PERBEDAAN JUMLAH ANGKA KUMAN UDARA SEBELUM DAN SESUDAH PENGGUNAAN DUA ULTRAVIOLET TUBE DI RUANG LABORATORIUM BAKTERIOLOGI JURUSAN ANALIS KESEHATAN

INTISARI Latar Belakang : Kuman kontaminan yang berasal dari udara dapat menggagu ketepatan pemeriksaan sampel bakteriologi klinis. Ultraviolet tube digunakan untuk menurunkan angka kuman udara dalam ruangan dapat digunakan untuk mencegah kontaminasi pemeriksaan bakteriologi di laboratorium klinik, khususnya laboratorium mikrobiologi. Penggunaan ultraviolet tube memiliki kelebihan ketika proses penurunan angka kuman ruangan tetap dapat digunakan untuk aktivitas. Tujuan Penelitian : Penelitian ini bertujuan untuk mengetahui perbedaan jumlah angka kuman udara sebelum dan sesudah penggunaan dua ultraviolet tube di ruang laboratorium jurusan analis kesehatan. Metode Penelitian : Jenis Penelitian ini adalah pre-experimental designs dengan rancangan one group pretest-postest design. Objek penelitian ini adalah udara di ruang laboratorium bakteriologi dan subjek penelitian ini adalah kuman yang tumbuh di media PCA, Penelitian ini dilakukan 16 kali pengulangan sebelum maupun sesudah penggunaan dua ultraviolet tube. Data yang diperoleh dianalisis secara statistik menggunakan uji parametrik Paired Sampel T-Test dengan derajat kepercayaan 95% menggunakan SPSS 16.0 for windows. Hasil Penelitian : Rata-rata jumlah angka kuman udara sebelum penggunaan dua ultraviolet tube sebesar 292 CFU/m3 dan rerata jumlah angka kuman sesudah penggunaan dua ultraviolet tube sebesar 31 CFU/m3. Persentase rata-rata penurunan angka kuman sesudah penggunaan dua ultraviolet tube sebesar 84%. Uji statistic Parametrik Paired Sampel T-Test diperoleh hasil Asymp.Sig (2-tailed) sebesar 0,000 Kesimpulan : Ada perbedaan signifikan jumlah angka kuman udara sebelum dan sesudah penggunaan dua ultraviolet tube di ruang laboratorium Jurusan Analis Kesehatan. Kata kunci : Angka Kuman Udara, Ultraviolet Tube, Laboratoriu

PERBEDAAN JUMLAH ANGKA KUMAN UDARA SEBELUM DAN SESUADAH PENGGUNAAN DUA ULTRAVIOLET TUBE DI RUANG LABORATORIUM JURUSAN ANALIS KESEHATAN

Background : Air originated contaminating bacteria can interfere the precision of clinical bacteriology sample check. Ultraviolet tube can be used to avoid bacteriology laboratory check from contamination especially microbiology laboratory. The we of ultraviolet tube has an advantage in which it still can be used while in the process of reducing the number of bacteria in the air. Objectives of study : This study is a pre-experimental study with one group pretestpostest design. The object of the study was the air of bacteriology laboratory and subject of the study were grown bacteria in PCA media. This study was executed 16 times repetition before and after the use of ultraviolet tube. The obtained data were analyzed statistically by using parametric test, that is paired sample t – test with confidance interval is 95%. The analyzing data was used SPSS 16.0 for windows. The result of study : The average of the number of bacteria in the air before using ultraviolet tube is 292 CFU/m3 and after using ultraviolet tube is 31 CFU/m3. The everage percentage of the number of bacteria reduction after using two ultraviolet tube is 84 %. From paired sample t-test, it was obtained Asymp sig (2-tailed) as many as 0,000. Conclusion : There is significant difference between the number of bacteria in the air before and after using ultraviolet tube in laboratory in Analis Kesehatan Major Keywords : The number of bacteria in the air, ultraviolet tube, laborator

ANGKA KUMAN UDARA SEBELUM DAN SETELAH DISINARI LAMPU UV DENGAN DAYA 60 WATT DI LABORATORIUM BAKTERIOLOGI JURUSAN ANALIS KESEHATAN POLTEKKES KEMENKES YOGYAKARTA

