39 research outputs found
Fluorescence microscopy for studying the viability of micro-organisms in natural whey starters
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An alternative approach to the yeast extract-nalidixic acid method for determining the proportion of metabolically active aquatic bacteria
No full textUse of Polymerase Chain Reaction and Restriction Enzyme Analysis to directly detect and identify Salmonella typhimurium
No full textDetection and identification of E. coli producing heat-labile enterotoxin type I by enzymatic amplification of a specific DNA fragment
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Molecular vs culture methods for the detection of bacterial faecal indicators in groundwater for human use
No full textFiuorimetric assessment of Pseudomonas fluorescens viability after freeze-thawing using ethidium bromide
No full textSpecificity of substrate recognition by type II dehydroquinases as revealed by binding of polyanions
Get PDFThe interactions between the polyanionic ligands phosphate and sulphate and the type II dehydroquinases from <i>Streptomyces coelicolor</i> and <i>Mycobacterium tuberculosis</i> have been characterised using a combination of structural and kinetic methods. From both approaches, it is clear that interactions are more complex in the case of the latter enzyme. The data provide new insights into the differences between the two enzymes in terms of substrate recognition and catalytic efficiency and may also explain the relative potencies of rationally designed inhibitors. An improved route to the synthesis of the substrate 3-dehydroquinic acid (dehydroquinate) is described
