24 research outputs found
THE INFLUENCE OF TECHNOLOGICAL ELEMENTS ON THE WINTER TRITICALE YIELD
The paper presents the 4-year data on the behaviour of local winter triticale varieties Ingen 35 and Ingen 93 in multifactorial field experiments. The highest yields of winter triticale varieties were recorded after the forerunner grain peas: Ingen 35 - 3632 kg/ha and Ingen 93 - 3744 kg/ha. Referring to the planting dates, the highest yields were recorded on the optimal planting dates. Ingen 35 variety provided yields at the level of 4265 kg/ha after the forerunner grain peas, 3448 kg/ha after the forerunner vetch-oat and 2770 kg/ha after the forerunner sunflower. The Ingen 93 variety recorded yields of 4176 kg/ha after the forerunner grain peas, 3778 kg/ha after vetch-oat and 2929 kg/ha after the forerunner sunflower. In the case of the seeding rate, the highest yields were obtained for the variant 5.0 mln/ha, which ensured 3161 kg/ha for the Ingen 93 variety and 3077 kg/ha for the Ingen 35 variety. The dominant influence degree on the grain production belongs to the forerunner crop: 67.11% for Ingen 35 and 73.16% for Ingen 93. The influence of the planting dates was of 31.92% and 25.56% respectively for the varieties. The highest percentage of protein content was recorded after the forerunner grain peas β 14.11% for the Ingen 93 variety. It was established the tendency to increase the protein content from the optimal to the late planting dates for both varieties and after all forerunner crops.The highest protein yield was recorded after the forerunner grain peas in both varieties: 426.1 kg/ha for Ingen 35 and 435.5 kg/ha for Ingen 93
Inhibition of cathepsin B by caspase-3 inhibitors blocks programmed cell death in <i>Arabidopsis</i>
Programmed cell death (PCD) is used by plants for development and survival to biotic and abiotic stresses. The role of caspases in PCD is well established in animal cells. Over the past 15 years, the importance of caspase-3-like enzymatic activity for plant PCD completion has been widely documented despite the absence of caspase orthologues. In particular, caspase-3 inhibitors blocked nearly all plant PCD tested. Here, we affinity-purified a plant caspase-3-like activity using a biotin-labelled caspase-3 inhibitor and identified Arabidopsis thaliana cathepsin B3 (AtCathB3) by liquid chromatography with tandem mass spectrometry (LC-MS/MS). Consistent with this, recombinant AtCathB3 was found to have caspase-3-like