Expression of Msx1 and Dlx1 during Dumbo rat head development: Correlation with morphological features

The Dumbo rat possesses some characteristics that evoke several human syndromes, such as Treacher-Collins: shortness of the maxillary, zygomatic and mandibular bones, and low position of the ears. Knowing that many homeobox genes are candidates in craniofacial development, we investigated the involvement of the Msx1 and Dlx1 genes in the Dumbo phenotype with the aim of understanding their possible role in abnormal craniofacial morphogenesis and examining the possibility of using Dumbo rat as an experimental model for understanding abnormal craniofacial development. We studied the expression of these genes during craniofacial morphogenesis by RT-PCR method. We used Dumbo embryos at E12 and E14 and included the Wistar strain as a control. Semi-quantitative PCR analysis demonstrated that Msx1 and Dlx1 are expressed differently between Dumbo and Wistar rats, indicating that their low expression may underly the Dumbo phenotype

Use of embedded strain gages for the in-vitro study of proximal tibial cancellous bone deformation during knee flexion-extension movement: development, reproducibility and preliminary results of feasibility after frontal low femoral osteotomy

<p>Abstract</p> <p>Background</p> <p>This paper reports the development of an in-vitro technique allowing quantification of relative (not absolute) deformations measured at the level of the cancellous bone of the tibial proximal epiphysis (CB_TPE) during knee flexion-extension. This method has been developed to allow a future study of the effects of low femoral osteotomies consequence on the CB_TPE.</p> <p>Methods</p> <p>Six strain gages were encapsulated in an epoxy resin solution to form, after resin polymerisation, six measurement elements (ME). The latter were inserted into the CB_TPEof six unembalmed specimens, just below the tibial plateau. Knee motion data were collected by three-dimensional (3D) electrogoniometry during several cycles of knee flexion-extension. Intra- and inter-observer reproducibility was estimated on one specimen for all MEs. Intra-specimen repeatability was calculated to determine specimen's variability and the error of measurement. A varum and valgum chirurgical procedure was realised on another specimen to observed CB_TPEdeformation after these kind of procedure.</p> <p>Results</p> <p>Average intra-observer variation of the deformation ranged from 8% to 9% (mean coefficient of variation, MCV) respectively for extension and flexion movement. The coefficient of multiple correlations (CMC) ranged from 0.93 to 0.96 for flexion and extension. No phase shift of maximum strain peaks was observed. Inter-observer MCV averaged 23% and 28% for flexion and extension. The CMC were 0.82 and 0.87 respectively for extension and flexion. For the intra-specimen repeatability, the average of mean RMS difference and the mean ICC were calculated only for flexion movement. The mean RMS variability ranged from 7 to 10% and the mean ICC was 0.98 (0.95 - 0.99). A Pearson's correlation coefficient was calculated showing that RMS was independent of signal intensity. For the chirurgical procedure, valgum and varum deviation seems be in agree with the frontal misalignment theory.</p> <p>Conclusions</p> <p>Results show that the methodology is reproducible within a range of 10%. This method has been developed to allow analysis the indirect reflect of deformation variations in CB_TPEbefore and after distal femoral osteotomies. The first results of the valgum and varum deformation show that our methodology allows this kind of measurement and are encourageant for latter studies. It will therefore allow quantification and enhance the understanding of the effects of this kind of surgery on the CB_TPEloading.</p

Expression of Msx1 and Dlx1 during Dumbo rat head development: Correlation with morphological features

The Dumbo rat possesses some characteristics that evoke several human syndromes, such as Treacher-Collins: shortness of the maxillary, zygomatic and mandibular bones, and low position of the ears. Knowing that many homeobox genes are candidates in craniofacial development, we investigated the involvement of the Msx1 and Dlx1 genes in the Dumbo phenotype with the aim of understanding their possible role in abnormal craniofacial morphogenesis and examining the possibility of using Dumbo rat as an experimental model for understanding abnormal craniofacial development. We studied the expression of these genes during craniofacial morphogenesis by RT-PCR method. We used Dumbo embryos at E12 and E14 and included the Wistar strain as a control. Semi-quantitative PCR analysis demonstrated that Msx1 and Dlx1 are expressed differently between Dumbo and Wistar rats, indicating that their low expression may underly the Dumbo phenotype

The effects of the Dh gene on limb morphogenesis in the mouse

SCOPUS: re.jinfo:eu-repo/semantics/publishe

Etude de la genèse des malformations des membres provoquées chez la souris par l'administration de deux agents teratogènes: l'hadacidine et la cytosine arabinoside

Doctorat en sciences médicalesinfo:eu-repo/semantics/nonPublishe

Principes et concepts de la tératogénèse. I. L'agression tératogène.

Comparative StudyEnglish AbstractJournal Articleinfo:eu-repo/semantics/publishe

Correlations between necrotic patterns and limb skeletal defects induced by antimitotic drugs in the mouse.

Journal Articleinfo:eu-repo/semantics/publishe

Démonstrations d'anatomie humaine :travaux pratiques de dissection

2e cand. Educ. phys. et Kinés. - ANAT 060info:eu-repo/semantics/published

Démonstrations d'anatomie humaine :travaux pratiques de dissection

Première édition 1982-19832e candid. éducation Physique et Kinésithérapieinfo:eu-repo/semantics/published

ANALYSE DES LESIONS SQUELETTIQUES DES MEMBRES POSTERIEURS CHEZ LES SOURIS Dh/+

The skeletal anomalies of the hind limbs were studied on 100 youth mice +heterozygotes concerning the Dh gene (Dominant Hemimelia). The gene is dominant, not sexlinked and provokes apart from the skeletal malformations, a constant agenesia of the spleen and a microstomach. The fore limb is not affected in the heterozygotes animals. The limbs were coloured by the alizarin sulfonic acid, according to Dawson's method, and examined in pure glycerin. A study of the articulation of the instep on microscopic slides after Masson's trichromic coloration was made on animals affected by partial or total hemimelia. The greater part of the skeletal lesions may be classified into 2 groups: anomalies by excess (hyperphalangism or preaxial polydactylism) and anomalies by defect (total or partial hemimelia of the zeugopode). These 2 malformations are not associated in the same limb although they can be found associated in both limbs of the same embryo. There is an exception to this rule: the case of syndactylism I-II that implies some excess lesions, e.g. a supernumerary phalanx, and preaxial hemimelia in the same limb. The expressivity of the gene is non existant at the hind limb level in 9% of the cases.SCOPUS: NotDefined.jinfo:eu-repo/semantics/publishe