Latar Belakang : Jumlah angka kuman paling tinggi terdapat di laboratorium Bakteriologi, karena merupakan ruangan yang digunakan untuk praktikum secara kontinu dengan melibatkan sampel dan media yang mengandung bakteri dalam jumlah besar Pengendalian kuman udara yang menyebabkan kontaminasi dan mengganggu pemeriksaan sampel mikrobiologi dapat dilakukan dengan berbagai cara salah satunya dengan menggunakan sinar UV. UV-C dengan panjang gelombang antara 200 nm dan 280 nm adalah wilayah germicidal yang efektif untuk membunuh bakteri Tujuan Penelitian : Penelitian ini bertujuan untuk Mengetahui adanya perbedaan angka kuman udara diruang laboratorium bakteriologi jurusan analis kesehatan sebelum dan setelah dipaparkan lampu UV dengan daya 60 watt. Metode penelitian : Jenis penelitian ini adalah Quasi eksperiment, dengan desain penelitian One Group Pre-post test Design. Objek penelitian ini adalah udara di ruang laboratorium bakteriologi jurusan analis kesehatan. Penelitian ini dilakukan dengan 16 kali pengulangan sebelum maupun setelah disinari UV. Data yang diperoleh dianalisis secara statistik menggunakan SPSS 16.0 for windows dengan uji parametrik Paired sample T-Test dan dengan derajat kepercayaan 95%. Hasil Penelitian : Rerata angka kuman udara sebelum penyinaran 6227 CFU/M3 dan rerata setelah penyinaran adalah 1000 CFU/M3. Persentase rerata penurunan angka kuman setelah penyinaran lampu UV dengan daya 60 watt sebesar 75%. Uji parametrik Paired sample T-Test diperoleh hasil Asymp.Sig (2-tailed) yaitu 0.004. Kesimpulan : Ada perbedaan angka kuman udara diruang laboratorium bakteriologi jurusan analis kesehatan sebelum dan setelah dipaparkan lampu UV dengan daya 60 watt Kata Kunci : sinar UV, Angka kuman udara, Laboratorium bakteriologi

EFEKTIVITAS SEDUHAN HERBAL SERAI (Cymbopogon citratus) TERHADAP KADAR ASAM URAT DARAH TIKUS PUTIH (Rattus norvegicus) JANTAN GALUR WISTAR YANG DIINDUKSI STREPTOZOTOCIN (STZ)

Latar Belakang : Diabetes Mellitus adalah penyakit kronis yang terjadi baik ketika pankreas tidak menghasilkan cukup insulin atau ketika tubuh tidak dapat secara efektif menggunakan insulin yang dihasilkannya. Peningkatan kadar asam urat berhubungan dengan hiperinsulinemia pada pasien Diabetes Mellitus Tipe 2 yang dapat memicu timbulnya komplikasi hiperurisemia dan oleh karena itu insulin juga berperan dalam meningkatkan reabsorpsi asam urat. Serai mengandung flavonoid, alkaloid, saponin, dan tannin yang efektif sebagai inhibitor xanthin oksidase. Penghambatan xanthin oksidase dapat menghalangi biosintesis asam urat yang dapat menjadi hiperurisemia. Tujuan Penelitian : mengetahui efektivitas seduhan herbal serai terhadap penurunan kadar asam urat darah tikus putih galur Wistar yang diinduksi streptozotocin Metode Penelitian : penelitian ini menggunakan tikus sebanyak 30 ekor dibagi menjadi 6 kelompok. Kelompok 1 sebagai kontrol negatif, kelompok 2 sebagai kontrol positif, kelompok 3 diberikan glibenklamid, kelompok 4 diberikan seduhan herbal serai dosis 0,09g / 200g BB, dan kelompok 5 diberikan seduhan herbal serai 0,18g / 200g BB selama 28 hari kemudian dilakukan pemeriksaan kadar asam urat. Hasil penelitian : Hasil penelitian menunjukkan rerata kadar asam urat pada kelompok 1 yaitu 1,74 mg/dl, kelompok 2 yaitu 8,30 mg/dl, kelompok 3 yaitu 2,13 mg/dl, kelompok 4 yaitu 3,89 mg/dl, dan kelompok 5 yaitu 2,69 mg/dl. Penurunan kadar asam urat darah dipengaruhi oleh dosis herbal (p<0,05). Kesimpulan : Seduhan herbal serai dapat menurunkan kadar asam urat pada tikus diabetik dan efektivitas penurunan kadar asam urat pada serai tidak lebih baik dibandingkan dengan glibenklamid Kata Kunci : diabetes mellitus, asam urat, sera

Kajian Aktivitas Antikanker Ekstrak Daun Gude (cajanus cajan) Terhadap Sel Kanker Kolon Secara in Vitro

