55 research outputs found
Mixed Thymoma in a Young Cynomolgus Monkey (Macaca fascicularis)
A mass with a diameter of 1.5 cm was found in the thymus of a 4-year and
3-month-old male cynomolgus monkey. Microscopically, the mass consisted of two
different patterns of proliferation, dense or fascicular proliferation of
elongated spindle cells in a sporadic storiform pattern and dense proliferation
of thymic cortex-like lymphoid cells in which the multifocal pale nests
resembling the thymic medulla were distributed. In these pale nests, large
dendriform cells sometimes forming Hassall’s corpuscles were present. The
proliferating spindle cells were positive for cytokeratin. The lymphoid cells in
the mass were positive for CD3. We concluded that the mass consisted of the
neoplastic thymic epithelium with thymocytes proliferation containing medullary
differentiation. The mass was diagnosed as a mixed thymoma according to the WHO
classification of thymomas in humans. Mixed thymoma is characterized as a
mixture of two types of proliferative lesions, spindle-shaped epithelial
proliferation and a lymphocyte predominant lesion with or without medullary
differentiation. To the best of our knowledge, this is the first report
concerning thymoma in monkeys
Genotypic characterization of psittacid herpesvirus isolates from Brazil
AbstractThirty-six isolates of psittacid herpesvirus (PsHV), obtained from 12 different species of psittacids in Brazil, were genotypically characterized by restriction fragment length polymorphism (RFLP) analysis and PCR amplification. RFLP analysis with the PstI enzyme revealed four distinct restriction patterns (A1, X, W and Y), of which only A1 (corresponding to PsHV-1) had previously been described. To study PCR amplification patterns, six pairs of primers were used. Using this method, six variants were identified, of which, variants 10, 8, and 9 (in this order) were most prevalent, followed by variants 1, 4, and 5. It was not possible to correlate the PCR and RFLP patterns. Twenty-nine of the 36 isolates were shown to contain a 419bp fragment of the UL16 gene, displaying high similarity to the PsHV-1 sequences available in GenBank. Comparison of the results with the literature data suggests that the 36 Brazilian isolates from this study belong to genotype 1 and serotype 1
POST-HEPATIC JAUNDICE ASSOCIATED WITH PLATINOSOMIASIS IN A CAT
A platinossomíase é uma infecção parasitária causada pelo trematódeo Platynosomum spp., encontrado no fígado de gatos domésticos. O felino é o hospedeiro definitivo e a severidade da doença depende da quantidade de parasitos, tempo de parasitismo e resposta do hospedeiro. O objetivo deste trabalho foi relatar um caso de icterícia pós-hepática em um gato decorrente de platinossomíase. Um gato, macho, jovem, sem raça definida, foi recebido para exame pós morte com histórico de icterícia grave, emagrecimento progressivo e morte repentina. Uma amostra de conteúdo biliar foi submetida ao método de sedimentação por centrifugação para a busca de ovos de helmintos, que revelou numerosos ovos marrom-escuros, ovais, monoperculados e embrionados, morfologicamente compatíveis com ovos de Platynosomum. O exame direto do fígado em estereoscópio e a histopatologia confirmaram a parasitose sob a identificação de formas adultas do parasito que apresentavam características morfológicas compatíveis com Platynosomum illiciens. As lesões histológicas incluíram colangio-hepatite, ectasia com obstrução ductal, fibrose, regeneração micronodular e lipidose. A icterícia pós-hepática foi relacionada à estase biliar resultante do acúmulo de muco e parasitos. Neste caso, os achados macroscópicos (icterícia intensa, fibrose hepática e colestase extra-hepática obstrutiva) e histológicos associados à identificação de parasitos adultos recuperados dos ductos biliares foram fundamentais para o diagnóstico definitivo da doença.Platinosomiasis is a parasitic infection caused by the trematode Platynosomum spp., found in the liver of domestic felines. The feline is the definitive host of Platynosomum, and the severity of the disease depends on the amount of parasites, time of parasitism and host response. The aim of this work was to report a case of post-hepatic jaundice resulting