activity and to be inhibited by caspase-3 inhibitors. AtCathepsin B triple-mutant lines showed reduced caspase-3-like enzymatic activity and reduced labelling with activity-based caspase-3 probes. Importantly, AtCathepsin B triple mutants showed a strong reduction in the PCD induced by ultraviolet (UV), oxidative stress (H2O2, methyl viologen) or endoplasmic reticulum stress. Our observations contribute to explain why caspase-3 inhibitors inhibit plant PCD and provide new tools to further plant PCD research. The fact that cathepsin B does regulate PCD in both animal and plant cells suggests that this protease may be part of an ancestral PCD pathway pre-existing the plant/animal divergence that needs further characterisation
Π‘ΠΠΠΠΠΠΠ ΠΠΠΠ«Π₯ Π€ΠΠ Π Π¨Π’ΠΠΠΠΠΠΠΠ Π’ΠΠΠΠ’Π Π ΠΠ₯ ΠΠ‘ΠΠΠΠ¬ΠΠΠΠΠΠΠ Π Π‘ΠΠΠΠΠ¦ΠΠ
Tree-shaped plants of tomato have many useful traits, which gain them advantages over common tomato forms. In world plant collection there are over 200 accessions, cultivars and hybrids of tree-shaped tomato. The aim of the study was to develop new initial tomato accessions for breeding program for tree-shaped cultivars and hybrids that are distinguished by a shape, fruit weight and color, improved by interspecific hybridization chemical composition and resistance to the major diseases. The tree-shaped breeding lines: β196/12β, β374/08β, β909/14β, β911/14β and other were observed with the use of interspecific hybridization and selection in the population F2-F4. The selected lines passed the trial in artificially infected condition with Alternaria, viral diseases (MToV, TSWV) and phytoplasmas. Among breeding lines assessed the β911/14β, β374/08β andβ40/11β were less affected by complex of pathogens. The lines β196/12β and β909/14β passed an assessment in the nursery for variety trial in naturally and Β provocatively infected conditions with major pathogens. Both breeding lines had an advantage over standard accession βLagunaβ and βMaraphonβ for total yield capacity and standard fruit harvest; it was more by 9.6% and 52.2% and 9.5% and 53.4% respectively. Fruits of lines 196/12 and 909/14 had good taste quality with high content of dry matter (5.8% and 6.8%), sugars (3.3% and 3.1%), vitamin C (22.2 and 24.8 mg/100 grams). The selected tree-shaped accessions have been used to develop heterotic hybrids and to be sources of economically valuable traits.Π¨ΡΠ°ΠΌΠ±ΠΎΠ²ΡΠ΅Β Β ΡΠ°ΡΡΠ΅Π½ΠΈΡΒ ΡΠΎΠΌΠ°ΡΠ° ΠΈΠΌΠ΅ΡΡΒ ΡΡΠ΄ ΠΏΠΎΠ»Π΅Π·Π½ΡΡ
