Supercritical technologies as sustainable tool for the concentration and co-encapsulation of Lavandula luisieri natural bioactives

Tesis inédita de la Universidad Complutense de Madrid, Facultad de Farmacia, leída el 27-01-2021Tradicionalmente, las plantas han sido utilizadas para el tratamiento y prevención de enfermedades o conservación de alimentos. Su diversa aplicación deriva de la producción de metabolitos secundarios como defensa contra agentes externos dañinos, mostrando una variada actividad con interés en diferentes campos. De hecho, la inclusión de plantas como herramienta terapéutica en los sistemas de salud está recomendada por organismos internacionales como la OMS. Actualmente el 52% de los fármacos son o bien un compuesto natural o un derivado del mismo, con un interés comercial creciente debido ala preferencia que los consumidores muestran los productos de origen natural como parte de hábitos de vida saludables para la prevención de enfermedades crónicas..Traditionally, plants have been used for the treatment and prevention of diseases or food conservation. These diverse applications are a consequence of the production of secondary metabolites as defence against external damaging agents, presenting a varied activity with interest in many fields. In fact, the inclusion of plants as therapeutical tool in health systems is recommended by international organisations like the WHO. Nowadays, 52% of drugs are a natural compound or a derivate, and have a growing commercial interest because of the consumers’ preference for natural products inclusion as part of natural habits to prevent chronic illnesses...Fac. de FarmaciaTRUEunpu

Supercritical Fluid fractionation of mentha suaveolens: concentration of functional properties

Supercritial Antisolvent Fractionation process was optimiced for the concentration of Mentha suaveolens bioactives in two different fractions. Their composition was analysed with HPLC. The antimicrobial activity of these fractions was assayed. The SAF process fractionated M. suaveolens tincture in two enriched fractions in different bioactives concentrating those with antimicrobial activity in one of them.  El proceso de Fraccionamiento Supercrítico Antidisolvente  fue optimizado para la concentrar bioactivos de Mentha suaveolens en dos diferentes fracciones. Su composición fue analizada con HPLC. La actividad antimicrobiana de estas fracciones fue ensayada. El proceso supercrítico permitió fraccionar la tintura de esta planta en 2 fracciones enrriqueciendolas en diferentes bioactivos y concentrando en uno de ellos los compuestos con actividad antimicrobiana.  &nbsp

Supercritical Fluid fractionation of mentha suaveolens: concentration of functional properties

Supercritial Antisolvent Fractionation process was optimiced for the concentration of Mentha suaveolens bioactives in two different fractions. Their composition was analysed with HPLC. The antimicrobial activity of these fractions was assayed. The SAF process fractionated M. suaveolens tincture in two enriched fractions in different bioactives concentrating those with antimicrobial activity in one of them.  El proceso de Fraccionamiento Supercrítico Antidisolvente  fue optimizado para la concentrar bioactivos de Mentha suaveolens en dos diferentes fracciones. Su composición fue analizada con HPLC. La actividad antimicrobiana de estas fracciones fue ensayada. El proceso supercrítico permitió fraccionar la tintura de esta planta en 2 fracciones enrriqueciendolas en diferentes bioactivos y concentrando en uno de ellos los compuestos con actividad antimicrobiana.  &nbsp

Supercritical Carbon Dioxide Antisolvent Fractionation for the Sustainable Concentration of Lavandula luisieri (Rozeira) Riv.- Mart Antimicrobial and Antioxidant Compounds and Comparison with Its Conventional Extracts

Lavandula stoechas subsp. luisieri is a Spanish subspecies from the Lamiaceae family. Its essential oil has been traditionally used for several medical applications though little is known about other extracts. Similar to many other studies aiming to obtain traditional plant extracts to be used in di_erent applications, this work evaluated the antioxidant and antimicrobial activities of Lavandula luisieri extracts and the correlation with their composition. Traditional hydrodistillation and ethanolic maceration were used to obtain the essential oil and the maceration extract, respectively. A green and sustainable methodology was applied to the maceration extract that was under a Supercritical Antisolvent Fractionation process to obtain a fine solid enriched in rosmarinic acid and the terpenes oleanolic and ursolic acids. Antimicrobial activities of all extracts and pure identified compounds (rosmarinic and ursolic acids) were evaluated against five bacterial strains; Listeria monocytogenes, Enterococcus faecium, Staphylococcus aureus, Salmonella Typhimurium and Escherichia coli and were compared with the pure compounds identified, rosmarinic and ursolic acids. All strains were sensitive against L. luisieri essential oil. The solid product obtained from the supercritical process was concentrated in the identified actives compared to the maceration extract, which resulted in higher antimicrobial and DPPH scavenging activities. The supercritical sustainable process provided L. luisieri compounds, with retention of their antimicrobial and antioxidant activities, in a poder exemptof organic solvents with potential application in the clinical, food or cosmetic fields