Colorectal cancer is one of the five type of highest insiden in Indonesia. One of development cancer therapeutics directed to combination of chemotherapeutic agent and chemopreventive compounds. One approach to finding a chemopreventive compounds is through the exploration of natural ingredient especialy herbs. Flavonoids is group of compounds from plants that have been widely studied as anti-cancer activity. Leave of pigeonpea (Cajanus cajan) is, kind of Legumes plants rich in flavonoids among others cajanol, quercetin, luteolin, apigenin formonentin, vitexin, orientin, biochanin A, pinostrobin, dan isorhamnetin. This study aims to determine the content of chemical compounds in the methanol extract of leave pigeon pea that has anticancer activity using WiDr colorectal cancer cells with MTT (3-(4, 5-dimethylthiazolyl-2)-2, 5-diphenyltetrazolium bromide) method, followed by flowcytometry test. The result of the cytotoxicity test of methanol extract of pigeonpea leaves have IC50 of 307 ug/ml and with flowcytometry method showed necrosis WiDr cell.Colorectal cancer is one of the five type of highest insiden in Indonesia. One of development cancer therapeutics directed to combination of chemotherapeutic agent and chemopreventive compounds. One approach to finding a chemopreventive compounds is through the exploration of natural ingredient especialy herbs. Flavonoids is group of compounds from plants that have been widely studied as anti-cancer activity. Leave of pigeonpea (Cajanus cajan) is, kind of Legumes plants rich in flavonoids among others cajanol, quercetin, luteolin, apigenin formonentin, vitexin, orientin, biochanin A, pinostrobin, dan isorhamnetin. This study aims to determine the content of chemical compounds in the methanol extract of leave pigeon pea that has anticancer activity using WiDr colorectal cancer cells with MTT (3-(4, 5-dimethylthiazolyl-2)-2, 5-diphenyltetrazolium bromide) method, followed by flowcytometry test. The result of the cytotoxicity test of methanol extract of pigeonpea leaves have IC50 of 307 ug/ml and with flowcytometry method showed necrosis WiDr cell

Kajian Aktivitas Antioksidan Kacang Gude (Cajanus cajan) dan Pengaruhnya Terhadap Aktivitas Enzim Hati Tikus Yang diinduksi Karbon Tetraklorida

Legumes bean is a plant that is rich in compounds called polyphenols, especially flavonoids. Pigeon pea is a legume species that has purple-black skin, contains various compounds, among others, polyphenols, anthocyanins, and flavonoids. Polyphenol, anthocyanins, and flavonoids compounds have an antioxidant&nbsp;activity of endogenous antioxidants which can help to fend off free radicals that occur in the body. The antioxidant activity of pigeon pea (Cajanus cajan) in vitro has measured using DPPH method and obtained results of IC50 value of 70.32 mg/ml. Pigeon pea beans contain anthocyanin compounds of 208.307 mg / 100 g. Measurement of antioxidant activity pigeon pea in vivo was performed by animal model white rats Wistar male as many as 25 animals that were divided into 5 groups. Each experimental group consisted of 5 mice, the group N as a normal control group, the group S as a pain control, the group G1, G2, and G4 were given bean powder pigeon pea respectively 100, 200 and 400 mg / bwt with sonde, for 14 days , S group, G1, G2 and G4 induced by carbon tetrachloride with intraperitoneal administration. After 24 hours, the blood of all rats was taken through vena orbitalis and then the activity of AST, ALT and GGT enzymes and levels of malondialdehyde (MDA) were determined. The result of this study showed inhibition of the increase in the activity of AST, ALT and GGT enzymes and MDA levels in rats fed with powdered pigeon pea. From these results, it can be concluded that pigeon pea beans have antioxidant activity which is capable of preventing damage to liver cells and lipid peroxidation. A result of this study showed inhibition of the increase in the activity of enzymes AST, ALT and GGT and MDA levels in rats fed powdered pigeon pea. From these results, it can be concluded that pigeonpea beans have antioxidant activity which can prevent a damage in Hepar cells and lipid peroxidase

Pengaruh Lama Perendaman Koro Bengu (Mucuna pruriens) Dalam Air Kapur (Ca(OH)2) Terhadap Kadar Asam Siana (HCN)

Background: Indonesian people are still focused on commodity soybeans as the main source of protein, while the use of other commodities such as koro benguk still very minimal. The lack of utilization of this koro benguk because it contains a natural form of glokusida cyanogenic compounds that can undergo enzymatic hydrolysis into hydrogen cyanide (HCN), which is toxic. Because cyanide acid very easily soluble in water, then use the soaking lime-water (Ca(OH)2) is alkaline which was quite effective in neutralizing HCN in koro benguk.Objectives: Determine the effect of soaking time in lime-water koro benguk on levels of cyanide and lime to know if the water is more effective than plain water in neutralizing HCN of koro benguk.Method: Research with post test design with control group. Object of this study was white koro benguk varieties obtained 4-6 months old in Nogosari, Purwosari, District Girimulyo, Kulon Progo, DIY is done soaking in water as a control group and lime-water 100 mg / L as the treatment group during 12, 24 and 36 hours later be distilled. Distillate is reacted with 1% picric acid and then measured the levels of HCN by spectrophotometry.Result: HCN levels koro benguk on soaking in water for 12 hours is 20,736 mg / kg, for 24 hours was 19,348 mg / kg and for 36 hours was 16,786 mg / kg. While the levels of HCN in lime-water soaking of 100 mg / L for 12 hours was 19,020 mg / kg, for 24 hours is 13,635 mg / kg and for 36 hours was 9,307 mg / kg. One way ANOVA test results in the treatment group obtained significant value 0.000 (P &lt;0.05).Conclusion: There was a significant effect of soaking time koro benguk in lime-water on levels of cyanide. Soaking in lime-water to neutralize the cyanide acid to be more effective than soaking in water