ΠΏΡΠΈΠ·Π½Π°ΠΊΠΎΠ², ΠΊΠΎΡΠΎΡΡΠ΅ ΡΠΎΠ·Π΄Π°ΡΡ ΠΈΠΌ ΠΏΡΠ΅ΠΈΠΌΡΡΠ΅ΡΡΠ²ΠΎ Π½Π°Π΄ ΠΎΠ±ΡΠΊΠ½ΠΎΠ²Π΅Π½Π½ΡΠΌΠΈΒ ΡΠ°ΡΡΠ΅Π½ΠΈΡΠΌΠΈ. Π ΠΌΠΈΡΠΎΠ²ΠΎΠΉ ΠΊΠΎΠ»Π»Π΅ΠΊΡΠΈΠΈ ΡΠΎΠΌΠ°ΡΠ° Π½Π°ΡΡΠΈΡΡΠ²Π°Π΅ΡΡΡΒ Β Π±ΠΎΠ»Π΅Π΅ 200 ΡΡΠ°ΠΌΠ±ΠΎΠ²ΡΡ
ΡΠΎΡΡΠΎΠ² ΠΈ Π³ΠΈΠ±ΡΠΈΠ΄ΠΎΠ². Π¦Π΅Π»ΡΡ Π½Π°ΡΠΈΡ
ΠΈΡΡΠ»Π΅Π΄ΠΎΠ²Π°Π½ΠΈΠΉ Π±ΡΠ»ΠΎ ΡΠΎΠ·Π΄Π°Π½ΠΈΠ΅ Π½ΠΎΠ²ΠΎΠ³ΠΎ ΠΈΡΡ
ΠΎΠ΄Π½ΠΎΠ³ΠΎΒ ΠΌΠ°ΡΠ΅ΡΠΈΠ°Π»Π° Π΄Π»Ρ ΡΠ΅Π»Π΅ΠΊΡΠΈΠΈΒ ΡΡΠ°ΠΌΠ±ΠΎΠ²ΡΡ
ΡΠΎΡΡΠΎΠ² ΠΈΒ Π³ΠΈΠ±ΡΠΈΠ΄ΠΎΠ² ΡΠΎΠΌΠ°ΡΠ°, ΠΎΡΠ»ΠΈΡΠ°ΡΡΠΈΠ΅ΡΡΒ Β ΡΠΎΡΠΌΠΎΠΉ, ΠΌΠ°ΡΡΠΎΠΉ ΠΈ ΠΎΠΊΡΠ°ΡΠΊΠΎΠΉ ΠΏΠ»ΠΎΠ΄Π°, ΡΠ»ΡΡΡΠ΅Π½ΠΈΠ΅ ΠΏΡΡΠ΅ΠΌΒ ΠΌΠ΅ΠΆΠ²ΠΈΠ΄ΠΎΠ²ΠΎΠΉ Π³ΠΈΠ±ΡΠΈΠ΄ΠΈΠ·Π°ΡΠΈΠΈ, Ρ
ΠΈΠΌΠΈΡΠ΅ΡΠΊΠΎΠ³ΠΎΒ ΡΠΎΡΡΠ°Π²Π° ΠΏΠ»ΠΎΠ΄ΠΎΠ² ΠΈ ΠΏΠΎΠ²ΡΡΠ΅Π½ΠΈΠ΅ ΡΡΡΠΎΠΉΡΠΈΠ²ΠΎΡΡΠΈ ΠΊ ΠΎΡΠ½ΠΎΠ²Π½ΡΠΌ Π±ΠΎΠ»Π΅Π·Π½ΡΠΌ. Π‘ ΠΈΡΠΏΠΎΠ»ΡΠ·ΠΎΠ²Π°Π½ΠΈΠ΅ΠΌ ΠΌΠ΅ΠΆΠ²ΠΈΠ΄ΠΎΠ²ΠΎΠΉ Π³ΠΈΠ±ΡΠΈΠ΄ΠΈΠ·Π°ΡΠΈΠΈ ΠΈ ΠΌΠ΅ΡΠΎΠ΄ΠΎΠΌ ΠΎΡΠ±ΠΎΡΠ° Π² Π³ΠΈΠ±ΡΠΈΠ΄Π½ΡΡ
ΠΏΠΎΠΏΡΠ»ΡΡΠΈΡΡ
F2-F4 ΠΏΠΎΠ»ΡΡΠ΅Π½Ρ ΡΡΠ°ΠΌΠ±ΠΎΠ²ΡΠ΅Β Π»ΠΈΠ½ΠΈΠΈΒ ΡΠΎΠΌΠ°ΡΠ°Β Β (196/12, 374/08,Β 909/14,Β 911/14Β ΠΈΒ Π΄Ρ.). ΠΡΠ΄Π΅Π»Π΅Π½Π½ΡΠ΅ Π»ΠΈΠ½ΠΈΠΈ ΠΏΡΠΎΡΠ»ΠΈ ΠΎΡΠ΅Π½ΠΊΡ Π½Π° ΠΈΡΠΊΡΡΡΡΠ²Π΅Π½Π½ΡΡ
ΠΈΠ½ΡΠ΅ΠΊΡΠΈΠΎΠ½Π½ΡΡ
ΡΠΎΠ½Π°Ρ
Π°Π»ΡΡΠ΅ΡΠ½Π°ΡΠΈΠΎΠ·Π°,Β Π²ΠΈΡΡΡΠ½ΡΡ
Π±ΠΎΠ»Π΅Π·Π½Π΅ΠΉ (ΠΠΠ’ΠΎ, ΠΠΠ’) ΠΈ ΡΠΈΡΠΎΠΏΠ»Π°Π·ΠΌΠΎΠ·Π°.Β Π‘ΡΠ΅Π΄ΠΈ ΠΎΡΠ΅Π½Π΅Π½Π½ΡΡ
ΠΎΠ±ΡΠ°Π·ΡΠΎΠ² Π½Π°ΠΈΠΌΠ΅Π½ΡΡΠ΅ΠΉΒ ΠΏΠΎΡΠ°ΠΆΠ°Π΅ΠΌΠΎΡΡΡΡΒ ΠΊΠΎΠΌΠΏΠ»Π΅ΠΊΡΠΎΠΌ ΠΏΠ°ΡΠΎΠ³Π΅Π½ΠΎΠ²Β ΠΎΡΠ»ΠΈΡΠ°Π»ΠΈΡΡΒ Β Π»ΠΈΠ½ΠΈΠΈ 911/14, 374/08 ΠΈ 40/11. Π 2011-2012 Π³ΠΎΠ΄Π°Ρ
Π»ΠΈΠ½ΠΈΠΈ 196/12 ΠΈ 909/14 ΠΏΡΠΎΡΠ»ΠΈ ΠΎΡΠ΅Π½ΠΊΡ Π² ΠΏΠΈΡΠΎΠΌΠ½ΠΈΠΊΠ΅ ΠΊΠΎΠ½ΠΊΡΡΡΠ½ΠΎΠ³ΠΎ ΡΠΎΡΡΠΎΠΈΡΠΏΡΡΠ°Π½ΠΈΡ Π½Π° Π΅ΡΡΠ΅ΡΡΠ²Π΅Π½Π½ΠΎΠΌ ΠΏΡΠΎΠ²ΠΎΠΊΠ°ΡΠΈΠΎΠ½Π½ΠΎΠΌ ΡΠΎΠ½Π΅ ΠΎΡΠ½ΠΎΠ²Π½ΡΡ
Π±ΠΎΠ»Π΅Π·Π½Π΅ΠΉ. ΠΠ±Π΅ Π»ΠΈΠ½ΠΈΠΈ ΠΈΠΌΠ΅Π»ΠΈ ΠΏΡΠ΅ΠΈΠΌΡΡΠ΅ΡΡΠ²ΠΎ Π½Π°Π΄ ΡΡΠ°Π½Π΄Π°ΡΡΠ°ΠΌΠΈ ΠΠ°Π³ΡΠ½Π° ΠΈ ΠΠ°ΡΠ°ΡΠΎΠ½ ΠΏΠΎ ΠΎΠ±ΡΠ΅ΠΉ ΡΡΠΎΠΆΠ°ΠΉΠ½ΠΎΡΡΠΈ (Π½Π° 9,6 ΠΈ 52,2% ΡΠΎΠΎΡΠ²Π΅ΡΡΡΠ²Π΅Π½Π½ΠΎ) ΠΈ Π²ΡΡ
ΠΎΠ΄ΡΒ ΡΡΠ°Π½Π΄Π°ΡΡΠ½ΡΡ
ΠΏΠ»ΠΎΠ΄ΠΎΠ² (9,5 ΠΈ 53,4% ΡΠΎΠΎΡΠ²Π΅ΡΡΡΠ²Π΅Π½Π½ΠΎ).Β ΠΠ»ΠΎΠ΄Ρ Π»ΠΈΠ½ΠΈΠΉΒ Β 196/12 ΠΈ 909/14 ΠΎΠ±Π»Π°Π΄Π°Π»ΠΈ Ρ
ΠΎΡΠΎΡΠΈΠΌΠΈ Π²ΠΊΡΡΠΎΠ²ΡΠΌΠΈ ΠΊΠ°ΡΠ΅ΡΡΠ²Π°ΠΌΠΈ,Β Π²ΡΡΠΎΠΊΠΈΠΌ ΡΠΎΠ΄Π΅ΡΠΆΠ°Π½ΠΈΠ΅ΠΌ ΡΡΡ
ΠΈΡ
Π²Π΅ΡΠ΅ΡΡΠ² (5,8%; 6,8%), ΡΠ°Ρ
Π°ΡΠΎΠ² (3,3%; 3,1%), Π²ΠΈΡΠ°ΠΌΠΈΠ½Π° Π‘ (22,2; 24,8 ΠΌΠ³/100 Π³ ΡΠΎΠΎΡΠ²Π΅ΡΡΡΠ²Π΅Π½Π½ΠΎ).Β ΠΡΠ΄Π΅Π»ΠΈΠ²ΡΠΈΠ΅ΡΡ ΡΡΠ°ΠΌΠ±ΠΎΠ²ΡΠ΅ ΠΎΠ±ΡΠ°Π·ΡΡΒ ΡΠΎΠΌΠ°ΡΠ° ΠΈΡΠΏΠΎΠ»ΡΠ·ΠΎΠ²Π°Π½Ρ ΠΊΠ°ΠΊ ΠΈΡΡ
ΠΎΠ΄Π½ΡΠΉ ΠΌΠ°ΡΠ΅ΡΠΈΠ°Π» Π΄Π»ΡΒ ΡΠΎΠ·Π΄Π°Π½ΠΈΡ Π³Π΅ΡΠ΅ΡΠΎΠ·ΠΈΡΠ½ΡΡ
Β Π³ΠΈΠ±ΡΠΈΠ΄ΠΎΠ² ΠΈΒ ΠΊΠ°ΠΊΒ Π³Π΅Π½Π΅ΡΠΈΡΠ΅ΡΠΊΠΈΠΉ ΠΈΡΡΠΎΡΠ½ΠΈΠΊ Ρ
ΠΎΠ·ΡΠΉΡΡΠ²Π΅Π½Π½ΠΎ ΡΠ΅Π½Π½ΡΡ
ΠΏΡΠΈΠ·Π½Π°ΠΊΠΎΠ²
PRINCIPLES OF THE DETERMINATION ENERGY INTENSITY OF THE AGRICULTURAL PRODUCTS
