AccPbFRET: An ImageJ plugin for semi-automatic, fully corrected analysis of acceptor photobleaching FRET images

<p>Abstract</p> <p>Background</p> <p>The acceptor photobleaching fluorescence resonance energy transfer (FRET) method is widely used for monitoring molecular interactions in cells. This method of FRET, while among those with the simplest mathematics, is robust, self-controlled and independent of fluorophore amounts and ratios.</p> <p>Results</p> <p>AccPbFRET is a user-friendly, efficient ImageJ plugin which allows fully corrected, pixel-wise calculation and detailed, ROI (region of interest)-based analysis of FRET efficiencies in microscopic images. Furthermore, automatic registration and semi-automatic analysis of large image sets is provided, which are not available in any existing FRET evaluation software.</p> <p>Conclusion</p> <p>Despite of the widespread applicability of the acceptor photobleaching FRET technique, this is the first paper where all possible sources of major errors of the measurement and analysis are considered, and AccPbFRET is the only program which provides the complete suite of corrections – for registering image pairs, for unwanted photobleaching of the donor, for cross-talk of the acceptor and/or its photoproduct to the donor channel and for partial photobleaching of the acceptor. The program efficiently speeds up the analysis of large image sets even for novice users and is freely available.</p

Sorbanállási rendszerek a számítástudományban, távközlésben és megbízhatóság elméletben = Queueing Theory in Computer Sciences, Telecommunications, and Reliability Theory

Az elért eredmények rövid ismertetése: Kutatásainkat lényegében a tervnek megfelelően végeztük. Összességében: 15 nemzetközi folyóirat cikk, számos nemzetközi konferencia előadás, 18 nemzetközi konferencia kiadványban megjelent cikk. Sztrik János szakmai együttműködés keretében végzett kutatásokat Dél-Koreában, Németországban, Grúziában. Lakatos László számos konferencián tartott előadást. Elért eredményeink: I.Bonyolult rendszerek megbízhatósági vizsgálatai 1. A Pollaczek-Hincsin formula vizsgálata ciklikus rendszerekben 2. Véges-forrású, visszatétő igényeket tartalmazó sorbanállási rendszerek alkalmazása önszerveződő rendszereknél a MOSEL szoftver segítségével 3. Strukturált Markov-láncok analízise, különös tekintettel a véges-forrású sorbanállási rendszerekre II. Infokommunikációs hálózatok hatékonyságvizsgálata 1. Szétosztott protokollok modellezése 2. Proxy - Cache szerverek hatékonysági vizsgálatai nem-megbízható kiszolgálók esetén 3. Aszimmetrikus hálózati protokollok működésének modellezése 4. DiffServ modellek összehasonlítása szimuláció és emuláció segítségével Minden jog fenntartva ©, Országos Tudományos Kutatási Alapprogramok | The researcj was carried iut according to the plan. The accepted project was essentially fullfilled. In summary: 15 papers in international journals, several conference presentations, 18 papers in international conference Proceedings. János Sztrik carried researh in Korea, Germany and Georgia. László Lakatos took part several international conference. Obtained results: I. Reliability investigations of complex systems 1. Pollaczek-Khintchine formalas in cyclic systems 2. Modelling of self-organizing systems by the help of finite-source retrial queueing systems via MOSEL 3. Structured Markov-chains with special respect to finite-source retrial queues II. Performance evaluation of info-communication systems 1. Modelling of distributed protocols 2. Performance of Proxy-Cache server systems with unreliable servers 3. Modelling of asymmetric network protocols 4. Comparing DiffServ models via simulation and emulation

Heterogeneous Finite-Source Retrial Queues with Server Subject to Breakdowns and Repairs

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J. ROSZIK et al.: RETRIAL QUEUES IN THE PERFORMANCE MODELING OF... RETRIAL QUEUES IN THE PERFORMANCE MODELING OF CELLULAR MOBILE NETWORKS USING MOSEL

Abstract: This paper investigates a multiserver infinite-source retrial queueing system for the performance modeling of cellular mobile communication networks. The objective is to demonstrate how performance tool MOSEL (Modeling, Specification and Evaluation Language) can be efficiently used in the modeling of cell based networks. In our analysis the blocked and dropped users are treated separately, that is they redial with different probabilities and different rates, with reducing the state space by maximizing the number of redialing customers with appropriately large values (i. e. when the ignored probability mass can be neglected). The guard channel scheme is included in the model, too. The novelty of our analysis is that not only the active but also both types of redialing customers are allowed to depart to other cells, which was not the case in the previous works. The model description is translated step by step into the description language of MOSEL, and then it is automatically converted into the other tool-specific system descriptions and analyzed by the appropriate tools. As the benefit of the tool the effects of various system parameters on the fresh call blocking probability, on the handoff call dropping probability and on the grade of service are displayed and analyzed graphically