from feline platinosomiasis. A male, young, mixed breed feline was presented with a history of severe jaundice, progressive weight loss and sudden death. A sample of the bile content was collected and analyzed by sedimentation through centrifugation to search for helminth eggs, resulting in the observation of innumerous dark brown, oval, monoperculated and embryonated eggs, morphologically compatible with eggs of Platynosomum. The stereoscopy of fresh liver samples and histopathology confirmed the parasitism through the identification of adult specimens, whose morphological features were compatible with Platynosomum illiciens. The post-hepatic jaundice was related to bile stasis resulting from the accumulation of intraductal mucus and parasites. In this particular case, the pathological findings associated with the identification of adults recovered from the biliary content were essential for the definitive diagnosis of the disease
Icterícia pós-hepática associada à platinossomíase em um gato
A platinossomíase é uma infecção parasitária causada pelo trematódeo Platynosomum spp., encontrado no fígado de gatos domésticos. O felino é o hospedeiro definitivo e a severidade da doença depende da quantidade de parasitos, tempo de parasitismo e resposta do hospedeiro. O objetivo deste trabalho foi relatar um caso de icterícia pós-hepática em um gato decorrente de platinossomíase. Um gato, macho, jovem, sem raça definida, foi recebido para exame pós morte com histórico de icterícia grave, emagrecimento progressivo e morte repentina. Uma amostra de conteúdo biliar foi submetida ao método de sedimentação por centrifugação para a busca de ovos de helmintos, que revelou numerosos ovos marrom-escuros, ovais, monoperculados e embrionados, morfologicamente compatíveis com ovos de Platynosomum. O exame direto do fígado em estereoscópio e a histopatologia confirmaram a parasitose sob a identificação de formas adultas do parasito que apresentavam características morfológicas compatíveis com Platynosomum illiciens. As lesões histológicas incluíram colangio-hepatite, ectasia com obstrução ductal, fibrose, regeneração micronodular e lipidose. A icterícia pós-hepática foi relacionada à estase biliar resultante do acúmulo de muco e parasitos. Neste caso, os achados macroscópicos (icterícia intensa, fibrose hepática e colestase extra-hepática obstrutiva) e histológicos associados à identificação de parasitos adultos recuperados dos ductos biliares foram fundamentais para o diagnóstico definitivo da doença
Performance, nutrient digestibility, and muscular evaluation of female broiler chickens fed different dietary protein levels and slaughtered at 38 or 46 days
ABSTRACT This study evaluated the effects of two protein levels and types of formulation on performance, nutrient metabolization, and myopathies of 390 female broilers from 21 to 46 days old distributed in a completely randomized design, with five treatments and six replicates of 13 broilers each. Treatments were two levels of protein (19.0 and 21.0%) in diets using only commercially available ingredients and three experimental formulations designed to keep similar the main nutrients that could influence nutritional performance (starch, fiber, ether extract), using unusual ingredients, with 19.0% (19E) or 21.0% (21E) crude protein and the third with 19.0% crude protein with amino acid levels similar to treatment 21P (19E+Aa). We studied broiler performance, nutrient digestibility, macroscopic muscular evaluation, and histological muscular evaluation. The design for the performance and myopathy assessment was a 5 × 2 factorial scheme, five diets × two ages (38 or 46 days). The worst feed conversion ratio was observed for the 19P treatment. Neither diet nor age had an effect on myopathies, however, older slaughter age increased the intensity of microscopic lesions. The diet 19P should be avoided and increased inclusion of lipids in diets improves nutrient utilization and, consequently, performance
Histomorphological and immunohistochemical characterization of 172 cutaneous round cell tumours in dogs
Outbreak of infectious laryngotracheitis in large multi-age egg layer chicken flocks in Minas Gerais, Brazil
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