 and beyond, Moin Salam, Ravjit Khanguraand Art Diggle, Department of Agriculture 15. Modelling: BRAT – Blackleg Risk Appraisal Tool, Moin Salam, Ravjit KhanguraDepartment of Agricultur

Crop Updates 2006 - Weeds

This session covers thirty seven papers from different authors: 1. ACKNOWLEDGEMENTS, Alexandra Douglas, CONVENOR – WEEDS DEPARTMENT OF AGRICULTURE SPRAY TECHNOLOGY 2. Meeting the variable application goals with new application technology, Thomas M. Wolf, Agriculture and Agri-Food Canada, Saskatoon Research Centre 3. Spray nozzles for grass weed control, Harm van Rees, BCG (Birchip Cropping Group) 4. Boom sprayer setups – achieving coarse droplets with different operating parameters, Bill Gordon, Bill Gordon Consulting 5. Complying with product label requirements, Bill Gordon, Bill Gordon Consulting 6. IWM a proven performer over 5 years in 33 focus paddocks, Peter Newman and Glenn Adam, Department of Agriculture 7. Crop topping of wild radish in lupins and barley, how long is a piece of string? Peter Newman and Glenn Adam, Department of Agriculture 8. Determining the right timing to maximise seed set control of wild radish, Aik Cheam and Siew Lee, Department of Agriculture 9. Why weed wiping varies in success rates in broadacre crops? Aik Cheam1, Katherine Hollaway2, Siew Lee1, Brad Rayner1 and John Peirce1,1Department of Agriculture, 2Department of Primary Industries, Victoria 10. Are WA growers successfully managing herbicide resistant annual ryegrass? Rick Llewellynabc, Frank D’Emdena, Mechelle Owenb and Stephen Powlesb aCRC Australian Weed Management, School of Agricultural and Resource Economics, University of Western Australia; bWA Herbicide Resistance Initiative, University of Western Australia. cCurrent address: CSIRO Sustainable Ecosystems 11. Do herbicide resistant wild radish populations look different? Michael Walsh, Western Australian Herbicide Resistance Initiative, University of Western Australia 12. Can glyphosate and paraquat annual ryegrass reduce crop topping efficacy? Emma Glasfurd, Michael Walsh and Kathryn Steadman, Western Australian Herbicide Resistance Initiative, University of Western Australia 13. Tetraploid ryegrass for WA. Productive pasture phase AND defeating herbicide resistant ryegrass, Stephen Powlesa, David Ferrisab and Bevan Addisonc, aWA Herbicide Resistance Initiative, University of Western Australia; bDepartment of Agriculture, and cElders Limited 14. Long-term management impact on seedbank of wild radish with multiple resistance to diflufenican and triazines, Aik Cheam, Siew Lee, Dave Nicholson and Ruben Vargas, Department of Agriculture 15. East-west crop row orientation improves wheat and barley yields, Dr Shahab Pathan, Dr Abul Hashem, Nerys Wilkins and Catherine Borger3, Department of Agriculture, 3WAHRI, The University ofWestern Australia 16. Competitiveness of different lupin cultivars with wild radish, Dr Shahab Pathan, Dr Bob French and Dr Abul Hashem, Department of Agriculture 17. Managing herbicide resistant weeds through farming systems, Kari-Lee Falconer, Martin Harries and Chris Matthews, Department of Agriculture 18. Lupins tolerate in-row herbicides well, Peter Newman and Martin Harries, Department of Agriculture 19. Summer weeds can reduce wheat grain yield and protein, Dr Abul Hashem1, Dr Shahab Pathan1 and Vikki Osten3, 1Department Agriculture, 3Senior Agronomist, CRC for Australian Weed Management, Queensland Department of Primary Industries and Fisheries 20. Diuron post-emergent in lupins, the full story, Peter Newman and Glenn Adam, Department of Agriculture 21. Double incorporation of trifluralin, Peter Newman and Glenn Adam, Department of Agriculture 22. Herbicide tolerance of narrow leafed and yellow lupins, Harmohinder Dhammu, David Nicholson, Department of Agriculture 23. MIG narrow leaf lupin herbicide tolerance trial, Richard Quinlan, Planfarm Pty Ltd, Trials Coordinator MIG; Debbie Allen, Research Agronomist – MIG 24. Herbicide tolerance of new albus lupins, Harmohinder Dhammu, David Nicholson, Department of Agriculture 25. Field pea x herbicide tolerance, Mark Seymour and Harmohinder Dhammu, Research Officers, and Pam Burgess, Department of Agriculture 26. Faba bean variety x herbicide tolerance, Mark Seymour and Harmohinder Dhammu, Research Officers, and Pam Burgess, Department of Agriculture 27. Herbicide tolerance of new Kabili chickpeas, Harmohinder Dhammu, Owen Coppen and Chris Roberts, Department of Agriculture 28. Timing of phenoxys application in EAG Eagle Rock, Harmohinder Dhammu, David Nicholson, Department of Agriculture 29. Herbicide tolerance of new wheat varieties, Harmohinder Dhammu, David Nicholson, Department of Agriculture 30. Lathyrus sativus x herbicide tolerance, Mark Seymour, Department of Agriculture 31. Tolerance of annual pasture species to herbicides and mixtures containing diuron, Christiaan Valentine and David Ferris, Department of Agriculture 32. The impact of herbicides on pasture legume species – a summary of scientific trial results across 8 years, Christiaan Valentine and David Ferris, Department of Agriculture 33. The impact of spraytopping on pasture legume seed set, Christiaan Valentine and David Ferris, Department of Agriculture 34. Ascochyta interaction with Broadstrike in chickpeas, H.S. Dhammu1, A.K. Basandrai2,3, W.J. MacLeod1, 3 and C. Roberts1, 1Department of Agriculture, 2CSKHPAU, Dhaulakuan, Sirmour (HP), India and 3CLIMA 35. Best management practices for atrazine in broadacre crops, John Moore, Department of Agriculture, Neil Rothnie, Chemistry Centre of WA, Russell Speed, Department of Agriculture, John Simons, Department of Agriculture, and Ted Spadek, Chemistry Centre of WA 36. Biology and management of red dodder (Cuscuta planiflolia) – a new threat to the grains industry, Abul Hashem, Daya Patabendige and Chris Roberts, Department Agriculture 37. Help the wizard stop the green invaders! Michael Renton, Sally Peltzer and Art Diggle, Department of Agricultur

