Comparison of two enzymatic immunoassays, high resolution mass spectrometry method and radioimmunoassay for the quantification of human plasma histamine

International audienceHistamine (HA) is one of the main immediate mediators involved in allergic reactions. HA plasma concentration is well correlated with the severity of vascular and respiratory signs of anaphylaxis. Consequently, plasma quantification of HA is useful to comfort the diagnosis of anaphylaxis. Currently, radioimmunoassay (RIA) is the gold standard method to quantify HA due to its high sensitivity, but it is time consuming, implicates specific formations and cautions for technicians, and produces hazardous radioactive wastes. The aim of this study was to compare two enzymatic immunoassays (EIA) and one in-house liquid chromatography high-resolution mass spectrometry method (LC-HRMS) with the gold standard method for HA quantification in plasma samples of patients suspected of anaphylaxis reactions. Ninety-two plasma samples were tested with the 4 methods (RIA, 2 EIA and LC-HRMS) for HA quantification. Fifty-eight samples displayed HA concentrations above the positive cut-off of 10 nM evaluated by RIA, including 18 highly positive samples (>100 nM). Our results showed that Immunotech® EIA and LC-HRMS concentrations were highly correlated with RIA values, in particular for samples with a HA concentration around the positive cut-off. In our hands, plasma concentrations obtained with the Demeditec Diagnostics® EIA correlated less with results obtained by RIA, and an underestimation of plasma HA levels led to a lack of sensitivity. In conclusion, this study demonstrates that Immunotech® EIA and LC-HRMS method could be used instead of RIA to assess plasma HA in human diagnostic use

Le Kava à Wallis et Futuna. Survivance d'un breuvage océanien traditionnel

, . Le Kava à Wallis et Futuna. Survivance d'un breuvage océanien traditionnel. Talence : Centre de Recherches sur les Espaces Tropicaux (Université Michel de Montaigne, Bordeaux 3), 1986. 160 p. (Îles et Archipels, 6

Ko te Evaselio a sagato Maleko. Gospel of Mark in Wallis

Rossille Richard. Ko te Evaselio a sagato Maleko. Gospel of Mark in Wallis. In: Journal de la Société des océanistes, n°37, tome 28, 1972. pp. 362-363

Ko te Evaselio a sagato Maleko. Gospel of Mark in Wallis

Rossille Richard. Ko te Evaselio a sagato Maleko. Gospel of Mark in Wallis. In: Journal de la Société des océanistes, n°37, tome 28, 1972. pp. 362-363

Godard Philippe, Wallis et Futuna

Rossille Richard, Gaillot M. Godard Philippe, Wallis et Futuna. In: Journal de la Société des océanistes, n°54-55, tome 33, 1977. p. 108

Godard Philippe, Wallis et Futuna

Rossille Richard, Gaillot M. Godard Philippe, Wallis et Futuna. In: Journal de la Société des océanistes, n°54-55, tome 33, 1977. p. 108

Integrating clinical, gene expression, protein expression and preanalytical data for in silico cancer research.

International audienceWe present the phase I development of an integrative platform for the analysis of clinical, gene expression, protein expression and pre-analytical data. The platform is aimed at providing transparent access and analysis tools to researchers investigating new biomarkers and prognosis factors in the particular field of lymphoma diseases. In this article, we report on the data integration phase. The platform's principal advantage is its completeness as it integrates in a single environment clinical, genomic and proteomic data, allowing for their combined analysis. The architecture consists in a data warehouse including data on patients, clinical trials and array platforms and a DeMilitarized Zone for data exchange. A secure web-based platform allows any collaborative team to request the data warehouse and access basic statistics on integrated data. The presented system is currently in use