Gambaran Hasil Pemeriksaan Aktivitas Enzim Alanine Aminotransferase (ALT) pada Serum Setelah Disimpan Selama 14 Hari: Overview of Alanine Aminotransferase (ALT) Enzyme Activity Assay Result in Serum After Storage for 14 Days

Storage of serum at 4°C over three days causes a 10% decrease in the enzyme Alanine Aminotransferase (ALT) activity. However, several laboratories store remaining serum samples for one week or even one month after examination, which is not under Good Laboratory Practice (GLP). The serum storage is used to anticipate if there is an examination error. This study aims to describe the results of examining ALT enzyme activity in serum that was immediately checked and stored for 14 days. This type of research is descriptive quantitative using the Pretest-Posttest Design. The sample in this study was human blood serum, with a total sample of 10 patients. Data were analyzed descriptively for all data obtained and presented in tabular form. The average yield of ALT enzyme activity examination before storage and storage on day seven and day 14 was 50.7 U/L, 46.1 U/L, and 40.5 U/L. This study concluded that there was a decrease in the results of examining the activity of the ALT enzyme by 9% on the 7th day and 20% on the 14th day. Therefore, it is suggested that laboratory staff check the sample as soon as possible. If there is a need to repeat the analysis, verify the results or add laboratory tests, samples should be frozen to ensure stability

PENGARUH LAMA PERENDAMAN KORO BENGU (Mucuna pruriens) DALAM AIR KAPUR (Ca(OH)2) TERHADAP KADAR ASAM SIANIDA (HCN)

Latar belakang: Masyarakat Indonesia masih menitikberatkan pada komoditas kacang kedelai sebagai sumber utama protein, sedangkan pemanfaatan komoditas lain seperti koro benguk masih sangat minim. Minimnya pemanfaatan koro benguk ini karena di dalamnya terkandung senyawa alami berupa glokusida sianogenik yang dapat mengalami hidrolisis enzimatis menjadi asam sianida (HCN) yang bersifat racun. Karena asam sianida bersifat asam yang sangat mudah larut dalam air, maka dilakukan perendaman menggunakan air kapur (Ca(OH)2) bersifat basa yang dirasa cukup efektif menetralkan HCN dalam koro benguk.   Tujuan Penelitian: Mengetahui pengaruh lama perendaman koro benguk dalam air kapur terhadap kadar asam sianida dan mengetahui apakah air kapur lebih efektif dibandingkan air biasa dalam menetralkan HCN koro benguk.   Metode Penelitian: Penelitian dengan desain post test with control group. Obyek penelitian ini adalah koro benguk varietas benguk putih berumur 4-6 bulan yang diperoleh di Dusun Nogosari, Desa Purwosari, Kecamatan Girimulyo, Kabupaten Kulon Progo, DIY yang dilakukan perendaman dalam air sebagai kelompok kontrol dan air kapur 100 mg/L sebagai kelompok perlakuan selama 12, 24 dan 36 jam kemudian dilakukan destilasi. Destilat direaksikan dengan asam pikrat 1% kemudia diukur kadar HCN secara spektrofotometri.   Hasil: Kadar HCN  koro benguk pada perendaman dalam air selama 12 jam adalah 20,736 mg/kg, selama 24 jam adalah 19,348 mg/kg dan selama 36 jam adalah 16,786 mg/kg. Sedangkan kadar HCN pada perendaman air kapur 100 mg/L selama 12 jam adalah 19,020 mg/kg, selama 24 jam adalah 1,635 mg/kg dan selama 36 jam adalah 9,307 mg/kg. Hasil Uji ANOVA satu jalan pada kelompok perlakuan didapatkan nilai signifikansi 0.000 (p< 0.05).   Kesimpulan: Ada pengaruh bermakna lama perendaman koro benguk dalam air kapur terhadap kadar asam sianida. Perendaman dalam air kapur terbukti lebih efektif menetralkan asam sianida koro benguk dibandingkan perendaman dalam air