A set of energy resources is consumed in the primary production and conversion process of agricultural product. Minimal quantity of energy required for the production of agricultural product unit is considered by the notion of energy intensity of agricultural products
Caspase-like activities and UV-induced programmed cell death in <i>Arabidopsis</i>
A very important goal is to determine which molecular components may be used in the execution of programmed cell death (PCD) in plants, which have been conserved during evolution and which are plant specific. Using A. thaliana we have shown that UV radiation can induce apoptotic-like changes at the cellular level and that an UV experimental system was relevant to the study of PCD in plants. UV induction of PCD requires light and a protease cleaving the caspase substrate Asp-Glu-Val-Asp (DEVDase activity) is induced within 30 minutes and peaks at one hour. This DEVDase appears related to animal caspases at the biochemical level, being insensitive to broad-range cysteine protease inhibitors. In addition, caspase1, caspase-3 inhibitors and the pancaspase inhibitor p35 were able to suppress DNA fragmentation and cell death. These results suggest that a YVADase (Tyr-Val-Ala-Asp) activity and an inducible DEVDase activity are possibly mediating DNA fragmentation during plant PCD induced by UV overexposure. Progress is being made towards the biochemical characterisation of the proteases involved
Hybrid technology for collective expertise
This paper outlines a new automatizated technology for collective expertise. The technology provides flexible framework of collective expertise, allows to bring it into accord with the Decision Making Person's conception of the problem, ensures alternative abilities for examination and interpretation of obtained expert information. Information about basic components of the instrumental system for collective expertise KIOT-1 is presented
Ring Cavity Tunable Fiber Laser With External Transversely Chirped Bragg Grating
Application of the Transverse Chirped Bragg Grating (TCBG - a reflecting volume Bragg grating with continuously variable resonant wavelength across the aperture) for the narrowband tunable ring-cavity fiber laser is presented. The main advantage of the use of TCBG is that its linear translation allows continuous tuning of emission wavelength within 5-10 nm band. Yb doped fiber laser operating in the wavelength range of 1050-1055 nm of narrowband emission up to 2.3 W is demonstrated. Β© 2013 SPIE