Uncertainty quantification of leakages in a multistage simulation and comparison with experiments

The present paper presents a numerical study of the impact of tip gap uncertainties in a multistage turbine. It is well known that the rotor gap can change the gas turbine efficiency but the impact of the random variation of the clearance height has not been investigated before. In this paper the radial seals clearance of a datum shroud geometry, representative of steam turbine industrial practice, was systematically varied and numerically tested. By using a Non-Intrusive Uncertainty Quantification simulation based on a Sparse Arbitrary Moment Based Approach, it is possible to predict the radial distribution of uncertainty in stagnation pressure and yaw angle at the exit of the turbine blades. This work shows that the impact of gap uncertainties propagates radially from the tip towards the hub of the turbine and the complete span is affected by a variation of the rotor tip gap. This amplification of the uncertainty is mainly due to the low aspect ratio of the turbine and a similar behavior is expected in high pressure turbines

Properties, Engineering and Applications of PolymericNanofibers: Current Research and Future Advances

The subject of nanomedicine has seen a surge in research activity over the past decade, with nanofibers being a particularly active field. Nanofibers are solid, dry fibers with nanometer diameters, made of various polymers, whereas electrospinning is a versatile, simple, elegant, reproducible, continuous and scalable technology for their preparation. Nanofibers are a unique class of materials in the biomedical field, since they provide a biomimetic environment on the nanometer scale, a three-dimensional architecture with the desired surface properties on the micrometer scale, combined with mechanical strength and physiological acceptability on the macro scale. In particular, their ability to imitate the fibrillar elements of a natural extracellular matrix in a very realistic way is crucial. In this paper we introduce the fundamental aspects of the electrospinning process and the properties of nanofibers, as well as highlighting the enormous potential of nanofibers as drug-delivery systems and tissue scaffolds

Recurrent acquisition of cytosine methyltransferases into eukaryotic retrotransposons

Transposable elements are in a constant arms race with the silencing mechanisms of their host genomes. One silencing mechanism commonly used by many eukaryotes is dependent on cytosine methylation, a covalent modification of DNA deposited by C5 cytosine methyltransferases (DNMTs). Here, we report how two distantly related eukaryotic lineages, dinoflagellates and charophytes, have independently incorporated DNMTs into the coding regions of distinct retrotransposon classes. Concomitantly, we show that dinoflagellates of the genus Symbiodinium have evolved cytosine methylation patterns unlike any other eukaryote, with most of the genome methylated at CG dinucleotides. Finally, we demonstrate the ability of retrotransposon DNMTs to methylate CGs de novo, suggesting that retrotransposons could self-methylate retrotranscribed DNA. Together, this is an example of how retrotransposons incorporate host-derived genes involved in DNA methylation. In some cases, this event could have implications for the composition and regulation of the host epigenomic environment

Modified divergence theorem for analysis and optimization of wall reflecting cylindrical UV reactor

In this paper, the modified divergence theorem (MDT), known in earlier literature as the Gauss-Ostrogradsky theorem, was formulated and proposed as a general approach to electromagnetic (EM) radiation, especially ultraviolet (UV) radiation reactor modeling. A formulated mathematical model, based on MDT, for a multilamp UV reactor was applied to all sources in a reactor in order to obtain intensity profiles at chosen surfaces inside the reactor. Applied modification of MDT means that intensity at a real opaque or transparent surface or through a virtual surface, opened or closed, from different sides of the surface are added and not subtracted as in some other areas of physics. The derived model is applied to an example of the multiple UV sources reactor, where sources are arranged inside a cylindrical reactor at the coaxial virtual cylinder, having the radius smaller than the radius of the reactor. In this work, optimization of a reactor means maximum transfer of EM energy sources into the fluid for given fluid absorbance and fluid flow-dose product. The obtained results, for water quality known in advance, give a unique solution for an optimized model of a multilamp reactor geometry. As everyone can easily verify, MDT is a very good starting point for every reactor modeling and analysis

Direct Transcriptional Consequences of Somatic Mutation in Breast Cancer.

Disordered transcriptomes of cancer encompass direct effects of somatic mutation on transcription, coordinated secondary pathway alterations, and increased transcriptional noise. To catalog the rules governing how somatic mutation exerts direct transcriptional effects, we developed an exhaustive pipeline for analyzing RNA sequencing data, which we integrated with whole genomes from 23 breast cancers. Using X-inactivation analyses, we found that cancer cells are more transcriptionally active than intermixed stromal cells. This is especially true in estrogen receptor (ER)-negative tumors. Overall, 59% of substitutions were expressed. Nonsense mutations showed lower expression levels than expected, with patterns characteristic of nonsense-mediated decay. 14% of 4,234 rearrangements caused transcriptional abnormalities, including exon skips, exon reusage, fusions, and premature polyadenylation. We found productive, stable transcription from sense-to-antisense gene fusions and gene-to-intergenic rearrangements, suggesting that these mutation classes drive more transcriptional disruption than previously suspected. Systematic integration of transcriptome with genome data reveals the rules by which transcriptional machinery interprets somatic mutation

Improving Cry8Ka toxin activity towards the cotton boll weevil (Anthonomus grandis)

<p>Abstract</p> <p>Background</p> <p>The cotton boll weevil (<it>Anthonomus grandis</it>) is a serious insect-pest in the Americas, particularly in Brazil. The use of chemical or biological insect control is not effective against the cotton boll weevil because of its endophytic life style. Therefore, the use of biotechnological tools to produce insect-resistant transgenic plants represents an important strategy to reduce the damage to cotton plants caused by the boll weevil. The present study focuses on the identification of novel molecules that show improved toxicity against the cotton boll weevil. <it>In vitro </it>directed molecular evolution through DNA shuffling and phage display screening was applied to enhance the insecticidal activity of variants of the Cry8Ka1 protein of <it>Bacillus thuringiensis</it>.</p> <p>Results</p> <p>Bioassays carried out with <it>A. grandis </it>larvae revealed that the LC₅₀of the screened mutant Cry8Ka5 toxin was 3.15-fold higher than the wild-type Cry8Ka1 toxin. Homology modelling of Cry8Ka1 and the Cry8Ka5 mutant suggested that both proteins retained the typical three-domain Cry family structure. The mutated residues were located mostly in loops and appeared unlikely to interfere with molecular stability.</p> <p>Conclusions</p> <p>The improved toxicity of the Cry8Ka5 mutant obtained in this study will allow the generation of a transgenic cotton event with improved potential to control <it>A. grandis</it>.</p

Rearrangement bursts generate canonical gene fusions in bone and soft tissue tumors

Sarcomas are cancers of the bone and soft tissue often defined by gene fusions. Ewing sarcoma involves fusions between EWSR1, a gene encoding an RNA binding protein, and E26 transformation-specific (ETS) transcription factors. We explored how and when EWSR1-ETS fusions arise by studying the whole genomes of Ewing sarcomas. In 52 of 124 (42%) of tumors, the fusion gene arises by a sudden burst of complex, loop-like rearrangements, a process called chromoplexy, rather than by simple reciprocal translocations. These loops always contained the disease-defining fusion at the center, but they disrupted multiple additional genes. The loops occurred preferentially in early replicating and transcriptionally active genomic regions. Similar loops forming canonical fusions were found in three other sarcoma types. Chromoplexy-generated fusions appear to be associated with an aggressive form of Ewing sarcoma. These loops arise early, giving rise to both primary and relapse Ewing sarcoma tumors, which can continue to evolve in parallel