Crop Updates 2005 - Geraldton

This session covers seventeen papers from different authors 2005 Seasonal Outlook, David Stephens and Nicola Telcik, Department of Agriculture Horses for Courses – using the best tools to manage climate risk, Cameron Weeks, Mingenew-Irwin Group / Planfarm and Richard Quinlan, Planfarm Agronomy Global influences driving Australian agriculture, Tony Harman, Australian Government Department of Agriculture, Fisheries and Forestry Wheat yield and quality improvements – where have they come from and can we have more? Wal Anderson, Department of Agriculture Rotations for nematode management, Vivien A. Vanstone, Sean J. Kelly, Helen F. Hunter and Mena C. Gilchrist, Department of Agriculture Integrate strategies to manage stripe rust risk, Ciara Beard, Geoff Thomas, Robert Loughman, Kith Jayasena and Manisha Shenkar, Department of Agriculture Frequency of herbicide resistance in wild radish populations across the WA wheatbelt, Dr Michael Walsh, Mechelle Owen and Prof. Stephen Powels, University of Western Australia The incidence and severity of wild radish resistance in the NAR – results from an in-situ survey, Rob Grima and Andrew Blake, Elders Limited Stubble management: the pros and cons of different methods, Bill Bowden, Department of Agriculture, Mike Collins WANTFA Effectiveness of Zinc Application Methods in Wheat, Luigi Moreschi, CSBP Know your Mo, Douglas Hamilton, Department of Agriculture Atrazine contamination of groundwater in the agricultural region of Western Australia, Russell Speed1, Neil Rothnie2, John Simons1, Ted Spadek2, and John Moore1, 1Department of Agriculture, 2Chemistry Centre (W.A.) Comparison of canola varieties in the Northern Agricultural Region in 2004, Graham Walton Department of Agriculture Pasture rotations are a promising option for sandplain production, Nadine Eva, Department of Agriculture Zone management can improve profit on sandplain, Bindi Webb, Damian Shepherd, Department of Agriculture, David Forrester, Davejeff Farms, casuarinas via Geraldton, Peter Tozer, Department of Agriculture Crop leftovers: what’s in stubble for sheep? Roy Butler and Keith Croker, Department of Agriculture Realising Rural Equity, Nathan Windebank, Australian Agricultural Contracts Limite

Closing the mortality gap after a myocardial infarction in people with and without chronic obstructive pulmonary disease.

OBJECTIVE: Patients with chronic obstructive pulmonary disease (COPD) have increased mortality following myocardial infarction (MI) compared with patients without COPD. We investigated the extent to which differences in recognition and management after MI could explain the mortality difference. METHODS: 300 161 patients with a first MI between 2003 and 2013 were identified in the UK Myocardial Ischaemia National Audit Project database. Logistic regression was used to compare mortality in hospital and at 180 days postdischarge between patients with and without COPD. Variables relating to inhospital factors (delay in diagnosis, use of reperfusion and time to reperfusion/use of angiography) and use of secondary prevention were sequentially added to models. RESULTS: Mortality was higher for patients with COPD both inhospital (4.6% vs 3.2%) and at 180 days (12.8% vs 7.7%). After adjusting for inhospital factors, the effect of COPD on inhospital mortality after MI was reduced for both ST-elevation myocardial infarctions (STEMIs) and non-STEMIs (STEMIs OR 1.24 (95% CI 1.10 to 1.41) to 1.13 (95% CI 0.99 to 1.29); non-STEMIs OR 1.34 (95% CI 1.24 to 1.45) to 1.16 (95% CI 1.07 to 1.26)). Adjusting for inhospital factors reduced the effect of COPD on mortality after non-STEMI at 180 days (OR 1.56 (95% CI 1.47 to 1.65) to 1.37 (95% CI 1.31 to 1.44)). Adjusting for use of secondary prevention also reduced the effect of COPD on mortality at 180 days for STEMIs and non-STEMIs (STEMIs OR 1.45 (95% CI 1.31 to 1.61) to 1.25 (95% CI 1.11 to 1.41); non-STEMIs OR 1.37 (95% CI 1.31 to 1.44) to 1.26 (95% CI 1.17 to 1.35). CONCLUSIONS: Delayed diagnosis, timing and use of reperfusion of a STEMI, use of angiography after a non-STEMI and use of secondary prevention medicines are all potential explanations for the mortality gap after MI in people with COPD

Predicting mortality after acute coronary syndromes in people with chronic obstructive pulmonary disease.

OBJECTIVE: To assess the accuracy of Global Registry of Acute Coronary Events (GRACE) scores in predicting mortality at 6 months for people with chronic obstructive pulmonary disease (COPD) and to investigate how it might be improved. METHODS: Data were obtained on 481 849 patients with acute coronary syndrome admitted to UK hospitals between January 2003 and June 2013 from the Myocardial Ischaemia National Audit Project (MINAP) database. We compared risk of death between patients with COPD and those without COPD at 6 months, adjusting for predicted risk of death. We then assessed whether several modifications improved the accuracy of the GRACE score for people with COPD. RESULTS: The risk of death after adjusting for GRACE score predicted that risk of death was higher for patients with COPD than that for other patients (RR 1.29, 95% CI 1.28 to 1.33). Adding smoking into the GRACE score model did not improve accuracy for patients with COPD. Either adding COPD into the model (relative risk (RR) 1.00, 0.94 to 1.02) or multiplying the GRACE score by 1.3 resulted in better performance (RR 0.99, 0.96 to 1.01). CONCLUSIONS: GRACE scores underestimate risk of death for people with COPD. A more accurate prediction of risk of death can be obtained by adding COPD into the GRACE score equation, or by multiplying the GRACE score predicted risk of death by 1.3 for people with COPD. This means that one third of patients with COPD currently classified as low risk should be classified as moderate risk, and could be considered for more aggressive early treatment after non-ST-segment elevation myocardial infarction or unstable angina

Crop Updates 2005 Oilseeds

This session covers fifteen papers from different authors: 1. ACKNOWLEDGEMENTS, Douglas Hamilton, FARMING SYSTEMS DEVELOPMENT OFFICER CROP AGRONOMY AND NUTRITION 2. Canola workshop at Crop Updates 2005, Oilseeds WA, John Duff, EXECUTIVE OFFICER OILSEEDS WA 3. Comparison of IT and TT canola varieties in geographic zones of WA, 2003-4, Graham Walton and Hasan Zaheer, Department of Agriculture 4. Farmer scale canola variety trials in WA, 2004, Graham Walton, John Duff, Neil Harris and Heather Cosgriff, Oilseeds WA 5. Oilseed crops for industrial uses, Margaret C. Campbell, Centre for Legumes in Mediterranean Agriculture (CLIMA), Graham Walton,Department of Agriculture 6. Weed control opportunities with GM canola, Bill Crabtree, Independent Consultant, Northam 7. Soil and tissue tests for the sulfur requirements of canola, R.F. Brennan and M.D.A. Bolland, Department of Agriculture 8. Tests to predict the potassium requirements of canola, R.F. Brennan and M.D.A. Bolland, Department of Agriculture 9. Genotypic variation in potassium efficiency of canola, P.M. Damon and Z. Rengel, Faculty of Natural and Agricultural Sciences, UWA 10. Atrazine contamination of groundwater in the agricultural region of Western Australia, Russell Speed1, Neil Rothnie2, John Simons1, Ted Spadek2 and John Moore1;1Department of Agriculture, 2Chemistry Centre (WA) PESTS AND DISEASES 11. Controlling aphids and Beet western yellows virus in canola using imidacloprid seed dressing, Brenda Coutts and Roger Jones; Department of Agriculture 12. Managing sclerotinia in canola, Neil Harris, Dovuro Seeds Western Australia 13. Slugs, the trail of destruction in canola, Neil Harris, Dovuro Seeds Western Australia 14. Blackleg risk assessment and strategies for risk management in canola during 2005 and beyond, Moin Salam, Ravjit Khanguraand Art Diggle, Department of Agriculture 15. Modelling: BRAT – Blackleg Risk Appraisal Tool, Moin Salam, Ravjit KhanguraDepartment of Agricultur

Deutsch-jüdische Bibliografie – Digital, vernetzt und erforschbar?

Improved methods have been devised for the isolation of (+)-conocurvone (1) from the roots of Conospermum brachyphyllum Lindl. and for its synthesis. The isolation of (R)-(+)-9-hydroxy-3-(4′-hydroxy-4′-methylpentan-1′-yl)-3,8-dimethyl-3H-naphtho[2,1-b]pyran-7,10-dione [(+)-brachyphyllone] (2), a minor constituent of this species, its structural elucidation and partial synthesis are also described. 8-C-Methylteretifolione-B (9) and 8-C-methyl-9-O-methylteretifolione-B (10), as well as the previously isolated (+)-teretifolione-B (8), also occur in this plant